Shining light on chiral inorganic nanomaterials for biological issues.

The rapid development of chiral inorganic nanostructures has greatly expanded from intrinsically chiral nanoparticles to more sophisticated assemblies made by organics, metals, semiconductors, and their hybrids. Among them, lots of studies concerning on hybrid complex of chiral molecules with achiral nanoparticles (NPs) and superstructures with chiral configurations were accordingly conducted due to the great advances such as highly enhanced biocompatibility with low cytotoxicity and enhanced penetration and retention capability, programmable surface functionality with engineerable building blocks, and more importantly tunable chirality in a controlled manner, leading to revolutionary designs of new biomaterials for synergistic cancer therapy, control of enantiomeric enzymatic reactions, integration of metabolism and pathology via bio-to nano or structural chirality. Herein, in this review our objective is to emphasize current research state and clinical applications of chiral nanomaterials in biological systems with special attentions to chiral metal- or semiconductor-based nanostructures in terms of the basic synthesis, related circular dichroism effects at optical frequencies, mechanisms of induced optical chirality and their performances in biomedical applications such as phototherapy, bio-imaging, neurodegenerative diseases, gene editing, cellular activity and sensing of biomarkers so as to provide insights into this fascinating field for peer researchers.

Influence of leaf age, species and soil depth on the authenticity and geographical origin assignment of green tea.

RATIONALE: Stable isotope fractionation occurring during leaf growth provides internal characteristics for identifying the geographical origin, traceability and authentication of tea. Studying the influence of leaf age, species and the relationship with the cultivated soil may reveal previously undocumented stable isotope fractionation mechanisms, and provide a deeper understanding of the physiological isotopic effects on the tractability and authentication accuracy of green tea to combat mislabeling and fraudulent conduct. METHODS: A total of 36 pairs of young (one bud with one leaf) and mature growth (older leaf) samples from two species of Longjing tea (Longjing #43 and Colonial cultivar) and corresponding cultivation soil samples from two different depth layers (0-20 cm and 20-40 cm) were collected in Westlake district, Hangzhou, Zhejiang province, China. Four stable isotope ratios (δ13 C, δ15 N, δ2 H, and δ18 O values) were measured using an elemental analyzer coupled with an isotope ratio spectrometer. Linear correlation and one-way analysis of variance (ANOVA) statistical analyses were performed to investigate isotopic fractionation mechanisms during plant growth, and reflect the dynamic physiological processes from soil to leaf. RESULTS: The carbon and nitrogen isotope ratios (δ13 C and δ15 N values) reflected the absorption, migration and fractionation of carbon dioxide and nitrogenous nutrients during photosynthesis, nutrient uptake, nitrogen fixation and leaf respiration. The water isotope ratios (δ2 H and δ18 O values) reflected the use and fractionation of water by tea plants at different growth stages. CONCLUSIONS: Considerable differences were found for hydrogen and oxygen isotope ratios according to leaf age, revealing complex isotopic fractionation mechanisms and possible interference factors. Leaf maturity effects should be considered, as they will influence the precision and accuracy of models when assigning the geographical origin, traceability and authentication of tea.