RESUMO
A new triterpenoid saponin, clematomandshurica saponin E, together with four known saponins were isolated and characterized from the roots and rhizomes of Clematis mandshurica (Ranunculaceae), a commonly used traditional Chinese medicine with anti-inflammatory and antirheumatoid activities. On the basis of spectroscopic analysis, including HR-ESI-MS, IR, 1D, and 2D NMR spectral data and hydrolysis followed by chromatographic analysis, the structure of the new triterpenoid saponin was elucidated as 3-O-α-L-rhamnopyranosyl-(1 â 6)-ß-D-glucopyranosyl-(1 â 4)-ß-D-glucopyranosyl-(1 â 4)-ß-D-ribopyranosyl-(1 â 3)-α-L-rhamnopyranosyl-(1 â 2)-α-L-arabinopyranosyl oleanolic acid 28-O-ß-D-glucopyranosyl-(1 â 6)-ß-D-glucopyranoside.
Assuntos
Clematis/química , Medicamentos de Ervas Chinesas/isolamento & purificação , Plantas Medicinais/química , Saponinas/isolamento & purificação , Triterpenos/isolamento & purificação , Medicamentos de Ervas Chinesas/química , Estrutura Molecular , Ressonância Magnética Nuclear Biomolecular , Raízes de Plantas/química , Saponinas/química , Estereoisomerismo , Triterpenos/químicaAssuntos
Ginsenosídeos/farmacologia , Hepatócitos/efeitos dos fármacos , Cirrose Hepática Experimental/metabolismo , Inibidor Tecidual de Metaloproteinase-1/metabolismo , Animais , Feminino , Ginsenosídeos/uso terapêutico , Hepatócitos/metabolismo , Hepatócitos/ultraestrutura , Cirrose Hepática Experimental/tratamento farmacológico , Masculino , Fitoterapia , RNA Mensageiro/genética , Ratos , Ratos Sprague-DawleyRESUMO
OBJECTIVE: To explore the effect of low-energy semiconductor laser intranasal irradiation of the blood on blood coagulation status in healthy pregnant women at term. METHODS: Low-energy semiconductor laser was introduced into the nasal cavity in 126 healthy pregnant women at term and 123 healthy young unmarried women as the control group. The plasma prothrombin time (PT), activated partial thromboplastin time (APTT), thrombin time (TT), and fibrinogen levels were examined using transmissive turbidimetry after the therapy. RESULTS: PT, APTT and TT levels were significantly lowered, whereas fibrinogen level significantly increased in the healthy pregnant women before the laser therapy as compared with those in the control group (P<0.01). After intranasal laser therapy, these parameters were significantly improved in the healthy pregnant women (P<0.05) although there were differences from those of the control group. CONCLUSION: Low-energy semiconductor laser intranasal irradiation of the blood can effectively improve high blood coagulation status in healthy pregnant women at term.