RESUMO
During the search for secondary metabolites with antiproliferative activity, six new lanostane triterpenoids, tyrosamic acids A-F (1-6) together with ten known compounds (7-16), were isolated from the fruiting body of Tyromyces sambuceus. Their structures were elucidated using MS analyses, extensive 2D-heteronuclear NMR data interpretation and the structure of 3 was further confirmed by single-crystal X-ray data analyses. All lanostane triterpenoids (1-16) possesses a carboxy group at C-20 position and their strength of antiproliferative activity was affected by the presence or absence of a hydroxy group at C-15 position and at the side chain. Four of the compounds (1, 6, 10, 14) showed antiproliferative activities against human cancer cell lines with IC50 values of 16.8-48.3 µM (HL-60).
Assuntos
Antineoplásicos/farmacologia , Carpóforos/química , Polyporaceae/química , Triterpenos/química , Antineoplásicos/química , Linhagem Celular Tumoral , Humanos , Triterpenos/classificaçãoRESUMO
Two new phenanthrenes, and one new phenylpropanoid, named ephemeranthoquinone C (1), and marylaurencinols C (2) and D (3), were isolated from the roots of Cymbidium Great Flower 'Marylaurencin', respectively. These structures were determined on the basis of 2D NMR experiments. The compounds were tested for antimicrobial activity against Bacillus subtilis, Klebsiella pneumoniae, and Trichophyton rubrum.
Assuntos
Anti-Infecciosos/química , Orchidaceae/química , Fenantrenos/química , Fenilpropionatos/química , Anti-Infecciosos/isolamento & purificação , Anti-Infecciosos/farmacologia , Flores/química , Fenantrenos/isolamento & purificação , Fenantrenos/farmacologia , Fenilpropionatos/isolamento & purificação , Fenilpropionatos/farmacologia , Raízes de Plantas/química , Quinonas/químicaRESUMO
Two novel aromatic glucosides, named marylaurencinosides D (1) and E (2), were isolated from the fresh flowers of Cymbidium Great Flower 'Marylaurencin'. In addition, eight known aromatic compounds (3-10) were isolated. These structures were determined on the basis of NMR experiments as well as chemical evidence.
Assuntos
Glucosídeos/química , Orchidaceae/química , Flores/química , Glucosídeos/isolamento & purificaçãoRESUMO
Two new compounds named caryocanolide (1), and caryocanoside A (2), together with nine known compounds (3-11) were isolated from the whole plant of Caryopteris incana. These structures were determined mainly on the basis of 2D nuclear magnetic resonance and high resolution fast atom bombardment mass spectroscopy data. Furthermore, the isolated compounds (1, 2, 4-11) were tested for their antibacterial activity. Compound 1 exhibited moderate antimicrobial activity against Bacillus subtilis with a minimum inhibitory concentration value of 35.7 µM.
Assuntos
Antibacterianos/farmacologia , Extratos Vegetais/farmacologia , Verbenaceae , Antibacterianos/química , Antibacterianos/isolamento & purificação , Bacillus subtilis/efeitos dos fármacos , Bacillus subtilis/crescimento & desenvolvimento , Espectroscopia de Ressonância Magnética , Testes de Sensibilidade Microbiana , Modelos Moleculares , Estrutura Molecular , Fitoterapia , Extratos Vegetais/química , Extratos Vegetais/isolamento & purificação , Plantas Medicinais , Espectrometria de Massas de Bombardeamento Rápido de Átomos , Verbenaceae/químicaRESUMO
Two novel aromatic glucosides, named marylaurecinosides B (1) and C (2), were isolated from Cymbidium Great Flower Marie Laurencin, together with six known aromatic compounds (3-8). These structures were determined on the basis of NMR experiments as well as chemical evidence. All of the isolated compounds (1-8) were tested for antioxidative activity using a superoxide dismutase-like assay.
Assuntos
Antioxidantes/química , Flores/química , Orchidaceae/química , Espectroscopia de Ressonância Magnética , Estrutura Molecular , Fenantrenos/químicaRESUMO
A new lignan glycoside, 5-(3â³,4â³-dimethoxy-phenyl)-3-hydroxy-3-(4'-hydroxy-3'-methoxybenzyl)-4-hydroxymethyl-dihydrofuran-2-one 4'-O-α-L: -rhamnopyranoside (1), with seven known compounds, compound 2, koaburside, icariside E(4), cleomiscosin C, cleomiscosin D, scopoletin, and 5'-demethylaquillochin, were isolated from the EtOH extract of the wood of Acer saccharum (Aceraceae). Their structures were determined by 1D and 2D nuclear magnetic resonance (NMR) and mass spectroscopy analysis. All of the isolated compounds, 1-8, were tested for their antioxidant activity in superoxide dismutase (SOD)-like assay.
