RESUMO
Objective To detect the expression levels of peripheral blood CXCL10 and its receptor CXCR3 and T cell subsets in of the patients with advanced vitiligo and the influence of compound Chinese medicine on it.Methods Flow cytometry was used to detect the cellular proportions of peripheral blood T cell subsets,ELISA was employed to quantify serum CXCL10 and CXCR3 expression levels before and after treatment.Results After 1 month of taking Chinese medicine,the proportions of CD3+ CD4+ cells and CD3+ CD8+ cells were increased compared before treatment(P＜0.05).The expression level of peripheral serum CXCL10 before treatment was significantly increased compare with the healthy control group(P＜0.01),and the CXCL10 level after treatment was decreased significantly compared with that before treatment(P＜0.05).The expression level of peripheral serum CXCR3 was significantly increased compared with the healthy control group(P＜0.05),while which after treatment was still significantly higher than that in the healthy control group(P＜0.05).Conclusion CXCL10,CXCR3 and T cell subsets proportion may be involved in the pathogenesis of vitiligo.The compound Chinese medicine used in this study plays the curative effect possibly by regulating T cell subsets and expression levels of CXCL10 and CXCR3.
RESUMO
Objective To observe the curative effects of two traditional Chinese medicine compounds in guinea pigs models of vitiligo .Methods The dark skin of guinea pigs ,which were intragastrically administrated with vitiligo granules No .1 (No .1 group) ,vitiligo granules No .2(No .2 group) and pure water(control group) respectively ,were selected to establish vitiligo modles induced by p‐dihydroxy‐benzene(hydroquinone) .Taking physiological saline as a control(model group) .Curative effects were ob‐served by visual inspection ,and the effects of repigmentation after intragastric administration were scored .The melanocyte count of normal part of skin was compared with that of vitiligo skin by using hematoxylin‐eosin(HE) staining ,ferrous sulfate staining and Deoxy‐dyeing .Results The guinea pigs models of vitiligo were successfully induced by hydroquinone .After treatment by using vit‐iligo granules No .1 and No .2 ,the melanocyte counts of vitiligo skin in No .1 group and No .2 group were increased when compared with that in the control group(P0 .05) .Conclusion The two Chinese medicine compounds ,vitiligo granules No .1 and vitiligo granules No .2 ,both are effective for the treatment of vitiligo and without obvious effects on normal parts of skin , which could provide experimental data for clinical treatment .