RESUMO
We have designed and developed novel pro-angiogenic bio-synthesized gold nanoconjugates (b-Au-HP) that make new blood vessels, as observed by several in vitro and in vivo assays, suggesting their future potential applications in alternative treatment strategies for wound healing, cardiovascular diseases (CVD) and ischemic diseases using a nanomedicine approach.
Assuntos
Ouro/farmacologia , Nanopartículas Metálicas , Nanoconjugados , Neovascularização Fisiológica/efeitos dos fármacos , Cicatrização/efeitos dos fármacos , Animais , Linhagem Celular , Linhagem Celular Tumoral , Sobrevivência Celular/efeitos dos fármacos , Embrião de Galinha , Cloretos/química , Células Endoteliais/efeitos dos fármacos , Células Endoteliais/fisiologia , Ouro/química , Compostos de Ouro/química , Química Verde , Hamelia , Humanos , Nanopartículas Metálicas/química , Nanoconjugados/química , Oxirredução , Extratos Vegetais/química , Folhas de Planta , Transdução de SinaisRESUMO
Thalidomide, a sedative drug given to pregnant women, unfortunately caused limb deformities in thousands of babies. Recently the drug was revived because of its therapeutic potential; however the search is still ongoing for an antidote against thalidomide induced limb deformities. In the current study we found that nitric oxide (NO) rescues thalidomide affected chick (Gallus gallus) and zebrafish (Danio rerio) embryos. This study confirms that NO reduced the number of thalidomide mediated limb deformities by 94% and 80% in chick and zebrafish embryos respectively. NO prevents limb deformities by promoting angiogenesis, reducing oxidative stress and inactivating caspase-3 dependent apoptosis. We conclude that NO secures angiogenesis in the thalidomide treated embryos to protect them from deformities.