RESUMO
Autophagy is a multi-step lysosomal degradation process, which regulates energy and material metabolism and has been used to maintain homeostasis. Autophagy has been shown to be involved in the regulation of health and disease. But at present, there is no consensus on the relationship between autophagy and tumour, and we consider that it plays a dual role in the occurrence and development of tumour. That is to say, under certain conditions, it can inhibit the occurrence of tumour, but it can also promote the process of tumour. Therefore, autophagy could be used as a target for tumour treatment. The regulation of autophagy plays a synergistic role in the radiotherapy, chemotherapy, phototherapy and immunotherapy of tumour, and nano drug delivery system provides a promising strategy for improving the efficacy of autophagy regulation. This review summarised the progress in the regulatory pathways and factors of autophagy as well as nanoformulations as carriers for the delivery of autophagy modulators.
Assuntos
Autofagia , Neoplasias , Autofagia/fisiologia , Homeostase , Humanos , Imunoterapia , Neoplasias/tratamento farmacológico , Neoplasias/metabolismoRESUMO
Molybdenum oxide (MoOx) nanosheets have drawn increasing attention for minimally invasive cancer treatments but still face great challenges, including complex modifications and the lack of efficient accumulation in tumor. In this work, a novel multifunctional degradable FA-BSA-PEG/MoOx nanosheet was fabricated (LA-PEG and FA-BSA dual modified MoOx): the synergistic effect of PEG and BSA endows the nanosheet with excellent stability and compatibility; the FA, a targeting ligand, facilitates the accumulation of nanosheets in the tumor. In addition, DTX, a model drug for breast cancer treatment, was loaded (76.49%, 1.5 times the carrier weight) in the nanosheets for in vitro and in vivo antitumor evaluation. The results revealed that the FA-BSA-PEG/MoOx@DTX nanosheets combined photothermal and chemotherapy could not only inhibit the primary tumor growth but also suppress the distant tumor growth (inhibition rate: 51.7%) and lung metastasis (inhibition rate: 93.6%), which is far more effective compared to the commercial Taxotere®. Exploration of the molecular mechanism showed that in vivo immune response induced an increase in positive immune responders, suppressed negative immune suppressors, and established an inflammatory tumor immune environment, which co-contributes towards effective suppression of tumor and lung metastasis. Our experiments demonstrated that this novel multifunctional nanosheet is a promising platform for combined chemo-photothermal therapy.
Assuntos
Materiais Biocompatíveis/química , Neoplasias da Mama/tratamento farmacológico , Neoplasias Pulmonares/tratamento farmacológico , Molibdênio/química , Nanoestruturas/uso terapêutico , Óxidos/química , Animais , Materiais Biocompatíveis/farmacocinética , Materiais Biocompatíveis/farmacologia , Materiais Biocompatíveis/uso terapêutico , Neoplasias da Mama/patologia , Linhagem Celular Tumoral , Sobrevivência Celular/efeitos dos fármacos , Doxorrubicina/química , Doxorrubicina/farmacologia , Doxorrubicina/uso terapêutico , Feminino , Ácido Fólico/química , Humanos , Hipertermia Induzida , Raios Infravermelhos , Neoplasias Pulmonares/secundário , Neoplasias Pulmonares/terapia , Camundongos , Camundongos Endogâmicos BALB C , Nanoestruturas/química , Nanoestruturas/toxicidade , Polietilenoglicóis/química , Soroalbumina Bovina/química , Ácido Tióctico/química , Distribuição TecidualRESUMO
The restricted tumor penetration has been regarded as the Achilles' Heels of most nanomedicines, largely limiting their efficacy. To address this challenge, a cluster-bomb-like nanoplatform named CPIM is prepared, which for the first time combines size-transforming and transcytosis strategies, thus enhancing both passive and active transport. For passive diffusion, the "cluster-bomb" CPIM (135 nm) releases drug-loaded "bomblets" (IR780/1-methyl-tryptophan (1 MT) loaded PAMAM, <10 nm) in response to the high reactive-oxygen-species (ROS) concentration in tumor microenvironment (TME), which promotes intratumoral diffusion. Besides, IR780 generates ROS upon NIR irradiation and intensifies this responsiveness; therefore, there exists a NIR-triggered self-destructive behavior, rendering CPIM spatiotemporal controllability. For active transport, the nanoplatform is proven to be delivered via transcytosis with/without NIR irradiation. Regarding the anti-cancer performance, CPIM strengthens the photodynamic therapy (PDT)/photothermal therapy (PTT) activity of IR780 and IDO pathway inhibition effect of 1 MT, thus exhibiting a strongest inhibitory effect on primary tumor. CPIM also optimally induces immunogenic cell death, reverses the "cold" TME to a "hot" one and evokes systemic immune response, thus exerting an abscopal and anti-metastasis effects. In conclusion, this work provides a facile, simple yet effective strategy to enhance the tumor penetration, tumor-killing effect and antitumor immunity of nanomedicines.
