RESUMO
Nutrients releasing from anoxic sediment can be enhanced in summer because the dissolved oxygen (DO) consumption, nitrogen (N) and phosphorus (P) migration are susceptible to temperature. Herein, we proposed a method to hinder the aquatic environmental deterioration in warm seasons through consecutive application of oxygen- and lanthanum-modified zeolite (LOZ) and submerged macrophytes (V. natans) at low temperature scenario (5 °C, with depleted DO in water), and the effect was examined with drastic increasing the ambient temperature to 30 °C. The investigation was conducted in a microcosm scale including sediment cores (with a diameter of 11 cm, height of 10 cm) and overlying water (with depth of 35 cm). During the 60 days experiment, application of LOZ at 5 °C facilitated slower releasing and diffusion of oxygen from LOZ and the growth of V. natans. Thereby, when the temperature was increased to 30 °C and maintained for 35 days, the DO reached 10.01 mg/L, and the release of P and N from the sediment was reduced by 86% and 92%, respectively. This was achieved from the joint efforts of adsorption, biological conversion, chemical inactivation, and assimilation. Also, the LOZ inhibited 80% N2O, 75% CH4, and 70% CO2 emissions primary by promoting V. natans growth and reshaping microbiota. Meanwhile, the colonization of V. natans benefited the sustainable improvement in the water quality. Our results addressed the time that the remediation of anoxic sediment can be applied.
Assuntos
Zeolitas , Oxigênio , Temperatura , Lagos , Qualidade da Água , Fósforo/análise , Nitrogênio/análise , Sedimentos GeológicosRESUMO
Lung cancer is the leading cause of cancer-related death worldwide. Here we investigated the antitumor effect and mechanism of Zhejiang (Huzhou and Jiande) saffron against lung cancer cell lines, A549 and H446. Using high performance liquid chromatography (HPLC), the contents of crocin I and II were determined. In vitro, MTT assay and annexin-V FITC/PI staining showed cell proliferation activity and apoptosis to be changed in a dose- and time-dependent manner. The inhibition effect of Jiande saffron was the strongest. In vivo, when mice were orally administered saffron extracts at dose of 100mg/kg/d for 28 days, xenograft tumor size was reduced, and ELISA and Western blotting analysis of caspase-3, -8 and -9 exhibited stronger expression and activity than in the control. In summary, saffron from Zhejiang has significant antitumor effects in vitro and in vivo through caspase-8-caspase-9-caspase-3 mediated cell apoptosis. It thus appears to have more potential as a therapeutic agent.