RESUMO
The present study aimed to investigate the antibacterial activity of striatisporolide A (SA) against Escherichia coli (E. coli) and the underlying mechanism. Antibacterial activity was evaluated according to the inhibitory rate and zone of inhibition. The antibacterial mechanism was investigated by analyzing alkaline phosphatase (AKP) activity and ATP leakage, protein expression, cell morphology and intracellular alterations in E. coli. The results demonstrated that SA exerted bacteriostatic effects on E. coli in vitro. AKP activity and ATP leakage analysis revealed that SA damaged the cell wall and cell membrane of E. coli. SDSPAGE analysis indicated that SA notably altered the level of 10 and 35 kDa proteins. Scanning electron microscopy and transmission electron microscopy analyses revealed marked alterations in the morphology and ultrastructure of E. coli following treatment with SA. The mechanism underlying the antimicrobial effects of SA against E. coli may be attributed to its actions of disrupting the cell membrane and cell wall and regulation of protein level. The findings of the present study provide novel insight into the antimicrobial activity of SA as a potential natural antibacterial agent.
Assuntos
4-Butirolactona/análogos & derivados , Antibacterianos/química , Escherichia coli/efeitos dos fármacos , Traqueófitas/química , 4-Butirolactona/química , 4-Butirolactona/metabolismo , 4-Butirolactona/farmacologia , Trifosfato de Adenosina/química , Fosfatase Alcalina/genética , Antibacterianos/metabolismo , Antibacterianos/farmacologia , Membrana Celular/efeitos dos fármacos , Membrana Celular/ultraestrutura , Escherichia coli/ultraestrutura , Microscopia Eletrônica de Varredura , Microscopia Eletrônica de Transmissão , Extratos Vegetais/química , Extratos Vegetais/farmacologiaRESUMO
OBJECTIVE: To study the relation between relative density and kinematical viscosity of the concentration for Yuxianling granules. METHOD: The relative density and kinematical viscosity by alkali burette of the concentration were investigated. The connection between kinematical viscosity and spray drying, also and temperature of the concentration was respected. In the meantime, different mathematical models were simulated. RESULT: Kinematical viscosity is susceptible than relative density to the effect for spray drying, and the mathematical model is also set up accordingly. The result can offer the proper inlet temperature and kinematical viscosity for spray drying. CONCLUSIon The method in the experiment is simple, practical and manipulate easily. The study will provide the basis for extracted solution of compression and spray drying.
Assuntos
Medicamentos de Ervas Chinesas/química , Plantas Medicinais/química , Tecnologia Farmacêutica/métodos , Dessecação , Medicamentos de Ervas Chinesas/isolamento & purificação , Temperatura , Viscosidade , Água/químicaRESUMO
By orthogonal design, and considering extracting efficiency and cost, optimizing the extract method of Gynostemma pentaphyllum polysaccharides. We purified the crude Gynostemma pentaphyllum polysaccharides initially, and assayed the polysaccharides content of Gynostemma pentaphyllum polysccharides. The content of Gynostemma pentaphyllum polysaccharides was sigificantly higher than the predecessor. It would provide conditions for the deep exploitation of Gynostemma pentaphyllum.