Antiparasitic efficacy of flavonoids identified from Psoralea corylifolia against Tetrahymena piriformis in guppy (Poecilia reticulate).

Tetrahymena piriformis belongs to the ciliated protists (ciliates), causing severe economic losses in aquaculture. Chemical drugs currently used usually have toxic side effects, and there is no specific drug against Tetrahymena. Therefore, it is an urgent need to identify new antiparasitic lead compounds. In the present study, the in vitro parasiticidal activity of ethyl acetate (EtOAc) extracts and water extracts from 22 selected traditional Chinese medicines (TCMs) were evaluated against T. piriformis. The EtOAc extract of P. corylifolia turned out to be the most active with the minimum parasiticidal concentration of 100 mg/L within 3 h. Thus, it was separated into 12 fractions by the first-dimensional (D1) normal phase liquid chromatography (NPLC), meanwhile combining with in vitro antiparasitic tests for activity tracking. Subsequently, 8 flavonoids were identified in the active fractions by the second-dimensional (D2) reverse phase liquid chromatography (RPLC) tandem high-resolution mass spectrometry. According to the results, 5 flavonoids were selected for in vitro antiparasitic test, of which isobavachalcone showed the minimum parasiticidal concentration of 3.125 mg/L in 2 h. Bathing treatment of infected guppies with isobavachalcone could significantly reduce the burden of T. piriformis, obtaining a 24-h median effective concentration (24-h EC50) value of 1.916 mg/L. And the concentration of isobavachalcone causing guppies to die within 24 h is 39 times than that of 24-h EC50. The results demonstrated that isobavachalcone has the potential to be developed into a novel commercial fish drug against T. piriformis.

Enhanced immune response and resistance to edwardsiellosis following dietary chitooligosaccharide supplementation in the olive flounder (Paralichthys olivaceus).

This study was conducted to evaluate the effects of dietary chitooligosaccharide (COS) supplementation on peripheral leukocyte count, head kidney leukocyte phagocytic rate, phagocytic index, respiratory burst activity, serum lysozyme activity, and immune protection in Paralichthys olivaceus. A total of 300 flounder with an average body weight of 80-100 g were randomly assigned into four dietary groups: (I) basic diet (control), basic diet containing (II) 0.5% COS, and (III) 1% COS, fed continuously for 28 d, and (IV) basic diet containing 1% COS fed in 14 d intervals. Continuous feeding of 0.5% and 1% COS diets for 28 d significantly increased the number of peripheral leukocytes, head kidney leukocyte phagocytic rate, phagocytic index, respiratory burst activity, and serum lysozyme activity (P < 0.05 or P < 0.01). After a 10 d Edwardsiella tarda challenge, the immune protection rates in the 0.5% and 1% COS groups were 30% and 60%, respectively. No control fish survived the E. tarda challenge treatment. Most immune indices were slightly lower after removal of COS from the diet for 14 d, but all immune indices were observed to recover after another 14 d of COS supplementation. This study demonstrates that supplementation of a basic diet with COS enhances the non-specific immune response and improves survival rates following infection with E. tarda in P. olivaceus. An optimized interval feeding strategy with diets containing 1% COS may have potential applications in the prevention of disease in aquacultured P. olivaceus.