Effect of stored autologous blood transfusion on function of bone marrow hematopoietic stem cells and recovery of reticulocytes in peripheral blood of rabbits / 中华麻醉学杂志

Objective:To evaluate the effect of stored autologous blood transfusion on the function of bone marrow hematopoietic stem cells and the recovery of reticulocytes in peripheral blood of rabbits.Methods:Thirty healthy male New Zealand rabbits, aged 3-6 months, weighing 2.5-3.0 kg, were divided into 5 groups ( n=6 each) using a random number table method: control group (C group), operation group (O group), blood collection group (O+ B group), stored autologous whole blood group (O+ WB group) and stored autologous component blood group (O+ CB group). In O+ B group, O+ WB group and O+ CB group, blood was collected via the right femoral artery at a rate of 3-5 ml/min at 7 days before surgery, the blood volume was 10% of the total blood volume, and blood was stored at 4-6 ℃.In O+ CB group, blood was centrifuged to remove plasma and white blood cells, and red blood cell suspension was prepared and placed in a blood storage bag.In O, O+ B, O+ WB and O+ CB groups, hepatectomy was performed, and bleeding was performed through the right femoral artery at a rate of 3-5 ml/min, and the blood volume was 10% of the total blood volume.Lactated Ringer′s solution 1 ml/min was intravenously infused during surgery in O and O+ B groups, stored autologous whole blood was intravenously infused during surgery in group O+ WB, and stored autologous red blood cell suspension 1 ml/min was intravenously infused during surgery in group O+ CB.Bone marrow blood samples were collected immediately before the start of surgery (T 2) and at 6 and 24 h after surgery (T 3, 4) to determine the percentage of CD34 + cells, cell cycle and relative expression of telomere DNA.Peripheral venous blood samples were collected before storage (T 1) and at T 4 for determination of the percentage of reticulocytes. Results:Compared with group C, the percentage of CD34 + cells was significantly increased at T 3 and T 4, the percentage of CD34 + cells in G1 phase was decreased, and the expression of telomere DNA in CD34 + cells was up-regulated in group O, and the percentage of reticulocyte in peripheral blood was increased at T 4 in the remaining 4 groups ( P<0.05). Compared with group O, the percentage of CD34 + cells was significantly increased at T 2-4, the percentage of CD34 + cells in G1 phase was decreased, and the expression of telomere DNA in CD34 + cells was up-regulated in O+ B, O+ WB and O+ CB groups, and the percentage of reticulocyte in peripheral blood was increased at T 4 in O+ WB and O+ CB groups ( P<0.05). Compared with group O+ B, the percentage of CD34 + cells was significantly increased at T 3 and T 4, the percentage of CD34 + cells in G1 phase was decreased, and the expression of telomere DNA in CD34 + cells was up-regulated in O+ WB and O+ CB groups, and the percentage of reticulocyte in peripheral blood was decreased at T 4 in O+ WB and O+ CB groups ( P<0.05). Compared with O+ WB group, the percentage of CD34 + cells was significantly decreased at T 3 and T 4, the percentage of CD34 + cells in G 1 phase was increased, the expression of telomere DNA in CD34 + cells was down-regulated, and the percentage of reticulocytes in peripheral blood was increased at T 4 in group O+ CB ( P<0.05). Conclusion:Stored autologous blood transfusion can inhibit the function of bone marrow hematopoietic stem cells and is not helpful for recovery of reticulocytes in peripheral blood of rabbits; stored autologous component blood transfusion has less effect on the hematopoietic function of bone marrow than stored autologous whole blood transfusion.

Correlation Between RhoA Gene in Wnt/PCP Signaling Pathway and Traditional Chinese Medicine Syndrome of AECOPD / 中国实验方剂学杂志

Objective: To investigate the correlation between Ras homolog gene family member A (RhoA) gene in Wnt/PCP signaling pathway and acute exacerbation chronic obstructive pulmonary disease (AECOPD) traditional Chinese medicine(TCM)syndrome, attempting to provide an objective standard for the diagnosis of AECOPD TCM syndrome. Method: The 100 AECOPD patients were collected and divided into 5 groups:phlegm turbid obstructing lung syndrome,, phlegm-heat obstructing lung syndrome, syndrome of orifices confused by phlegm, deficiency of pulmonary and renal Qi, and edema due to yang deficiency, with 20 people in each group. 15 normal people were selected as a normal control group. All patients received fasting hemospasia, using a kit to extract blood total RibonucleicA(RNA) according to instructions. Real-time quantitative polymerase chain reaction (Real-time PCR) was adopted to detect the mRNA expression of RhoA gene in blood of patients with AECOPD TCM syndrome, and to explore the correlation. Result: There was no difference between phlegm-heat obstructing lung syndrome group and syndrome of orifices confused by phlegm group. The mRNA expression of RhoA gene in phlegm turbid obstructing lung syndrome group, phlegm-heat obstructing lung syndrome group, syndrome of orifices confused by phlegm group, deficiency of pulmonary and renal Qi group, and edema due to Yang deficiency group were significantly higher than that in normal group (PConclusion: The significant difference in mRNA relative expression of RhoA gene in Wnt/PCP signaling pathway among the five AECOPD TCM syndrome groups may provide some objective diagnostic criteria for AECOPD TCM syndromes and reveal their disease severity.

