Assuntos
Quimioterapia Combinada/uso terapêutico , Assistência de Longa Duração , Resistência a Meticilina , Minociclina/uso terapêutico , Rifampina/uso terapêutico , Infecções Estafilocócicas/prevenção & controle , Staphylococcus aureus/efeitos dos fármacos , Cateterismo/efeitos adversos , Humanos , Testes de Sensibilidade Microbiana , Cavidade Nasal/microbiologia , Projetos Piloto , Fatores de Risco , Infecções Estafilocócicas/microbiologia , Infecções Estafilocócicas/urinaRESUMO
A systematic study of pigment production (browning) and fluorescence (extracellular yellow-green and intracellular blue-white) by nine Legionellaceae species was performed. A total of 56 strains representing Tatlockia micdadei (Pittsburgh pneumonia agent), Legionella pneumophila, Legionella jordanis, Legionella longbeachae, Legionella oakridgensis, Legionella wadsworthii, Fluoribacter bozemanae, Fluoribacter gormanii, and Fluoribacter dumoffii could be separated on media supplemented with tyrosine plus cystine, 3,4-diaminobenzoic acid, 3,5-diaminobenzoic acid, and 3-aminotyrosine. Parallel testing by hippurate hydrolysis and the bromocresol purple spot test enabled the identification of Legionellaceae species 24 to 72 h after primary isolation. This schema may be a practical alternative to species-specific antisera methods (slide agglutination or direct immunofluorescence) in the identification of members of the family Legionellaceae.
Assuntos
Aminoácidos/metabolismo , Aminobenzoatos/metabolismo , Bactérias/classificação , Legionella/classificação , Pigmentos Biológicos/análise , Bactérias/metabolismo , Cistina/metabolismo , Fluorescência , Legionella/metabolismo , Tirosina/análogos & derivados , Tirosina/metabolismoRESUMO
In vitro susceptibilities of 53 clinical isolates of Pseudomonas aeruginosa to nine antipseudomonal antibiotics were determined. From 96 to 100% of the isolates were susceptible to piperacillin, ticarcillin, and 1-oxacephalosporin (LY 127935). Of the aminoglycosides, 89, 82, 79, and 29% were susceptible to amikacin, tobramycin, gentamicin, and netilmicin, respectively. A total of 96% and 78% of the isolates were susceptible to 1-oxacephalosporin (LY127935) and cefotaxime, respectively, at concentrations of 62.5 micrograms/l. Supplementation of testing media by calcium and magnesium not only markedly increased the minimal inhibitory concentrations of the aminoglycosides, but also raised those of cefotaxime and the penicillins; no significant effect was noted with 1-oxace-phalosporin. Synergy was not demonstrated consistently in vitro with 1-oxace-phalosporin combined with either carbenicillin, ticarcillin, gentamicin, or tobramycin.