The effect of zinc oxide on rooster semen cryopreservation.

1. Deleterious effects from the freeze-thawing process on post-thawed sperm quality attributes are main limiting factors in cryopreservation. The current study was conducted to determine the effect of semen extender containing zinc oxide (ZnO) on post-thaw rooster sperm quality indices.2. Semen samples from six, 60-week-old broiler breeder roosters were collected weekly during five successive weeks. The samples were mixed and divided into three equal parts and diluted with semen extender containing different levels of ZnO; 0 (ZnO-0), 1 (ZnO-1) or 2 (ZnO-2) µg/ml. After thawing, motility and velocity parameters, plasma membrane functionality, apoptotic like changes, mitochondrial membrane potential (MMP), and DNA fragmentation index (DFI) were evaluated.3. Results showed that the addition of ZnO in the extender quadratically affected (P < 0.01) total motility (TM), progressive motility (PM), and average path velocity (VAP) with the highest values were noted in the ZnO-1 group. Levels of ZnO quadratically affected percentages of live (P < 0.01), apoptotic (P < 0.03) and dead (P < 0.10) spermatozoa, where the highest percentage of live, and the lowest percentage of apoptotic or dead spermatozoa was for the ZnO-1 group. Although adding ZnO quadratically affected plasma membrane functionality and MMP (P < 0.01), it did not affect (P > 0.05) DFI.4. In conclusion, there were some beneficial effects of ZnO supplementation in semen extender on post-thawed rooster sperm quality which may result in a better freezability.

Changes in broiler breeder hen's immunity by zinc oxide and phytase.

BACKGROUND: The immune response of aged broiler breeder hens is influenced by many factors including obesity and aged lymphatic organs, but may improve by increasing the bioavailability of various nutrients such as zinc (Zn). Dietary supplementation of phytase can improve Zn availability in senescent broiler breeder hens. AIMS: The aim of this study was to investigate the effect of supplementary zinc oxide (ZnO) and phytase in a maize-soybean meal-based diet on immune responses of broiler breeder hens. METHODS: In a 2 × 4 factorial arrangement, a total of 128 hens were randomly assigned into eight groups. The birds received two levels of phytase (0 or 300 U/kg diet) and four levels of ZnO (30, 60, 90, and 120 mg/kg diet) for 13 successive wk (59-72 wk of age). RESULTS: Results showed that phytase supplementation significantly increased immunoglobulin M (IgM), cutaneous basophil hypersensitivity (CBH) responses, total number of leukocytes, percentage of lymphocytes, and heterophil to lymphocyte ratios. The percentage of basophils and monocytes, however, decreased with phytase supplementation. Supplementation of ZnO increased anti-sheep red blood cells (SRBC) antibody titer, IgM, CBH responses, the total number of leukocytes, and the percentage of lymphocytes. Dietary supplementation of ZnO decreased the percentage of heterophil, and heterophil to lymphocyte ratio. A significant interaction effect of phytase and ZnO was found on the total number of leukocytes and percentage of lymphocytes. CONCLUSION: Dietary supplementation of ZnO (90 mg/kg diet) and phytase had some positive effects on improving immune responses in broiler breeder hens.

Effects of dietary curcumin supplementation on seminal quality indices and fertility rate in broiler breeder roosters.

1. Decreased semen quality is an underlying contributor to age-related subfertility in broiler breeder roosters. This study investigated the effects of dietary curcumin (derived from turmeric) supplementation as an antioxidant source on semen quality and fertility in broiler breeder roosters. 2. Twenty-eight Ross 308 roosters were randomly allotted to four groups with seven birds in each and were fed a standard diet supplemented with different levels of curcumin at 0 (C0), 10 (C10), 20 (C20) and 30 (C30) mg/bird per day from 48 through to 61 weeks of age. Body weight and semen quality traits were evaluated on a weekly basis and seminal concentrations of malondialdehyde (MDA) as a measure of antioxidation status were quantified at one-week intervals during the first 11 weeks of the trial (48-59 weeks of age). Semen samples from last 2 weeks (60 and 61 weeks of age) were used to artificially inseminate to assess the sperm-egg penetration (SP) in perivitelline membrane and fertility rates. 3. Except for body weight and ejaculate volume, other characteristics, including semen concentration, total sperm production, progressive motility and plasma membrane integrity were linearly improved by the increasing levels of curcumin supplementation (P < 0.01). However, dietary curcumin levels were linearly and quadratically associated with decreased seminal concentration of MDA (P < 0.01 and P < 0.03), percentage of abnormal sperm (P < 0.01 and P < 0.07) and increased plasma membrane functionality (P < 0.01 and P < 0.04), respectively. The SP holes in perivitelline membrane were increased in a linear and quadratic manner in response to increasing levels of curcumin (P < 0.01). Moreover, fertility rate was linearly improved (P < 0.01) as the dosage of curcumin increased, and resulted in 8, 12 and 14% improvements in the birds fed C10, C20 and C30, compared to C0, respectively. 4. In conclusion, the results showed that increasing levels of dietary supplementation of curcumin was associated with beneficial effects on semen quality indices and fertility rate in aged broiler breeder roosters.

First Report of Orobanche purpurea on Achillea wilhelmsii in Iran.

Achillea wilhelmsii C. Koch (Asteraceae) is a wild herb widely disturbed in Iran and known locally as yarrow. This plant is an important medicinal plant and it has been used for its analgesic, antiphlogistic, antispasmodic, and antibacterial effects in Iranian folk medicine. Field surveys revealed infestations of yarrow broomrape, Orobanche purpurea Jacq., on A. wilhelmsii plants in two locations in province of Zanjan (Zanjan, 36°37'19.85″ N, 48°27'16.87″ E, 1,707.4 m elevation, and Qolabar, 36°19'19.85″ N, 48°19'16.41″ E, 1,663.9 m elevation) in northwestern Iran in May 2012. The annual mean air temperature and the average maximum and minimum air temperatures were 11, 18, and 4°C, respectively. The annual mean precipitation was 298 mm. The infestation of A. wilhelmsii was confirmed by verifying the attachment of the yarrow broomrape to the A. wilhelmsii roots. Broomrape plant heights were between 24 and 37 cm. The stems were erect, unbranched, slender, and swollen near the base at contact with host, 0.5 to 10 mm diameter. The leaves were reduced to scale or bracts up to 10 to 15 mm long. The flowers were in dense terminal spikes, usually restricted to the top third or half of the shoot. There was one bract and two bracteoles surrounding each flower. The bracts measured 7 to 10 mm, had ovate lanceolates shorter than the calyces, and lanceolate bracteoles were 5 to 10 mm. The calyces were gamosepalous, entire, and whitish. Corollas were 13 to 18 mm long, and were dull bluish-purple with dark veins, suffused with whitish-yellow at the base. The upper part was slightly curved forwards. Stamens were epipetalous, inserted 4 to 7 mm above the corolla base, with filaments glabrous. The anthers were glabrous along sutures. Stigma lobes were white. Fruit was an oval capsule, 0.9 to 10 mm. Botanists at the College of Agriculture of Zanjan University confirmed the identity of O. purpurea. A. millefolium has been reported as a host plant for yarrow broomrape (1,2). However, to our knowledge, this is the first report of yarrow broomrape on A. wilhelmsii in Iran. Since production and farming of A. wilhelmsii as a medicinal plant has recently started on a commercial scale in Iran, the parasite weed could pose a serious threat to production of this plant. References: (1) B. Prajs. Biodiv. Res. Conserve. 17:33, 2010. (2) J. Rumsey and S. L. Jury. Watsonia. 18:257, 1991.