RESUMO
As a folk medicine, Moringa oleifera L. is used effectively to treat inflammatory conditions and skin diseases. However, its mechanism of action is not well understood, limiting its medical use. We isolated and identified three compounds, namely niazirin, marumoside A and sitosterol-3-O-ß-d-glucoside, from the seeds of Moringa oleifera, and studied their effects on the expression of Th17-relevant cytokines (IL-12/IL-23 p40, IL-17A, IL-22 and IL-23 p19) using lipopolysaccharide-stimulated THP-1 cells. Additionally, as Th17 plays a critical role in the pathogenesis of psoriasis, we used a 12-O-tetradecanoylphorbol-13-acetate (TPA)-induced psoriasis-like skin lesion mouse model to study their potential therapeutic application in vivo. The compounds suppressed the expression of IL-12/IL-23 p40, IL-17A, IL-22 and IL-23 p19 in vitro, and in vivo they ameliorated psoriasis-like skin lesions, decreased IL-17A mRNA expression, and increased the expression of keratinocyte differentiation markers. To our knowledge, this is the first report regarding the mechanism and therapeutic application of Moringa oleifera seeds to treat psoriasis-like lesions in vivo.
Assuntos
Citocinas/genética , Moringa oleifera/química , Extratos Vegetais/administração & dosagem , Psoríase/tratamento farmacológico , Acetato de Tetradecanoilforbol/efeitos adversos , Células Th17/imunologia , Animais , Modelos Animais de Doenças , Regulação da Expressão Gênica/efeitos dos fármacos , Glicosídeos/administração & dosagem , Glicosídeos/isolamento & purificação , Glicosídeos/farmacologia , Humanos , Lipopolissacarídeos/efeitos adversos , Camundongos , Estrutura Molecular , Extratos Vegetais/química , Extratos Vegetais/farmacologia , Psoríase/induzido quimicamente , Psoríase/genética , Pirróis/administração & dosagem , Pirróis/isolamento & purificação , Pirróis/farmacologia , Sementes/química , Sitosteroides/administração & dosagem , Sitosteroides/isolamento & purificação , Sitosteroides/farmacologia , Células Th17/efeitos dos fármacosRESUMO
The antimicrobial activities of longan (Dimocarpus longan Lour. Fen ke) seed extracts were investigated using a disc diffusion method and also determining the minimal inhibitory concentration. The DL-P01-SI01 fraction showed that the strongest activity against Staphylococcus aureus and methicillin-resistant S. aureus at MIC 64 µg/mL, which was found to be due to the phenolic compounds. The HPLC analysis showed that the major phenolic compounds are gallic acid, corilagin, ethyl gallate and ellagic acid.