Assuntos
Nanopartículas , Fotoquimioterapia , Linhagem Celular Tumoral , Humanos , Fototerapia , Espécies Reativas de OxigênioRESUMO
Targeted drug delivery system for tumor cells is an appealing platform on enhancing the therapeutic effects and reducing the side effects of the drug. In this study, we developed folate-modified curcumin (Cur) loaded micelles (Cur-FPPs) for cancer chemotherapy. The targeting material, Folate-PEG3000-PLA2000, was synthesized by the amide bond formation reaction. And the Cur loaded micelles were prepared by thin-film hydration method with mPEG2000-PLA2000 (Cur-PPs) or mPEG2000-PLA2000 and Folate-PEG3000-PLA2000 (Cur-FPPs) as carrier. A central composite design (CCD) was used to optimize the formulation, and the optimized Cur-FPPs was prepared with the weight ratio of Folate-PEG3000-PLA2000 and mPEG2000-PLA2000 at 1:9. The average size of the mixed micelles was 70nm, the encapsulating efficiency and drug-loading were 80.73±0.16% and 4.84±0.01%, respectively. Compared with the Cur propylene glycol solution, the in vitro release of Cur from Cur-FPPs showed a sustained manner. Furthermore, the in vitro cytotoxicity and cellular uptake of Cur-FPPs were significantly enhanced towards MCF-7 and HepG2 cells. The pharmacokinetic studies in rats indicated that a 3-fold increase in the half-life was achieved for Cur loaded micelle formulations relative to solubilized Cur. All the results demonstrated that folate-modified Cur micelles could serve as a potential nanocarrier to improve the solubility and anti-cancer activity of Cur.
Assuntos
Curcumina/uso terapêutico , Sistemas de Liberação de Medicamentos , Ácido Fólico/química , Micelas , Neoplasias/tratamento farmacológico , Animais , Morte Celular/efeitos dos fármacos , Sobrevivência Celular/efeitos dos fármacos , Curcumina/administração & dosagem , Curcumina/farmacocinética , Curcumina/farmacologia , Endocitose/efeitos dos fármacos , Hemólise/efeitos dos fármacos , Células Hep G2 , Humanos , Irritantes/farmacologia , Ácido Láctico/síntese química , Ácido Láctico/química , Células MCF-7 , Masculino , Microscopia de Fluorescência , Tamanho da Partícula , Poliésteres , Polietilenoglicóis/síntese química , Polietilenoglicóis/química , Polímeros/síntese química , Polímeros/química , Coelhos , Ratos , Eletricidade Estática , Tensoativos/síntese química , Tensoativos/químicaRESUMO
Curcumin has been estimated as a potential agent for many diseases and attracted great attention owing to its various pharmacological activities, including anti-cancer, and anti-inflammatory. Now curcumin is being applied to a number of patients with breast cancer, rheumatoid arthritis, Alzheimer's disease, colorectal cancer, psoriatic, etc. Several clinical trials have stated that curcumin is safe enough and effective. The objective of this article was to summarize the clinical studies of curcumin, and give a reference for future studies.
Assuntos
Anti-Inflamatórios/uso terapêutico , Antineoplásicos Fitogênicos/uso terapêutico , Fármacos Cardiovasculares/uso terapêutico , Curcumina/uso terapêutico , Doença de Alzheimer/tratamento farmacológico , Anti-Inflamatórios/efeitos adversos , Anti-Inflamatórios/química , Antineoplásicos Fitogênicos/efeitos adversos , Antineoplásicos Fitogênicos/química , Fármacos Cardiovasculares/efeitos adversos , Fármacos Cardiovasculares/química , Ensaios Clínicos como Assunto , Curcumina/efeitos adversos , Curcumina/análogos & derivados , Curcumina/química , Vesícula Biliar/efeitos dos fármacos , Humanos , Resultado do TratamentoRESUMO
Currently, colon-specific drug delivery systems have been investigated for drugs that can exert their bioactivities in the colon. In this study, Eudragit® S100 coated calcium pectinate microsphere, a pH-dependent and enzyme-dependent system, as colon-specific delivery carrier for curcumin was investigated. Curcumin-loaded calcium pectinate microspheres were prepared by emulsification-linkage method, and the preparation technology was optimised by uniform experimental design. The morphology of microspheres was observed under scanning electron microscopy. Interactions between drug and polymers were investigated with differential scanning calorimetry (DSC) and X-ray diffraction. In vitro drug release studies were performed in simulated colonic fluid in the presence of Pectinex Ultra SP-L or 1% (w/v) rat caecal content, and the results indicated that the release of curcumin was significantly increased in the presence of 1% (w/v) rat caecal contents. It could be concluded that Eudragit® S100 coated calcium pectinate microsphere was a potential carrier for colon delivery of curcumin.
