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Tohoku J Exp Med ; 222(3): 219-23, 2010 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-21079380

RESUMO

Re-stenosis or remodeling of coronary and peripheral arteries remains a major complication following balloon-angioplasty or stenting. This study was designed to investigate the effect of nesiritide, a recombinant B-type natriuretic peptide (BNP), on vascular remodeling following balloon-induced endothelial injuries. Twenty-eight male New Zealand rabbits were divided into nesiritide-treated (0.1 mg/kg/day, sc, for 4 weeks, n = 10), saline-treated control (n = 10) and sham-operated groups (n = 8). In the nesiritide and control groups, a balloon catheter was inserted to the right iliac artery to induce injuries. Vascular endothelial growth factor (VEGF) was measured by immunohistochemistry. The area under internal elastic membrane of the arterial wall (643.2 ± 134.1 vs 493.7 ± 139.3 µm(2), p < 0.05) and the area under external elastic membrane (1495.1 ± 204.9 vs 1265.9 ± 232.6 µm(2), p < 0.05) in the nesiritide group were greater than those in the control group, but were smaller than those in the sham-operated group (p < 0.05). The stenosis ratio was lower in the nesiritide group than in the control group (18.7 ± 7.7% vs 38.0 ± 8.3%, p < 0.01). Importantly, the VEGF expression rate was significantly lower in the nesiritide group than in the control group (42.2 ± 8.8% vs 56.1 ± 13.1%, p < 0.05), while there were no signs of VEGF expression in the non-injured arteries of the three groups. In conclusion, nesiritide treatment reduces the stenosis of the rabbit iliac artery following balloon-induced endothelial injuries probably by decreasing VEGF expression.


Assuntos
Angioplastia com Balão/efeitos adversos , Vasos Sanguíneos/efeitos dos fármacos , Endotélio Vascular/efeitos dos fármacos , Peptídeo Natriurético Encefálico/uso terapêutico , Doenças Vasculares/tratamento farmacológico , Doenças Vasculares/etiologia , Animais , Vasos Sanguíneos/lesões , Vasos Sanguíneos/patologia , Vasos Sanguíneos/fisiologia , Constrição Patológica/tratamento farmacológico , Constrição Patológica/etiologia , Constrição Patológica/metabolismo , Constrição Patológica/patologia , Avaliação Pré-Clínica de Medicamentos , Endotélio Vascular/metabolismo , Endotélio Vascular/patologia , Artéria Ilíaca/efeitos dos fármacos , Artéria Ilíaca/lesões , Artéria Ilíaca/metabolismo , Artéria Ilíaca/patologia , Masculino , Natriuréticos/farmacologia , Natriuréticos/uso terapêutico , Peptídeo Natriurético Encefálico/análogos & derivados , Peptídeo Natriurético Encefálico/farmacologia , Coelhos , Proteínas Recombinantes/farmacologia , Proteínas Recombinantes/uso terapêutico , Doenças Vasculares/metabolismo , Doenças Vasculares/patologia , Fator A de Crescimento do Endotélio Vascular/metabolismo
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