A CASE REPORT OF MAXILLARY LEIOMYOSARCOMA WITH REGIONAL LYMPH NODE METASTASIS

Studies on anticancer effect of momordin on oral carcinoma (KB) cells

Treatment of oral cancers with chemotherapeutic agents are evaluated as an effective method for remission to reduce cancer proliferation nowadays. But, minimization of side-effects such as bone marrow suppression, gastrointestinal toxicity and renal damage is another problem to be solved. Thus, a possible approach to develop a clinically applicable chemotherapeutic agents is to screen anticancer activity among traditional medicinal plants which have been used for thousands of years with very low side-effects in orient. In this study we focused on anti-oral cancer activities of momordin, which was medicinal plant extracts that was revealed anticancer activities, on KB cell(oral cancer cell). The results were as follow : 1. Momordin showed the excellent anti-oral cancer activity against KB cells. Obtained IC50 value of Momordin was 10.4 microgram/ml. 2. When KB cells were treated with Momordin, dose and time dependent DNA fragmentation of KB cells were observed. DNA fragmentation was initiated on three days at the concentration of 20 microgram/ml Momordin. 3. Flow cytometry showed dose-dependent apoptotic cell increase of KB cells on Momordin. 18.55% apoptotic cell were observed up to 72 hours at the concentration of 20 microgram/ml of Momordin 4. Momordin induced nonspecific apoptosis without specific cell cycle arrest. 5. Through MTT assay, DNA fragmentation assay and flow cytometric analysis. anticancer effect of Momordin against KB cell was induce of apoptotic cell death.

Studies on anti-oral cancer activities of medicinal plant extracts

Treatment of oral cancers with chemotherapeutic agents are evaluated as an effective method for remission to reduce cancer proliferation nowadays. But, minimization of side-effects such as bone marrow suppression, gastrointestinal toxicity and renal damage is another problem to be solved. Thus, a possible approach to develop a clinically applicable chemotherapeutic agents is to screen anticancer activity among traditional medicinal plants which have been used for thousands of years with very low side-effects in orient. In this study we focused on screening anti-oral cancer activities among 14 traditional medicinal plant extracts that revealed anticancer activities on other solid tumors. The results were as follow: 1. Methanol extract of Lepidium apetalum showed the highest anti-oral cancer activity against A253 cells. At concentration of 4 microgram/ml, the cell viability was 48% under our experimental condition. IC50 value obtained was 4 microgram/ml. 2. Methanol extract of Coptis japonica and Solanum nigrum were effective on KB cells. Cell viability observed were 62% and 67% at concentration of 4 microgram/ml, and IC50 values were 12 microgram/ml and 10 microgram/ml respectively. 3. When the methanol extract of Lonicera caerule was combined with 2 microgram/ml of cisplatin, the anticancer activity was synergistically increased. One hundred microgram/ml of Lonicera caerule showed 92% (alone) or 59% (combined with cisplatin) cell viabilities. IC50 value of Lonicera caerule extract against KB cells was reduced from 301 microgram/ml to 126 microgram/ml when combined with 2 microgram/ml of cisplatin. 4. Medicinal plant extracts effective on both A253 and KB cells were Coptis japonica, Lepidium apetalum, Solanum nigrum, Caesalpiniae Lignum, Curcuma aromatica.

The clinical effects of Nd: YAG laser application after extraction

Nd:YAG laser is well absorbed to water, melanin, hemoglobin and thus, Nd:YAG laser may be used for bleeding control and extraction site sterilization where is easily contaminated by saliva and blood. Additionaly, Nd:YAG laser have analgesic effect by elevation of pain threshold. On the basis of Nd:YAG laser effects, we applied the Nd:YAG laser on extraction socket of 50 patients who visited to our department for lower third molar extraction and evaluated the effects of Nd:YAG laser on the bleeding control, pain relief, swelling reduction after tooth extraction. For the objective assesment on Nd:YAG laser effects, we made up the other 50 patients(control group) who were treated by conventional extraction method and compare the subjective and objective symptoms(pain relief, swelling and oozing time) between each group. The results were as follows: 1. The Nd:YAG laser was effective to relief of postextraction pain and most effective to 2 hours after extraction when local anesthesia disappears. 2. The Nd:YAG laser application was non-effective to reducing the facial swelling after extraction. 3. The Nd:YAG was effective to decrease the oozing time after extraction and the average time was 4 hours.