RESUMO
Type 2 diabetes mellitus (T2DM) has been regarded as a critical risk factor for pulmonary tuberculosis (PTB). Ginsenoside Rg1 has been identified as a potential therapeutic agent for T2DM by suppressing the inflammatory response. However, the effect of Rg1 on T2DM-associated PTB has not been reported. In this study, we aimed to explore the function of Rg1 in the regulation of T2DM-associated PTB. We established a T2DM-associated PTB mouse model and found that the fibrosis of lung tissues was inhibited by Rg1 in T2DM-associated PTB mice. The lung injury of T2DM-associated PTB mice was repressed by Rg1. Moreover, the levels of IL-6, TNF-α, and IL-1ß in the lung tissues and serum were decreased by Rg1 in T2DM-associated PTB mice. The treatment with Rg1 inhibited the levels of free fatty acid and enhanced the expression of miR-15b-5p in lung tissues of T2DM-associated PTB mice. MiR-15b-5p targeted and inhibited the STAT3 expression. The expression of STAT3 was downregulated by Rg1, while the inhibition of miR-15b-5p reversed the downregulation. The expression of miR-15b-5p was reduced, but the expression of STAT3 was upregulated in the lung tissues of T2DM-associated PTB mice. We validated that miR-15b-5p attenuated inflammation and lung injury in the T2DM-associated PTB mouse model. The overexpression of STAT3 or the suppression of miR-15b-5p restored lung fibrosis and injury inhibited by Rg1 in T2DM-associated PTB mice. Meanwhile, the Rg1-repressed levels of IL-6, TNF-α, and IL-1ß were enhanced by the overexpression of STAT3 or the suppression of miR-15b-5p. In addition, the levels of free fatty acid repressed by Rg1 were reversed by STAT3 overexpression and miR-15b-5p inhibition. Thus, we conclude that ginsenoside Rg1 inhibits the STAT3 expression by miR-15b-5p to attenuate lung injury in mice with type 2 diabetes mellitus-associated pulmonary tuberculosis.
RESUMO
Two new glycosides, namely panalaside A (1) and panalaside B (2) were isolated from from Panzerina lanata (L.) Soják. The structures of 1 and 2 were elucidated by spectroscopic methods, including UV, IR, HR-ESI-MS and extensive 1 D and 2 D NMR techniques.
Assuntos
Medicamentos de Ervas Chinesas , Lamiaceae , Medicamentos de Ervas Chinesas/química , Glicosídeos/química , Estrutura MolecularRESUMO
A new alkaloid, named as panalasin (1), was isolated from the ethyl acetate extract from Panzerina lanata (L.) Soják. The structure of 1 was elucidated by spectroscopic methods, including UV, IR, HR-ESI-MS and extensive 1D and 2D NMR techniques.
Assuntos
Alcaloides/química , Lamiaceae/química , Acetatos/química , Espectroscopia de Ressonância Magnética , Estrutura Molecular , Extratos Vegetais/química , Espectrometria de Massas por Ionização por Electrospray , Espectrofotometria UltravioletaRESUMO
A new sesquiterpene, artefrigin (5), together with four known sesquiterpenes were isolated from the volatile oil of Artemisia frigida Willd. The structure of five was elucidated by spectroscopic methods, including UV, IR, HR-ESI-MS and extensive 1D and 2D NMR techniques.
Assuntos
Artemisia/química , Óleos Voláteis/química , Espectroscopia de Prótons por Ressonância Magnética , Sesquiterpenos/químicaRESUMO
Two new compounds, namely integrin A (1) and integrin B (2), were isolated from the supercritical fluid extract (SFE) of Artemisia integrifolia L. Their structures were elucidated by spectroscopic methods, including UV, IR, HR-ESI-MS and extensive 1D and 2D NMR techniques.
Assuntos
Artemisia/química , Extratos Vegetais/química , Espectroscopia de Ressonância Magnética Nuclear de Carbono-13 , Espectroscopia de Prótons por Ressonância MagnéticaRESUMO
Sacroflavonoside, a new derivative of diphenylethene, was isolated from Artemisia sacrorum, which have been found to possess the inhibitory effect on the proliferation of gastric carcinoma cells (MKN-45) in vitro in our previous studies. With anisaldehyde (SM-A) as starting material, the sacroflavonoside was synthesized by nucleophilic addition, electrophilic substitution and dehydration cyclization. The structure of sacroflavonoside was established by 1 D (1H NMR and 13C NMR) and 2 D-NMR (HSQC and HMBC) spectral analysis. The antitumor activity and potential mechanism against MKN-45 cells of sacroflavonoside were evaluated in vitro. The results showed that sacroflavonoside could significantly induce MKN-45 cells apoptosis and autophagy by increasing the expression of Bax, Caspase-3, Beclin1 and LC3-II proteins and decreasing the expression of Bcl-2 protein at low micromole level. This investigation provided a valuable lead structure for the development of antitumor drugs.