Assuntos
Acer/química , Antioxidantes/química , Glicosídeos/química , Lignanas/química , Antioxidantes/metabolismo , Glicosídeos/metabolismo , Lignanas/metabolismo , Espectroscopia de Ressonância Magnética , Estrutura Molecular , Superóxido Dismutase/metabolismoRESUMO
Four new resin glycosides, namely, ipomotaosides A-D (1-4), were isolated from the dried aerial parts of Ipomoea batatas. The structures of 1-4 were elucidated by analysis of their spectroscopic data and by chemical derivatization and were tested for their anti-inflammatory activity against cyclooxygenase (COX)-1 and -2.
Assuntos
Anti-Inflamatórios não Esteroides/isolamento & purificação , Anti-Inflamatórios não Esteroides/farmacologia , Inibidores de Ciclo-Oxigenase/isolamento & purificação , Inibidores de Ciclo-Oxigenase/farmacologia , Glicosídeos/isolamento & purificação , Glicosídeos/farmacologia , Ipomoea batatas/química , Plantas Medicinais/química , Anti-Inflamatórios não Esteroides/química , Ciclo-Oxigenase 1/efeitos dos fármacos , Ciclo-Oxigenase 2/efeitos dos fármacos , Inibidores de Ciclo-Oxigenase/química , Glicosídeos/química , Japão , Estrutura Molecular , Ressonância Magnética Nuclear Biomolecular , Resinas Vegetais/químicaRESUMO
A new aromatic derivative, 5-(3,4-dihydroxyphenyl)-3-methylcyclohex-2-enone (1), and three known compounds, peltigerine, solorinine, and phenyl butanoid, were isolated from the MeOH extract of the lichen Peltigera dolichorrhiza (Peltigeraceae). Their structures were determined by 1D and 2D nuclear magnetic resonance and mass spectroscopy analysis.
Assuntos
Líquens/química , Compostos Orgânicos/química , Aminoácidos/química , Guaiacol/análogos & derivados , Guaiacol/química , Espectroscopia de Ressonância Magnética , Espectrometria de Massas , Metanol/química , Estrutura MolecularRESUMO
From Cladonia rangiferina were isolated two novel abietane diterpenoids, hanagokenols A (1) and B (2). Also in this investigation, four known abitetane diterpenoids (3-6), four known labdane diterpenoids (7-10), one known isopimarane diterpenoid (11), and six known aromatic compounds were isolated. These structures were elucidated primarily through extensive NMR experiments. Hanagokenol A (1) was a unique abietane diterpene having an ether linkage between C-6 and C-18 of sugiol. Hanagokenol B (2) is also a unique secoabietane diterpene, having gamma-lactone which occurred by cleavage and subsequently oxidation between C-6/C-7 of 12-hydroxydehydroabietinol. Furthermore, all the isolated compounds (1-17) were tested for the antimicrobial activity against methicillin-resistant Staphylococcus aureus (MRSA) and vancomycin-resistant Enterococci (VRE).
Assuntos
Abietanos/isolamento & purificação , Abietanos/farmacologia , Antibacterianos/isolamento & purificação , Antibacterianos/farmacologia , Enterococcus/efeitos dos fármacos , Líquens/química , Plantas Medicinais/química , Staphylococcus aureus/efeitos dos fármacos , Abietanos/química , Antibacterianos/química , Diterpenos/química , Japão , Resistência a Meticilina , Testes de Sensibilidade Microbiana , Ressonância Magnética Nuclear Biomolecular , Resistência a VancomicinaRESUMO
Two new flavonoids quercetin 4'-O-alpha-L-rhamnopyranosyl-3-O-beta-D-allopyranoside (1) and apigenin 6-C-[2''-O-(E)-feruloyl- beta-D-glucopyranosyl]-8-C-beta-glucopyranoside (2), along with the known isorhamnetin 3-O-alpha-L-rhamnopyranoside (3), kaempferol 3-O-alpha-L-rhamnopyranosyl-(1-->4)-beta-D-glucopyranoside (4), and isovitexin (5) were isolated from the leaves of Acacia pennata Willd. (Mimosaceae) and tested for their anti-inflammatory activity. Their structures were determined by 1D and 2D NMR and mass spectrometry. They were tested for an inhibitory effect on COX-1 and COX-2, showing 60-90% inhibition at 10(-4) g/mL and 5-14% inhibition at 10(-4) g/mL, respectively.
