Determination of geographic origins and types of Lindera aggregata samples using a portable short-wave infrared hyperspectral imager.

A portable short-wavelength infrared microscope hyperspectral imager (SMHI) combined with machine learning algorithms for the purpose of classifying geographical origins as well as root types of Lindera aggregata is developed. The spectral range of the SMHI system is 1090-1820 nm (5500-9100 cm-1) with spectral and spatial resolutions of 4 nm and 27.3 µm, respectively. Utilizing PCA-RF algorithms, the geographic origin of tuberous roots and leaves from five different origins were classified with accuracies of 97.5% and 97.8%, respectively. In addition, spatial identification of tuberous root and taproot tubers in a mixed sample was done with an accuracy of 98.98%. The accuracy of origin classification and spatial identification are high enough which indicate the significant potential of applying SMHI system into the non-invasive spatial mapping and rapid quality assessment of medicinal herbs.

Confocal hyperspectral microscopic imager for the detection and classification of individual microalgae.

We propose a confocal hyperspectral microscopic imager (CHMI) that can measure both transmission and fluorescent spectra of individual microalgae, as well as obtain classical transmission images and corresponding fluorescent hyperspectral images with a high signal-to-noise ratio. Thus, the system can realize precise identification, classification, and location of microalgae in a free or symbiosis state. The CHMI works in a staring state, with two imaging modes, a confocal fluorescence hyperspectral imaging (CFHI) mode and a transmission hyperspectral imaging (THI) mode. The imaging modes share the main light path, and thus obtained fluorescence and transmission hyperspectral images have point-to-point correspondence. In the CFHI mode, a confocal technology to eliminate image blurring caused by interference of axial points is included. The CHMI has excellent performance with spectral and spatial resolutions of 3 nm and 2 µm, respectively (using a 10× microscope objective magnification). To demonstrate the capacity and versatility of the CHMI, we report on demonstration experiments on four species of microalgae in free form as well as three species of jellyfish with symbiotic microalgae. In the microalgae species classification experiments, transmission and fluorescence spectra collected by the CHMI were preprocessed using principal component analysis (PCA), and a support vector machine (SVM) model or deep learning was then used for classification. The accuracy of the SVM model and deep learning method to distinguish one species of individual microalgae from another was found to be 96.25% and 98.34%, respectively. Also, the ability of the CHMI to analyze the concentration, species, and distribution differences of symbiotic microalgae in symbionts is furthermore demonstrated.

Machine learning classification of origins and varieties of Tetrastigma hemsleyanum using a dual-mode microscopic hyperspectral imager.

A dual-mode microscopic hyperspectral imager (DMHI) combined with a machine learning algorithm for the purpose of classifying origins and varieties of Tetrastigma hemsleyanum (T. hemsleyanum) was developed. By switching the illumination source, the DMHI can operate in reflection imaging and fluorescence detection modes. The DMHI system has excellent performance with spatial and spectral resolutions of 27.8 µm and 3 nm, respectively. To verify the capability of the DMHI system, a series of classification experiments of T. hemsleyanum were conducted. Captured hyperspectral datasets were analyzed using principal component analysis (PCA) for dimensional reduction, and a support vector machine (SVM) model was used for classification. In reflection microscopic hyperspectral imaging (RMHI) mode, the classification accuracies of T. hemsleyanum origins and varieties were 96.3% and 97.3%, respectively, while in fluorescence microscopic hyperspectral imaging (FMHI) mode, the classification accuracies were 97.3% and 100%, respectively. Combining datasets in dual mode, excellent predictions of origin and variety were realized by the trained model, both with a 97.5% accuracy on a newly measured test set. The results show that the DMHI system is capable of T. hemsleyanum origin and variety classification, and has the potential for non-invasive detection and rapid quality assessment of various kinds of medicinal herbs.