Assuntos
Antineoplásicos Fitogênicos/síntese química , Antineoplásicos Fitogênicos/farmacologia , Apoptose/efeitos dos fármacos , Artemisia/química , Autofagia/efeitos dos fármacos , Proteína Beclina-1/metabolismo , Benzaldeídos/química , Caspase 3/metabolismo , Linhagem Celular Tumoral , Ciclização , Humanos , Espectroscopia de Ressonância Magnética , Estrutura Molecular , Proteínas Proto-Oncogênicas c-bcl-2/metabolismo , Neoplasias Gástricas/tratamento farmacológico , Neoplasias Gástricas/metabolismo , Neoplasias Gástricas/patologiaRESUMO
Two new compounds, namely arteordoyn A (1) and arteordoyn B (2), together with four known compounds, were isolated from the petroleum ether extract of Artemisia ordosica Krasch. The structures elucidation of 1 and 2 were carried out by 1D-NMR (1H and 13C NMR), 2D-NMR (COSY, HSQC, HMBC and NOESY) and HR-ESI-MS spectral analysis.
Assuntos
Artemisia/química , Extratos Vegetais/química , Espectroscopia de Ressonância Magnética , Estrutura Molecular , Polímero Poliacetilênico/química , Polímero Poliacetilênico/isolamento & purificação , Espectrometria de Massas por Ionização por ElectrosprayRESUMO
A new dihydrochalcone, namely 2',5'-dimethyl-3'-methoxy-4',6'-dihydroxyl-dihydrochalcone (1) together with five known compounds were isolated from the CHCl3 extract from Empetrum nigrum L. var. japonicum K. Koch (E. nigrum). The structures of 1 was elucidated by spectroscopic methods, including UV, IR, HR-ESI-MS and extensive 1D and 2D NMR techniques.
Assuntos
Chalconas/isolamento & purificação , Ericaceae/química , Chalconas/química , Espectroscopia de Ressonância Magnética , Estrutura Molecular , Extratos Vegetais/química , Análise EspectralRESUMO
Two new compounds, named ordosacid A (5) and ordosacid B (6), along with four known compounds: 3,4-dihydroxybenzaldehyde (1), p-hydroxybenzoic acid (2), p-hydroxycinnamic acid (3) and o-hydroxycinnamic acid (4), were isolated from the ethyl acetate extract of Artemisia ordosica Krasch. The structures of new compounds were elucidated on the basis of spectroscopic methods including UV, IR, ESI-MS, 1D NMR, 2D NMR, HR-ESI-MS and modified Mosher's method.
Assuntos
Artemisia/química , Produtos Biológicos/química , Artemisia/metabolismo , Produtos Biológicos/isolamento & purificação , China , Ácidos Cumáricos , Espectroscopia de Ressonância Magnética , Estrutura Molecular , Parabenos/química , Parabenos/isolamento & purificação , Extratos Vegetais/química , Propionatos/química , Propionatos/isolamento & purificação , Metabolismo Secundário , Espectrometria de Massas por Ionização por Electrospray , Espectrofotometria UltravioletaRESUMO
A new compound, integracid (1), together with four known compounds were isolated from the dichloromethane (CH2Cl2) extract from Artemisia integrifolia L. The structures of compounds (1-5) were elucidated by spectroscopic methods, including ultraviolet, infrared (IR), high resolution-electrospray ionization-mass spectrometry (HR-ESI-MS) and extensive one-dimensional (1D) and two-dimensional (2D) nuclear magnetic resonance (NMR) techniques, and by comparison with data reported in the references. Antibacterial activities of the compounds were evaluated against various bacteria.
