Development of decellularized aortic scaffold for regenerative medicine using Sapindus mukorossi fruit pericarp extract.

The aim of this study is to develop a novel decellularization method using aqueous extract of soap nut pericarp (SPE) and its evaluation using hematoxylin-eosin staining, scanning electron microscopy, diamidino-2-phenylindol (DAPI) staining, mechanical testing, sodium dodecyl sulfate polyacrylamide gel electrophoresis and DNA quantification. The presently available decellularization agent raises some concerns due to the potential for presence of residual cytotoxic agents in the extracellular matrix. Histological analysis of hematoxylin and eosin and masson's trichrome stained processed aortic samples shows complete decellularization with preservation of extracellular matrix microarchitecture at 120 h. Further, staining of tissue samples with DAPI demonstrates complete removal of DNA fragments. Quantitative evaluation of DNA in the decellularized aorta tissues demonstrated a significant (P < 0.01) decrease in DNA content as compared to native tissues. Collagen quantification assay indicate no significant (P> 0.05) difference in its content between native and decellularized caprine aorta. Tensile strength of the decellularized scaffolds decreased non-significantly (P > 0.05) when compared to native tissues. There was no significant (P > 0.05) difference in young's modulus of elasticity, stiffness and stretch ratio between native aortic tissues and decellularized aortic scaffolds. Histological and scanning electron microscopic examination of in vitro cultured scaffold demonstrated the cell viability and proliferation of primary chicken embryo fibroblasts. SPE treatment is thus capable of producing cytocompatible decellularized caprine aorta scaffold with preservation of extracellular matrix architecture for vascular tissue engineering and could be applied widely as one of the decellularization agent.

Low-level laser therapy for management of large periapical lesions associated with open apex cases.

CONTEXT: Low-level laser therapy (LLLT) is an established method to accelerate wound healing through the phenomenon of biostimulation. AIMS: This case study presents a novel approach for management of open apex cases associated with large peripapical lesions using 980-nm diode laser for biostimulation. SETTINGS AND DESIGN: Case report. MATERIALS AND METHODS: Two patients presenting with open apex associated with periapical lesion were selected. After access opening, canal disinfection was carried out with sodium hypochlorite and 980-nm diode laser. Calcium hydroxide was given as intracanal medicament for 1 week. When patient was asymptomatic, apical plug of 4-5 mm MTA was placed. In addition, seven sessions of LLLT were administered transcutaneously around the apices of involved teeth every alternate day for 15 days. RESULTS: One year follow-up radiograph revealed remarkable healing of the lesion. CONCLUSION: The combination of increased cellular proliferation with laser biostimulation and meticulous canal disinfection with lasers has the potential of accelerating healing of the periapical lesions.

Cameo surface recording in complete denture fabrication using transcutaneous electrical nerve stimulation: A clinical report.

Severe bone loss in patients with complete edentulism poses a treatment challenge. In fabricating a denture, the stability of the prosthesis must be enhanced by recording the cameo surface within the confines of the physiological position of the cheek and tongue muscles (the neutral zone) and by shaping it accordingly. The treatment of a patient with a completely edentulous maxillary arch and severe maxillary anterior bone loss is described. The cameo surface was recorded within the physiological limits during the fabrication of a complete denture by using transcutaneous electrical nerve stimulation (TENS).

Treatment of oral submucous fibrosis with ErCr: YSGG laser.

Oral submucous fibrosis (OSMF) is one of the most poorly understood and unsatisfactorily treated diseases. Various medical and surgical treatments have been used but with limited benefits. However, with advent of lasers, oral surgeons are provided with new modality for treating OSMF. This case report highlights the pioneering effort in treating a moderate case of bilateral OSMF with Erbium Chromium Yttrium Scandium Gallium Garnet (ErCr:YSGG) laser showing promising result during follow-up.