RESUMO
Isolariciresinol-9'-O-α-L-arabinofuranoside (MWS19) isolated from Pinus massoniana Lamb. Fresh pine needles is the major ingredient of the Songling Xuemaikang capsule therapy used for hypertension. The present study aimed to investigate the effects and underlying mechanisms of MWS19 on hydrogen peroxide (H2O2)induced apoptosis in human umbilical vein endothelial cells (HUVECs). To investigate the effect of MWS19 on apoptosis in HUVECs, an oxidative stressinduced apoptosis model was established in HUVECs using H2O2, and the present study performed Hoechst 33258 staining and a Cell Counting Kit8 (CCK8) assay. Furthermore, western blot analysis was also performed to investigate the underlying mechanism of the effects of MWS19 on the model. The results demonstrated that MWS19 reversed the effects of H2O2 on cell apoptosis at a concentration range of 15.6250 µg/ml, with dosedependent increases in cell growth. Hoechst staining indicated that 500 µM H2O2 induced HUVEC apoptosis, and MWS19 markedly protected HUVECs against apoptosis at 31.3, 62.5 and 125 µg/ml. Furthermore, the protein expression of phosphatidylinositol 3kinase (PI3K), phosphorylatedAkt and Bcl2associated agonist of cell death (Bad) were increased, and reduced caspase3 activation was observed, following treatment with MWS19 in H2O2treated HUVECs. Additionally, the PI3K inhibitor wortmannin attenuated PI3K/Akt/Bad signaling induced by MWS19 treatment and neutralized the effect of MWS19 on the growth of HUVECs. In conclusion, the results of the present study indicate that MWS19 may protect against H2O2induced HUVEC apoptosis via the PI3K/Akt/Bad signaling pathway. MWS19 may serve an important role in the prevention of oxidative damage in vascular endothelial cells in hypertension patients.
Assuntos
Apoptose/efeitos dos fármacos , Peróxido de Hidrogênio/farmacologia , Fosfatidilinositol 3-Quinase/metabolismo , Extratos Vegetais/farmacologia , Proteínas Proto-Oncogênicas c-akt/metabolismo , Transdução de Sinais/efeitos dos fármacos , Proteína de Morte Celular Associada a bcl/metabolismo , Sobrevivência Celular/efeitos dos fármacos , Células Endoteliais da Veia Umbilical Humana , HumanosRESUMO
Two new sesquiterpene glycosides, namely massonside A (1) and massonside B (2), were isolated from the n-Bu extract of the fresh needles of Pinus massoniana Lamb. Their structures were established by 1D, 2D nuclear magnetic resonance and high-resolution mass spectrometry. Their biological activities were profiled by the anti-HBV and anti-HCV assays.