Assuntos
Anti-Infecciosos/química , Artemisia/química , Extratos Vegetais/química , Anti-Infecciosos/farmacologia , Bacillus cereus/efeitos dos fármacos , Extratos Vegetais/farmacologia , Staphylococcus aureus/efeitos dos fármacos , Triterpenos/química , Triterpenos/farmacologia , Yersinia enterocolitica/efeitos dos fármacosRESUMO
Artemisia integrifolia L (Compositae) is a medicinal and edible plant. To investigate its antihyperlipidemic effect, a crude lipophilic extract and the composing compounds were isolated and fractioned from the petroleum ether extract of aerial parts of A. integrifolia using column chromatography on silica gel. The anti-hyperlipidemia effect was studied in a rat model of acute hyperlipidemia, which was induced by triton WR-1339. A new compound, integrinol (4), together with nine known compounds, namely chamazulene (1), acetylenes (E)-2 (2), acetylenes (E)-3 (3), eugenol (5), palmitic acid (6), oleic acid (7), linoleic acid (8), linolenic acid (9) and 12,13-epoxylinolenic acid were isolated from the crude lipophilic extract of A. integrifolia. The LD50 value of the crude extract was more than 4g/kg. In Triton WR-1339-induced acute hyperlipidemia model, the crude lipophilic extract (200 mg/kg) significantly reduced total cholesterol (TC) by 70% (p ≤ 0.01) and triglycerides (TGs) by 94% (p ≤ 0.001). The fractioned compounds, such as chamazulene (1), acetylene-2 (2), and linolenic acid (9), used at 4 mg/kg dose, also significantly decreased the concentrations of TC (32%, 33% and 64%, respectively) and TGs (48%, 33% and 93%, respectively). These compounds (i.e., chamazulene, acetylenes (E)-2, and linolenic acid) were considered to be responsible for the bioactive antihyperlipidemic effect. In conclusion, the crude lipid extract of Artemisia integrifolia L could be used as a potential treatment to avert hyperlipidemia. Further studies to confirm these results in other models of hyperlipidemia (e.g., diet-induced obesity) are warranted.
Assuntos
Artemisia/química , Interações Hidrofóbicas e Hidrofílicas , Hipolipemiantes/química , Hipolipemiantes/farmacologia , Fracionamento Químico , Hipolipemiantes/isolamento & purificação , Espectroscopia de Ressonância Magnética , Estrutura Molecular , Compostos Fitoquímicos/química , Extratos Vegetais/química , Extratos Vegetais/isolamento & purificação , Extratos Vegetais/farmacologiaRESUMO
A new triterpenoid, namely 3ß-hydroxy-13ß,28-epoxyolean-11-enyl trans-caffeate (1), was isolated from the EtOAc extract from Ixeris chinensis Nakai. The structures of 1 were elucidated by spectroscopic methods, including UV, IR, HR-ESI-MS and extensive 1D and 2D NMR techniques.
Assuntos
Asteraceae/química , Espectroscopia de Ressonância Magnética , Triterpenos/química , Acetatos , Estrutura Molecular , Extratos Vegetais/química , Análise Espectral , Triterpenos/isolamento & purificaçãoRESUMO
A new alkaloid, 2-amino-1,6-dihydro-pyridine-5-carbaldehyde (1), together with four known compounds, namely 4-hydroxybenzaldehyde (2), 4-hydroxyacetophenone (3), acetophenone-4-O-ß-d-glucoside (4), 2-hydroxyl-6-methoxyacetophenone-4-O-ß-d-glucoside (5), were isolated from the CHCl3 extract from Ixeris chinensis Nakai. The structures of 1 was elucidated by spectroscopic methods, including UV, IR, HR-ESI-MS and extensive 1D and 2D NMR techniques.
Assuntos
Aldeídos/química , Alcaloides/química , Asteraceae/química , Piridinas/química , Etanol , Espectroscopia de Ressonância Magnética , Extratos Vegetais/química , Espectrometria de Massas por Ionização por Electrospray , Espectrofotometria Infravermelho , Espectrofotometria UltravioletaRESUMO
Two new compounds, named as sacric acid A (1) and sacric acid B (2), were isolated from the EtOAc extract of Artemisia sacrorum Ledeb. This is the first report on the structure elucidation of these compounds based on UV, IR, and extensive ID and 2D NMR spectroscopic, and ESI-MS techniques.
Assuntos
Artemisia/química , Ácidos Cafeicos/isolamento & purificação , Ácidos Cafeicos/química , Estrutura MolecularRESUMO
A new lignan, 4,4'-dihydroxy-3,3'-dimethoxy-6,9'-cycloligna-7,8'-dien-9'-al, was isolated from the stem of Syringa pinnatifolia Hems1. var. alashanensis MA. et S.Q. Zhou (S. pinnatifolia). This is the first report on the structure elucidation of a new compound based on spectroscopic methods including UV, IR, ESI-MS, 1D NMR and 2D NMR techniques.
