[Effects of leaf extracts of Amorpha fruticosa on seed germination and seedling growth of Amygdalus pedunculata]. / 紫穗槐叶片浸提液对长柄扁桃种子萌发和幼苗生长的影响.

Amorpha fruticosa and Amygdalus pedunculata are common plant species used for greening construction in arid and semi-arid region of Northwest China. In order to explore the feasibility of greening construction and ecological restoration by A. fruticose with A. pedunculata, we exami-ned the allelopathic effects of five concentrations of aqueous leaf extracts of A. fruticosa (0.025, 0.05, 0.10, 0.15 and 0.20 g·mL-1) on eight A. pedunculata varieties (YY1, YY3, YY4, YY5, YY6, SM6, SM7 and SM8), using the methods of paper-petri dish and soilless culture. The results showed that when the concentration of A. fruticosa leaf extracts were 0.025 and 0.05 g·mL-1, the seed germination and seedling growth of YY1 and SM6 were significantly better than other varieties. With increasing concentration of A. fruticosa leaf extracts, the catalase activity of A. pedunculata seedlings first increased and then decreased. The activities of peroxidase and superoxide dismutase, and the contents of soluble protein and chlorophyll showed a downward trend, while the contents of malondialdehyde and soluble sugar and the permeability of cell membrane gradually increased. Results of the principal component and cluster analysis showed that the growth potential of A. pedunculata decreased with the order of YY1, SM6, SM8, SM7, YY6, YY3, YY5 and YY4 under the allelopathic effect of A. fruticose. In conclusion, the artificial collocation and mixed planting of low-density of A. fruticosa with YY1 and SM6 were beneficial to seed germination and seedling growth of A. pedunculata.

[Rheology and in vitro release properties of thermosensitive in situ gel of Yihuang Decoction and its common gel for vaginal use].

To evaluate the traits and rheological properties of thermosensitive in situ gel of Yihuang Decoction and its common gel for vaginal use, and predict the release behavior of Yihuang Decoction in situ gel in vitro. Poloxamer was used as thermosensitive material to prepare Yihuang Decoction vaginal in situ gel, and Yihuang Decoction common gel was prepared with carbopol. Then the differences of the two gels before and after diluting with vaginal fluid were compared. The rheological parameters of Yihuang Decoction in situ gel and its common gel were determined with Anton Paar MCR102 rheometer. In addition, berberine hydrochloride was selected as an index component to evaluate the in vitro release properties of Yihuang Decoction vaginal thermosensitive in situ gel. Yihuang Decoction vaginal thermosensitive in situ gel was Newtonian fluid under low-temperature conditions, which was yellow and transparent. After reaching the gelling temperature of 24.5 ℃, it became semi-solid, pseudoplastic fluid. The gelling temperature was predicted to be 37 ℃, and the phase transition time was 30 s after diluting with simulated vaginal fluid. However, the rheological properties of Yihuang Decoction common gel had no significant changes with temperature. Compared with in situ gel, the color of common gel was darker and more translucent. Besides, its mobility was stronger after diluting with simulated vaginal fluid. The in vitro release study showed that the kinetic behavior of berberine hydrochloride in Yihuang Decoction vaginal thermosensitive in situ gel was matched with the Higuchi equation. Through simulation of vaginal administration, physical properties and dynamic rheological parameters were used to intuitively and scientifically evaluate the two gels. Compared with the common gel, the thermosensitive in situ gel could quickly attached to the vaginal mucosa and release drug, and thus was more suitable for developing vaginal administration of Yihuang Decoction, which also provides references for studying new vaginal preparation of Yihuang Decoction.