Assuntos
Colo/efeitos dos fármacos , Neoplasias do Colo/tratamento farmacológico , Curcumina/administração & dosagem , Sistemas de Liberação de Medicamentos/métodos , Pectinas/administração & dosagem , Ácidos Polimetacrílicos/administração & dosagem , Animais , Antineoplásicos/administração & dosagem , Antineoplásicos/química , Antineoplásicos/farmacologia , Varredura Diferencial de Calorimetria , Ceco/efeitos dos fármacos , Ceco/metabolismo , Materiais Revestidos Biocompatíveis/administração & dosagem , Materiais Revestidos Biocompatíveis/química , Materiais Revestidos Biocompatíveis/farmacologia , Colo/metabolismo , Neoplasias do Colo/metabolismo , Curcumina/química , Curcumina/farmacologia , Microscopia Eletrônica de Varredura , Microesferas , Pectinas/química , Pectinas/farmacologia , Ácidos Polimetacrílicos/química , Ácidos Polimetacrílicos/farmacologia , Ratos , Difração de Raios XRESUMO
The objective of this investigation was to develop solid lipid nanoparticles (SLNs) of penciclovir and evaluate the potential of SLNs as the carrier of penciclovir for topical delivery. Penciclovir-loaded SLNs were prepared by a double (W/O/W) emulsion technique. The SLNs presented spherical with the mean diameter of 254.9 nm. The entrapment efficiency, drug loading and zeta potential were 92.40%, 4.62% and -25.0 mV, respectively. DSC study showed that penciclovir encapsulated in SLNs was in the amorphous form. The cumulative amount of penciclovir penetrated through excised rat skin from SLNs was more than 2-fold that of the commercial cream as a control at 12h after administration. There was no significant difference of penciclovir content deposited in epidermis between the cream and SLNs administrated for 2, 6 and 12h, while SLNs increased the cumulative uptake of penciclovir in dermis significantly at the same intervals. Microscopic pictures showed that the interaction between SLNs and the skin surface changed the apparent morphology of stratum corneum and broke the close conjugation of corneocyte layers, which was the possible reason that SLNs increased the permeation of penciclovir into skin dermis. It can be concluded from our study that SLNs provide a good skin targeting effect and may be a promising carrier for topical delivery of penciclovir.
Assuntos
Aciclovir/análogos & derivados , Sistemas de Liberação de Medicamentos/métodos , Nanopartículas/administração & dosagem , Aciclovir/administração & dosagem , Aciclovir/química , Aciclovir/farmacocinética , Administração Tópica , Animais , Avaliação Pré-Clínica de Medicamentos/métodos , Guanina , Técnicas In Vitro , Masculino , Nanopartículas/química , Tamanho da Partícula , Ratos , Ratos Wistar , Absorção Cutânea/efeitos dos fármacos , Absorção Cutânea/fisiologiaRESUMO
Rhodococcus sp. PR-1 with high capability of demulsification on a surfactant-stabilized kerosene-water model emulsion was isolated from sewage of Dagang oil field. It could demulsify the model emulsion completely in 8 hours at 55 degrees C and had better demulsifying capability than chemical demulsifier DGF-01. The freezing-thawing and autoclaving had no effect on the process of demulsification, yet ultrasonic disposal and deal with organic solvent could inhibit its activity. A linear relationship, not the first order reaction that was used in some references, was observed between the percentage of demulsification and reaction time. The demulsifying capability of PR-1 was mainly resulted from the hydrophobic surfaces of microbial cells, which were characterized by the mycolic acids with the carbon-chain-length from 27 to 54. These results provide foundation for biological application on demulsifying crude oil emulsions in produced water of oil field.
Assuntos
Emulsificantes/metabolismo , Petróleo/metabolismo , Rhodococcus/isolamento & purificação , Rhodococcus/metabolismo , Biodegradação Ambiental , Emulsões , Petróleo/microbiologia , Esgotos/microbiologiaRESUMO
To prepare and optimize the gastrodin dispersible tablets by orthogonal design using the disintegration time as index. The quality of gastrodin dispersible tablets was evaluated by the initial stability test. The results showed that the disintegration time of optimized prescription formulation was 106s, i.e. L-HPC and CMS-Na was used by combining exterior and interior and the dissolution percent in vitro was obviously super to the conventional tablets. Moreover, the quality of the dispersible tablets was very well by stability test.
Assuntos
Álcoois Benzílicos/administração & dosagem , Medicamentos de Ervas Chinesas/administração & dosagem , Glucosídeos/administração & dosagem , Excipientes Farmacêuticos/administração & dosagem , Plantas Medicinais/química , Tecnologia Farmacêutica/métodos , Área Sob a Curva , Álcoois Benzílicos/química , Química Farmacêutica , Estabilidade de Medicamentos , Medicamentos de Ervas Chinesas/química , Glucosídeos/química , Lactose/administração & dosagem , Excipientes Farmacêuticos/química , Excipientes Farmacêuticos/classificação , Solubilidade , ComprimidosRESUMO
To estabilish a HPLC method for determination of quercetin-hydroxypropyl-beta-cyclodextrin in rats plasma and investigate the pharmacokinetics characteristics of it in rats. The plasma was extracted with methanol-aceitc acid (9:1). The mobile phase was acetonitrilewater. Absorbance of the effluence was monitored at 360 nm. The linear limit of quercetin was within the range of 1 to 150 microg/ml. The lowest limit of quercetin was 0.5 microg/ml. The concentration-time curve of quercetin in rats was considered of a two-compartment open model, and the t1/2 (beta) of the inclusion compound when ig is 2.220 h, the t1/2 (beta) of the inclusion compound when iv is 90.871 min.