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J Reprod Dev ; 54(1): 42-5, 2008 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-17984575

RESUMO

The present study was carried out to examine the activation and parthenogenetic development of pig oocytes after exposure to ultrasound in sorbitol media supplemented with different concentrations of Ca2+. The activation rates (68.8-75.6%) of oocytes exposed to ultrasound in media containing 0.1-1.0 mM Ca2+ were significantly (P<0.05) higher than those (54.3-58.3%) of oocytes exposed to ultrasound in media containing 0-0.05 mM Ca2+. When oocytes were exposed to ultrasound in media containing 0.1-0.5 mM Ca2+, the blastocyst formation rates (20.5-21.3%) were significantly (P<0.05) higher than those (3.3-6.0%) of oocytes exposed to ultrasound in media containing 0, 0.05 or 1.0 mM Ca2+. The results of the present study showed that the concentration of Ca2+ in the medium used for exposure to ultrasound affects the activation and parthenogenetic development of pig oocytes and showed that the optimal Ca2+ concentration is 0.1-0.5 mM.


Assuntos
Cálcio/farmacologia , Meios de Cultura/farmacologia , Oócitos/efeitos dos fármacos , Partenogênese/efeitos dos fármacos , Animais , Oócitos/diagnóstico por imagem , Oócitos/crescimento & desenvolvimento , Suínos , Ultrassonografia
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