RESUMO
Various bioactive substances in kiwifruit extracts were fractionated by organic solvent extractions, followed by silica gel and ODS chromatographies. Both cytotoxic activity and multi-drug resistance reversal activity were found in the less polar fractions. Cytotoxic activity was not always parallel the radical intensity. Antibacterial activity was distributed into various fractions and all fractions were inactive against Candida albicans and H. pylori. Only 70% methanol extracts showed anti-human immunodeficiency virus activity, and produced a broad ESR signal under alkaline conditions, in a fashion similar to lignin. These fractions also effectively scavenged O(2)(-) produced by the xanthine-xanthine oxidase reaction, suggesting a bimodal (pro-oxidant and antioxidant) action. These data suggest a medicinal efficacy of kiwifruit peel extracts.
Assuntos
Anti-Infecciosos/farmacologia , Candida albicans/efeitos dos fármacos , Sequestradores de Radicais Livres/farmacologia , HIV-1/efeitos dos fármacos , Helicobacter pylori/efeitos dos fármacos , Magnoliopsida , Plantas Medicinais , Animais , Antibacterianos , Resistência a Múltiplos Medicamentos , Frutas , Humanos , Camundongos , Testes de Sensibilidade Microbiana , Extratos Vegetais/farmacologia , Células Tumorais Cultivadas/efeitos dos fármacosRESUMO
Allium victorialis L. (Liliaceae, "Hon-Gyoujya Nin-Niku" in Japanese) was successively extracted with hexane, acetone, methanol and 70% methanol and the extracts were further separated into a total of twenty-five fractions by silica gel and ODS column chromatographies. The biological activities of these four extracts and 25 column fractions were compared. The cytotoxic activity of all extracts and fractions against two oral tumor cell lines was significantly higher than that against normal human gingival fibroblasts, suggesting their tumor-specific action. Three methanol column fractions [M2, M3, M6] and a 70% methanol column fraction [70M6] most effectively reversed the multidrug resistance (MDR) against L5178 mouse T cell lymphoma. The electron spin resonance (ESR) spectroscopy showed that methanol column fractions and 70% methanol extracts produced the highest amount of radical(s) and most efficiently scavenging O2*-, generated by the hypoxanthine-xanthine reaction system, suggesting that the same substances in these fractions display both prooxidant and antioxidant properties. They showed no anti-human immunodeficiency virus (HIV) or anti-Helicobacterpylori activity. These data suggest the medicinal efficacy of Allium victorialis extract.
Assuntos
Allium/química , Antioxidantes/farmacologia , Extratos Vegetais/farmacologia , Animais , Antibacterianos , Fármacos Anti-HIV/química , Fármacos Anti-HIV/farmacologia , Anti-Infecciosos/farmacologia , Antineoplásicos/farmacologia , Antioxidantes/química , Carcinoma de Células Escamosas/tratamento farmacológico , Resistência a Múltiplos Medicamentos , Ensaios de Seleção de Medicamentos Antitumorais , Espectroscopia de Ressonância de Spin Eletrônica , Sequestradores de Radicais Livres/farmacologia , Helicobacter pylori/efeitos dos fármacos , Humanos , Leucemia de Células T/tratamento farmacológico , Linfoma de Células T/tratamento farmacológico , Camundongos , Testes de Sensibilidade Microbiana , Extratos Vegetais/química , Superóxidos/químicaRESUMO
Fractionated extracts of Feijoa peels were studied for cytotoxic activity, anti-human immunodeficiency virus (HIV) activity and antibacterial activity. Two most cytotoxic fractions A3 of acetone extract and M2 of methanol extract had potent inhibitory activity against Gram-positive and Gram-negative bacteria as well as fungi tested. Fraction A4 of acetone extract showed multidrug resistance (MDR)-reversal activity comparable with that of verapamil (positive control). These results indicate the therapeutic value of Feijoa peel extracts as potential antimicrobial and MDR-modulating agents.