[The protective effects and mechanisms of Trillium tschonoskii Maxim on rats' liver damage induced by lipopolysaccharide].

OBJECTIVE: To study the protective effects of Trillium tschonoskii maxim (TTM) on rats' oxidative stress and hepatotoxicity in-duced by lipopolysaccharide (LPS). METHODS: Sixty SD rats were randomly divided into TTM high, medium and low dose groups, model group, Dexamethasone (DEX) control group and blank control group with ten rats in each group. The TTM high, medium and low dose groups were treated with 8, 4, 2 g/(kg·d) TTM by intragastric administration and model group, DEX control group and blank control group were treated with the same amount of distilled water respectively. The TTM high, medium and low dose groups, model group, DEX control group were injected intraperitoneal with 1 mg/kg LPS and the DEX control group was injected intraperitoneal with 5 mg/kg DEX, the blank control group was injected with same amount of normal saline every five days. The indexes of rats' thymus and spleen were measured in 30 days. The activities of serum nitric oxide synthase (NOS), superoxide dismutase (SOD) and the contents of nitric oxide (NO), glutathione (GSH), glu-cosinolates barbituric acid reaction product(TBARS), white cells interleukin-6(IL-6), IL-10 and tumor necrosis factor-α(TNF-α), the activi-ties of liver SOD, GSH-Px and the contents of GSH and TBARS were measured. RESULTS: TTM high dose group was significantly different in body weight in 19~30 days(P<0.05); The index of thymus in TTM high, medium and low dose groups and the index of spleen in TTM high dose group were decreased significantly compared with those of the model group. The activity of serum NOS and the contents of TBARS and NO in TTM high, medium and low dose groups were decreased significantly(P<0.05). The activity of serum SOD in TTM high dose group and the contents of GSH in TTM medium and low dose groups were increased significantly(P<0.05). The contents of serum IL-6 and TNF-αin TTM high, medium dose groups were decreased significantly and the contents of serum IL-10 were increased significantly(P<0.05). The con-tents of liver TBARS in TTM high, medium dose groups were decreased significantly. The activity of liver SOD in TTM high, medium and low dose groups, the activity of GSH-Px in TTM high, medium dose groups and the contents of GSH in TTM high dose group were increased signifi-cantly(P<0.05). CONCLUSIONS: TTM has a certain effect to delay the rats' atrophy of thymus and spleen generated by LPS. It can effectively reduce the activity of NOS in serum, reduce the formation of NO, improve the activity of SOD, GSH-Px and the contents of GSH, reduce lipid peroxidation, decrease the excessive secretion of IL-6, TNF-α, increase the contents of IL-10, which can resist inflammation and protect the liver.

[The effect of Wu-He Dipsacus asper on mice-aging model induced by D-galactose].

OBJECTIVE: To study the effect of Wu-He Dipsacus asper (WHDA), Traditional Chinese Medicine, injection on mice-aging model induced by D-galactose. METHODS: Forty-eight Kunming mice (24 male and 24 female) were randomly divided into control group, model group, positive control group, 7.2 g/kg WHDA group, 3.6 g/kg WHDA group and 1.8 g/kg WHDA group with eight in each group. The model was induced through injecting D-galactose into peritoneal cavity and Morris water maze was used to detect the learning and cognitive ability of mice. The skin hydroxyproline, brain tissue malondialdehyde (MDA), lipofuscin (LP), superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) levels of mice were detected; the IL-2 and IL-6 levels in serum of mice were detected by using double antibody sandwich ELISA method. RESULTS: Each WHDA group was significantly reduced in latency period compared with the model group during Morris water maze test (P < 0.05) and the number of mice in model group through the platform was less than other mice in each group (P < 0.05). The levels of MAD and LP of the control group and each WHDA group were less than model group in the detection of heart, brain tissue oxidation index (SOD, MAD, LP and GSH-Px, P < 0.05). The activity of SOD and GSH-Px in the control group and each WHDA group was significantly higher than that in the model group (P < 0.05). The skin hydroxyproline content of mice which had been injected with D-galactose was significantly lower than that in the control group (P < 0.05) and the skin hydroxyproline content of mice of WHDA group was significantly higher than that in the model group (P < 0.05). The IL-2, IL-6 levels in serum of mice in WHDA group were significantly higher than those in the control group and the model group (P < 0.05) and the IL-2, IL-6 levels in serum of mice in the model group were lower than those in the control group (P < 0.05). CONCLUSION: The effective constituents of WHDA have a variety of biological activity which can have a good effect on anti-aging by different ways, improving learning and memory function, eliminating free radicals antioxidant, and enhancing the body immunity and other aspects.