Assuntos
Naftóis/química , Syringa , Lignanas/química , Espectroscopia de Ressonância Magnética , Estrutura Molecular , Extratos Vegetais/química , Caules de Planta/química , Espectrometria de Massas por Ionização por Electrospray , Espectrofotometria Infravermelho , Espectrofotometria Ultravioleta , Syringa/químicaRESUMO
The aerial parts of Artemisia frigida Willd. are used to treat joint swelling, renal heat, abnormal menstruation, and sore carbuncle. The anti-inflammatory effects of A. frigida have been well-known in folk medicine, suggesting that components extracted from A. frigida could potentially treat inflammatory disease. With the aim of discovering bioactive compounds, in this study, we extracted total flavonoids from the aerial parts of A. frigida and investigated their anti-inflammatory effects against inflammation induced by carrageenan and egg albumin in rats. At the doses studied, total flavonoids (100 mg/kg, 200 mg/kg, and 400 mg/kg) and some isolated compounds (30 mg/kg) showed significant and dose-dependent anti-inflammatory effects. According to the high-performance liquid chromatography analysis of the total flavonoids from A. frigida, there are five major compounds, namely, 5-hydroxy-3',4'-dimethoxy-7-O-ß-d-glucuronide (F1), 5-hydroxy-3',4',5'-trimethoxy-7-O-ß-d-glucuronide (F2), 5,7,3'-trihydroxy-6,4'-dimethoxyflavone (F3), 5,3'-dihydroxy-6,7,4'-trimethoxyflavone (F4), and 5,3'-dihydroxy-3,6,7,4'-tetramethoxyflavone (F5), which may explain the anti-inflammatory activity.
Assuntos
Artemisia , Animais , Anti-Inflamatórios , Cromatografia Líquida de Alta Pressão , Flavonoides , Espectroscopia de Ressonância Magnética , Extratos Vegetais , RatosRESUMO
The present study was designed to isolate and characterize the analgesic compounds of Artemisa sacrorum Ledeb. The EtOAc crude extracts from the aerial parts of Artemisa sacrorum Ledeb were separated by chromatography and the structures of new compounds were elucidated based on spectral analyses. Analgesic activities of the isolated compounds were assessed in rats with hot plate test and paw pressure assay. Two new flavone C-glycosides, named as Sacroroside A and B (Compounds 1 and 2) were isolated from the EtOAc crude extract of the aerial parts ofArtemisa sacrorum Ledeb. They showed significant analgesic effects. In conclusion, Compounds 1 and 2 are new natural products, which show significant analgesic effects in a dose-dependent manner.
Assuntos
Analgésicos/uso terapêutico , Artemisia/química , Dissacarídeos/uso terapêutico , Flavanonas/uso terapêutico , Flavonas/uso terapêutico , Dor/tratamento farmacológico , Fitoterapia , Extratos Vegetais/uso terapêutico , Analgésicos/química , Analgésicos/isolamento & purificação , Analgésicos/farmacologia , Animais , Dissacarídeos/química , Dissacarídeos/isolamento & purificação , Dissacarídeos/farmacologia , Flavanonas/química , Flavanonas/isolamento & purificação , Flavanonas/farmacologia , Flavonas/química , Flavonas/isolamento & purificação , Flavonas/farmacologia , Temperatura Alta , Masculino , Estrutura Molecular , Componentes Aéreos da Planta , Extratos Vegetais/química , Extratos Vegetais/farmacologia , Pressão , Ratos WistarRESUMO
Two new compounds, namely 5-methyl-4,2',3',5'-tetrahydroxy-4'-methoxy-2-aldehyde biphenyl (1) and 1,8-dioxo-1,8-dihydropyrano[3,4-c]pyran-3,6- dicarboxyl acid, diethyl ester (2), were isolated from the 95% ethanol extract of the roots of Paeonia lactiflora Pall. The structures of 1 and 2 were elucidated by spectroscopic methods, including UV, IR, HR-ESI-MS and extensive 1D and 2D NMR techniques.
Assuntos
Compostos de Bifenilo/química , Paeonia/química , Piranos/química , Estrutura Molecular , Raízes de Plantas/químicaRESUMO
Three biflavonoid glycosides, named carinoside B, C and D (1, 2 and 3, respectively), were isolated from the n-BuOH crude extract of the aerial part of Lomatogonium carinthiacum (Wulfen) Rchb (L. carinthiacum). The structures of 1, 2 and 3 were elucidated by spectroscopic methods, including UV, IR, HR-ESI-MS and extensive 1D and 2D NMR techniques.