Assuntos
Acacia , Ciclo-Oxigenase 1/efeitos dos fármacos , Ciclo-Oxigenase 2/efeitos dos fármacos , Inibidores de Ciclo-Oxigenase/farmacologia , Fitoterapia , Extratos Vegetais/farmacologia , Inibidores de Ciclo-Oxigenase/administração & dosagem , Inibidores de Ciclo-Oxigenase/uso terapêutico , Flavonas/administração & dosagem , Flavonas/farmacologia , Flavonas/uso terapêutico , Humanos , Concentração Inibidora 50 , Extratos Vegetais/administração & dosagem , Extratos Vegetais/uso terapêutico , Folhas de PlantaRESUMO
From the bark of Cryptomeria japonica were isolated sugikurojins I (1) and J (2), and an abietane derivative (3) was obtained for the first time as a natural product. These structures were elucidated primarily through extensive NMR experiments. Sugikurojin I (1) has a unique skeleton incorporating an abietane diterpene and a 1,10-secocadinane sesquiterpene. Sugikurojin J (2) is a peroxyester of hydroxyabietane diterpene and isopimarane acid diterpene. Compound 3 was p-quinone acid, which occurred by cleavage between C-7 and C-8 of sugiol; it was deduced to [4'-isopropyl-1(S),3,3-trimethyl-3',6'-dioxo-bicyclohexyl-1',4'-dien-2(R)-yl]-acetic acid. Also obtained in this investigation were three known diterpenes (4-6).
Assuntos
Abietanos/farmacologia , Antineoplásicos Fitogênicos/farmacologia , Cryptomeria/química , Casca de Planta/química , Abietanos/química , Abietanos/isolamento & purificação , Antineoplásicos Fitogênicos/química , Antineoplásicos Fitogênicos/isolamento & purificação , Espectroscopia de Ressonância Magnética , Estrutura Molecular , Plantas MedicinaisRESUMO
Three new diterpenes, sugikurojins A-C (1-3) were isolated from the black heartwood of Cryptomeria japonica. The structure of sugikurojin A was deduced to be 19-hydroxy-6,7-dehydroferruginol on the basis of extensive NMR experiments. Sugikurojin B was a dimer of 6,7-dihydroxyferruginol and 6,7-dehydroferruginol with a 6-O-11' linkage. Sugikurojin C was a dimeric ferruginol with 6-O-7' and 7-O-6' linkages. Also obtained in this investigation were the known compounds formosaninol (4), 15 sesquiterpenes (5-19), 16 diterpenes (20-35), three phenylpropanoids (36-38), and a phenolic compound (39).
Assuntos
Abietanos/isolamento & purificação , Cryptomeria/química , Plantas Medicinais/química , Abietanos/química , Japão , Estrutura Molecular , Ressonância Magnética Nuclear Biomolecular , Sesquiterpenos/química , Sesquiterpenos/isolamento & purificação , MadeiraRESUMO
Eight new lanostanoids, 12alpha,15beta-dihydroxy-3,7,11,23-tetraoxolanost-8,(20Z)(22)-dien-26-oic acid (1), 7beta,8beta-epoxy-15beta,20S-dihydroxy-3,12,23-trioxolanost-9(11),16-dien-26-oic acid (2), 7beta,8beta-epoxy-3beta,15beta,20S-trihydroxy-12,23-dioxolanost-9(11),16-dien-26-oic acid (3), 7beta,8beta-epoxy-2alpha,3beta,15beta,20S-tetrahydroxy-12,23-dioxolanost-9(11),16-dien-26-oic acid (4), 7beta,8beta-epoxy-3beta,15beta,20S,28-tetrahydroxy-12,23-dioxolanost-9(11),16-dien-26-oic acid (5), 7beta,8beta-epoxy-3beta,15beta,19,20S-tetrahydroxy-12,23-dioxolanost-9(11),16-dien-26-oic acid (6), 8beta,15beta,20S-trihydroxy-3,7,12,23-tetraoxolanost-9(11),16-dien-26-oic acid (7), 7alpha,8alpha-epoxy-15beta,23xi-dihydroxy-12-oxo-3,4-secolanost-4(28),9(11),(20Z)(22)-trien-3,26-dioic acid (8), and the methyl ester of 8 (8a) were isolated from the fruit bodies of Elfvingia applanata. Their structures were established primarily by NMR experiments, and their biological activity against Kato III and Ehlrich cells was investigated.