RESUMO
OBJECTIVE: To optimize the conditions of extraction and purification of isocorydine from Dicranostigma leptopodum. METHODS: Extraction conditions of isocorydine were selected on the basis of orthogonal experimental design, the static adsorption/desorption experiments were used to evaluate the optimum resin. RESULTS: The optimum extraction parameters were as follows: the ratio of raw materials to solvent 1:15 (g/mL), extraction solvent 1% vitriol, the extraction three times and 1 h each time. LX28 resin exhibited higher adsorption efficiency. CONCLUSION: Under the above optimum conditions, the extraction yield of isocorydine is 0.88%. The purity of isocorydine can reach 85.34% with a yield rate of 68.64%.
Assuntos
Aporfinas/isolamento & purificação , Papaveraceae/química , Resinas Sintéticas/química , Tecnologia Farmacêutica/métodos , Adsorção , Aporfinas/química , Cromatografia Líquida de Alta Pressão , Medicamentos de Ervas Chinesas/química , Medicamentos de Ervas Chinesas/isolamento & purificação , Metanol/química , Porosidade , UltrassomRESUMO
OBJECTIVE: To study the chemical constituents from Clematis brevicaudata. METHODS: The compounds were isolated by column chromatography and their structures were elucidated through spectroscopic analysis (NMR). RESULTS: Eight compounds were isolated and identified as: palmitic acid (1), 1-docosanol (2), pentacosanoic acid-2', 3'-dihydroxypropyl ester (3), beta-sitosterol (4), daucosterol (5), a mixture of the trans-p-coumarate of the n-alkanols (6), 3,4-dihydroxy-trans coumatate ethyl ester (7), syringaresinol-O-D-glucopyranoside (8). CONCLUSION: All these compounds are obtained from Clematis brevicaudata for the first time.
Assuntos
Clematis/química , Medicamentos de Ervas Chinesas/química , Ácido Palmítico/química , Plantas Medicinais/química , Sitosteroides/química , Medicamentos de Ervas Chinesas/isolamento & purificação , Glucosídeos/química , Glucosídeos/isolamento & purificação , Lignanas/química , Lignanas/isolamento & purificação , Espectroscopia de Ressonância Magnética , Ácido Palmítico/isolamento & purificação , Sitosteroides/isolamento & purificaçãoRESUMO
OBJECTIVE: To isolate and identify the chemical constituents from the whole plant of Pyrethrum tatsienense, which has been used as traditonal herb medicine in the treatment of some diseases. METHODS: The compounds were isolated by column chromatography and their structures were elucidated through spectroscopic analysis (NMR). RESULTS: Six compounds were isolated and identified as: linoeic acid, pinoresinol, (+)-pinoresinol-4'-O-beta-D- glucopyranoside, betulabuside A, dihydrosyringin. CONCLUSION: All these compounds were obtained from Pyrethrum tatsienense for the first time.
Assuntos
Chrysanthemum cinerariifolium/química , Furanos/isolamento & purificação , Lignanas/isolamento & purificação , Ácidos Linoleicos/isolamento & purificação , Plantas Medicinais/química , Medicamentos de Ervas Chinesas/química , Medicamentos de Ervas Chinesas/isolamento & purificação , Furanos/química , Glucosídeos/química , Glucosídeos/isolamento & purificação , Lignanas/química , Ácidos Linoleicos/química , Espectroscopia de Ressonância Magnética/métodos , Estrutura Molecular , Monoterpenos/química , Monoterpenos/isolamento & purificaçãoRESUMO
Two novel and eight known triterpenoids were isolated from alcoholic extract of the whole plant of Pyrethrum tatsienense. The structures of new compounds were elucidated as olean-12-en-3beta,11alpha,16beta-triol-3-O-palmitate (1), ursa-12-en-3beta,11beta,16beta-triol-3-O-palmitate (2) by spectroscopic methods including intensive 2D NMR techniques (H1-H1 COSY, gHMQC, gHMBC) and FAB-MS, and the known compounds were identified on the basis of comparing their NMR data with those of corresponding compounds in the literature.
Assuntos
Chrysanthemum cinerariifolium/química , Triterpenos/química , Etanol , Espectroscopia de Ressonância Magnética , Extratos Vegetais/química , Solventes , Espectrometria de Massas de Bombardeamento Rápido de Átomos , Triterpenos/isolamento & purificaçãoRESUMO
OBJECTIVE: To study the alkaloid constituents of Corydalis adunca. METHOD: The constituents were isolated on silica gel column and their structures were elucidated by IR, NMR, MS data. RESULT: Eight alkaloid compounds were isolated from alcohol extracts of the herb of C. adunca, and identified as dihydrosanguinarine (I), tetrahydrocolumbamine (II), 1,2,3,4-tetrahydro-7-methoxy-1-[(4-methoxy)phenyl]methyl-8-quinolinol (III), protopine (IV) and 6-acetonyl-5,6-dihydrosanguinarine (V). CONCLUSION: Five compounds were isolated from C. adunca for the first time.