RESUMO
Because of its capacity to rapidly convert superoxide to hydrogen peroxide, superoxide dismutase (SOD) is crucial in both intracellular signalling and regulation of oxidative stress. In this paper we report the cloning of a Cu/Zn SOD (designated as pfSOD) from the pearl oyster (Pinctada fucata) using rapid amplification of cDNA ends (RACE) PCR. The full-length cDNA of this Cu/Zn SOD contains an open reading frame (ORF) of 471 bp coding for 156 amino acids. No signal peptide was identified at the N-terminal amino acid sequence of Cu/Zn SOD indicating that this pfSOD encodes a cytoplasmic Cu/Zn SOD. This is supported by the presence of conserved amino acids required for binding copper and zinc. Semi-quantitative analysis in adult tissues showed that the pfSOD mRNA was abundantly expressed in haemocytes and gill and scarcely expressed in other tissues tested. After challenge with lipopolysaccharide (LPS), expression of pfSOD mRNA in haemocytes was increased, reaching the highest level at 8 h, then dropping to basal levels at 36 h. These results suggest that Cu/Zn SOD might be used as a bioindicator of the aquatic environmental pollution and cellular stress in pearl oyster.
Assuntos
Pinctada/genética , Superóxido Dismutase/genética , Sequência de Aminoácidos , Animais , Sequência de Bases , Clonagem Molecular , DNA Complementar/análise , DNA Complementar/genética , Lipopolissacarídeos/administração & dosagem , Dados de Sequência Molecular , Fases de Leitura Aberta , Especificidade de Órgãos , Filogenia , Pinctada/química , Pinctada/enzimologia , Pinctada/imunologia , RNA Mensageiro/análise , RNA Mensageiro/genética , Reação em Cadeia da Polimerase em Tempo Real/veterinária , Alinhamento de Sequência/veterinária , Superóxido Dismutase/química , Superóxido Dismutase/imunologia , Superóxido Dismutase/metabolismoRESUMO
A polysaccharide isolated and purified from Tamarindus indica shows immunomodulatory activities such as phagocytic enhancement, leukocyte migration inhibition and inhibition of cell proliferation. These properties suggest that this polysaccharide from T. indica may have some biological applications.
Assuntos
Adjuvantes Imunológicos/farmacologia , Plantas Medicinais/química , Polissacarídeos/farmacologia , Adjuvantes Imunológicos/isolamento & purificação , Divisão Celular/efeitos dos fármacos , Quimiotaxia de Leucócito/efeitos dos fármacos , Humanos , Hidrólise , Técnicas In Vitro , Índia , Linfócitos/efeitos dos fármacos , Fagocitose/efeitos dos fármacos , Polissacarídeos/isolamento & purificação , Leucemia-Linfoma Linfoblástico de Células Precursoras/fisiopatologia , Células Tumorais CultivadasRESUMO
Lectins from the seeds of Jackfruit (Artocarpus integrifolia) and winged bean (Psophocarpus tetragonolobus) were isolated using an immobilized N-acetyl D-galactosamine column and conjugated to type VI horse radish peroxidase. The purified conjugate was used for the study of tissue specificities using diaminobenzidine as the substrate on dewaxed tissue sections of normal, oral leukoplakia, oral submucous fibrosis, verucous carcinoma and squamous cell carcinoma of the oral cavity. In spite of having a common inhibitory sugar, winged bean lectin did not bind to any lectins whereas Jackfruit lectin showed varying degrees of binding towards the above tissues. The difference in the nature and intensity of binding of the Jackfruit lectin suggest the utilizing this lectin in the differential diagnosis of the premalignant and malignant lesions of the oral cavity.