Preparation of the sphingolipid fraction from mycelia of Cordyceps sinensis and its immunosuppressive activity.

ETHNOPHARMACOLOGICAL RELEVANCE: Cordyceps sinensis (CS) is an herbal tonic in traditional Chinese medicine and is used to treat a wide range of disorders, including immune, kidney, respiratory, lung and cardiovascular diseases, in China. Most studies are focused mainly on nucleotides and polysaccharides from CS and consider them to be the main active ingredients, while other ingredients are often disregarded. Hundreds of sphingolipids have been identified from CS and showed inhibitory effects on mouse splenic lymphocytes. AIM OF THE STUDY: This study aimed to establish a method for preparing a fraction of sphingolipids from the mycelial powder of CS and evaluate its immunosuppressive activity. MATERIALS AND METHODS: Fraction of sphingolipids (Fr-SPLs) were prepared by silica gel chromatography and reversed-phase chromatography. Its components were identified and quantified by Quadrupole-Orbitrap UHPLC-MS/MS. PBMCs were prepared from human blood, and splenic lymphocytes, B cells, and T cells were prepared from mouse spleens. The inhibitory effect of Fr-SPLs on cell viability was evaluated by CCK-8 assay. PBMC apoptosis and the ratio of CD4+ T cells and CD8+ T cells were quantified by flow cytometry analysis. The expression of IL-2, IL-10, and TNF-α in PBMCs was detected by ELISA kits. RESULTS: A fraction containing 84.83% of sphingolipids (SPLs) was prepared from the mycelia of CS and named Fr-SPLs. 15 SPLs were identified from the Fr-SPLs. Fr-SPLs significantly inhibited the viability of human peripheral blood mononuclear cells (PBMCs) with an IC50 value of 9.82 µg/mL and promoted PBMC apoptosis in a dose-dependent manner. Moreover, Fr-SPLs inhibited the viability of mouse splenocytes, as well as that of B cells and T cells derived from splenocytes. Furthermore, Fr-SPLs reduced the production of IL-2, IL-10, and TNF-α in PBMCs. CONCLUSIONS: Fr-SPLs show immunosuppressive activity, and this study will be useful for preparing immunosuppressive components from CS and its mycelia for hyperimmune disease.

Discrimination between Alternative Herbal Medicines from Different Categories with the Electronic Nose.

As alternative herbal medicine gains soar in popularity around the world, it is necessary to apply a fast and convenient means for classifying and evaluating herbal medicines. In this work, an electronic nose system with seven classification algorithms is used to discriminate between 12 categories of herbal medicines. The results show that these herbal medicines can be successfully classified, with support vector machine (SVM) and linear discriminant analysis (LDA) outperforming other algorithms in terms of accuracy. When principal component analysis (PCA) is used to lower the number of dimensions, the time cost for classification can be reduced while the data is visualized. Afterwards, conformal predictions based on 1NN (1-Nearest Neighbor) and 3NN (3-Nearest Neighbor) (CP-1NN and CP-3NN) are introduced. CP-1NN and CP-3NN provide additional, yet significant and reliable, information by giving the confidence and credibility associated with each prediction without sacrificing of accuracy. This research provides insight into the construction of a herbal medicine flavor library and gives methods and reference for future works.