Senile habitual constipation treated with auricular therapy based on the pattern/syndrome differentiation: a randomized controlled trial / 中国针灸

<p><b>OBJECTIVE</b>To compare the efficacy on senile habitual constipation between the auricular therapy based on the pattern/syndrome differentiation and the conventional auricular point sticking therapy.</p><p><b>METHODS</b>Two-hundred cases were randomized into a group of the auricular therapy based on the pattern/syndrome differentiation (an auricular differentiation group) and a group of conventional auricular point sticking therapy (an auricular sticking group), 100 cases in each one. In the auricular differentiation group, Brain Stem (AT3,4i), Occiput (AT3), Subcortex (AT4), Large Intestine (CO7), Triple Energizer (CO17), Abdomen (AH8), Endocrine (CO18) and Constipation Point were taken as the main points. According to the pattern/syndrome differentiation, for excessive heat in the stomach and intestine, Stomach (CO4) and Small Intestine (CO6) were added; for blockage of lung qi, Lung (CO14) was added; for spleen and kidney deficiency, Spleen (CO13) and Kidney (CO10) were added. In the auricular sticking group, CO7, Rectum (HX2), Sympathetic Nerve (AH6a) and AT4 were selected. In both groups, the patients were required to press the points four times a day by themselves. The auricular points were changed once every 3 to 4 days, and the two ears were alternated. The eight treatments made one session and two sessions were required totally. The clinical efficacy and the changes of the score for the quality of life before and after treatment were observed in the two groups.</p><p><b>RESULTS</b>The effective rate was 92.0% (92/100) in the auricular differentiation group, which was superior to 76.0% (76/100) in the auricular sticking group (P < 0.05). The score for the quality of life after treatment was reduced to different extents for the patients in the two groups (both P < 0.05). The score decrease in the auricular differentiation group was much more apparent as compared with that in the auricular sticking group (P < 0.05).</p><p><b>CONCLUSION</b>The auricular therapy based on the pattern/syndrome differentiation is safe and effective in the treatment of senile habitual constipation and its efficacy is superior to the conventional auricular point sticking therapy.</p>

Lymph node image with ultrasmall superparamagnetic iron oxide and comparison with pathological result / 中国医学科学院学报

<p><b>OBJECTIVE</b>To assess the characteristics of enhanced magnetic resonance image with ultrasmall superparamagnetic iron oxide (USPIO) in the inflammatory and tumor metastatic rabbit model, and explore its relevance with histologic ultrastructural findings.</p><p><b>METHODS</b>Totally 36 New Zealand white rabbits were randomly divided into lymphadenitis group and metastatic group. Complete Freund's adjuvant was injected into the bilateral dorsal footpads of 18 rabbits to set up ipsilateral lymphadenitis model. The other 18 rabbits received a subcutaneous implantation of VX2 tumor cell suspension (1.5 x 10(7) cells/ml) in both thighs to set up metastatic lymph node model. Magnetic resonance scan were performed 24 hours before and after USPIO (90 micromol Fe/kg) injection. T2 values of each lymph node were measured and lymph node T2 enhancement rate was calculated as well. HE staining, Prussian blue staining, and electronic microscopy were performed to observe the pathological microstructure changes and the distribution of the iron particle in lymph node. Relationship between lymph nodes USPIO enhancement and its microstructures were further analyzed. Results Thirty-six lymph nodes in lymphadenitis group showed different degrees of reactive hyperplasia. Twenty-six lymph nodes in metastatic group were invaded by tumor cell. Non-enhanced scan showed mild difference between T2 signal intensity of the two pathological lymph node types. After USPIO enhancement, inflammatory lymph nodes showed distinct T2 signal reduction at the center, and metastatic lymph nodes showed homogenous and faint T2 signal reduction. Enhancement rate of benign and malignant lymph nodes were 57.39% and 29.45% respectively (P < 0.01). HE staining and Prussian blue staining indicated USPIO particles located mainly in the macrophages at inflammatory lymphatic medulla, while paracortical area and cortical area contained relatively much less USPIO particles due to less macrophages distribution. MRI findings were correlated with the pathological results. Electronic microscopy also verified that the majority of USPIO particles were located in the numerous cytophagic bubbles of macrophages. Lymph nodes metastasis including 4 lymph nodes with completed structure destruction due to entire tumor infiltration, 19 lymph nodes with partially lymph node structure destruction but reduced USPIO-contained macrophage numbers or reduced USPIO particles in macrophages, and 3 lymph nodes with only localized foci tumor metastasis at subcapsular area. Conclusions USPIO enhancement pattern of different lymph nodes is closely related to distribution and functional status of the intra-node macrophages. It may affect the accuracy of the lymph node property diagnosis based on USPIO enhanced image.</p>

Studies on antifungal activity of 23 kinds of traditional Chinese medicines in extro-experiments / 重庆医科大学学报

Objective:To research the in vitro antifungal activity of 23 kinds of TCM against microsponrrn gypseum and trichophyton ncbrurn, the main causes of superficial dermatophyte. Methods:Semisolid agar dilution method was applied. Results:Some kinds of TCM showed strong antifungal activity against one or several kinds of fungi. Conclusion:Belamcandae, cuscuta seed,coptis chinensis,phellodendri and scutellaria are active in fungistasis in the extro-experiment.

Studies on antibacterial activity of different preparations of 10 kinds of Chinese traditional medicine in vitro-experiment / 中华中医药杂志

Objective:To observe the antibacterial activity of different preparations of 10 kinds of Chinese traditional medicine to Taphylococcus aureus,Staphylococcus epidermidis,Bacillus coli,Bacillus typhosus and Bacillus aeruginosus in vitro-experiment. Methods:Liquid agar dilution method was used. Results:The antibacterial activity of coptis chinensis is the best,scutellaria is the second. The antibacterial activity of tincture is better than that of decoction. Conclusions:The antibacterial activity of coptis chinensis is best during the 10 kinds of Chinese traditional medicine,and that of tincture is the best during different preparations.