Quality markers of Dajianzhong decoction based on multicomponent qualitative and quantitative analysis combined with network pharmacology and chemometric analysis.

INTRODUCTION: Dajianzhong decoction (DJZD), a classic famous prescription, has a long history of medicinal application. Modern studies have demonstrated its clinical utility in the treatment of postoperative ileus (POI). But none of the current quality evaluation methods for this compound is associated with efficacy. OBJECTIVES: This study aimed to identify the quality markers (Q-Markers) connected to the treatment of POI in DJZD. METHODOLOGY: Ultra-performance liquid chromatography quadrupole Exactive Orbitrap mass spectrometry (UPLC-Q-Exactive Orbitrap-MS) was used to identify the main constituents in DJZD. Based on the qualitative results obtained by fingerprinting, chemical pattern recognition (CPR) was used to analyse the key components affecting the quality and finally to establish the network of the active ingredients in DJZD with POI. RESULTS: A total of 64 chemical components were detected. After fingerprint analysis, 13 common peaks were identified. The fingerprint similarity of 15 batches of samples ranged from 0.860 to 1.000. CPR analysis was able to categorically classify 15 batches of DJZD into two groups. And gingerenone A, methyl-6-gingerdiol, 6-gingerol, and hydroxy-ß-sanshool contributed to their grouping. Twelve common components interact with the therapeutic targets for treating POI. In addition, the mechanism of this prescription for treating POI may be related to the jurisdiction of the neurological system, the immunological system, and the inflammatory response. CONCLUSIONS: This integrated approach can accurately assess and forecast the quality of DJZD, presume the Q-Markers of DJZD for POI, and lay the foundation for studying the theoretical underpinnings and exploring the mechanism of DJZD in the treatment of POI.

Artemisia baimaensis allelopathy has a negative effect on the establishment of Elymus nutans artificial grassland in natural grassland.

Planting Elymus nutans artificial grassland to replace degraded Artemisia baimaensis grassland on the Qinghai Tibetan plateau (QTP) can effectively alleviate local grass-livestock imbalance. However, it is unknown whether the allelopathy of natural grassland plant A. baimaensis on E. nutans affects grassland establishment. Accordingly, we examined the effects of varying concentrations of aqueous extracts of A. baimaensis litter on the seed germination and early seedling growth of E. nutans, and the effects of A. baimaensis volatile organic compounds (VOCs) on the growth parameters and physiological characteristics of E. nutans. The results indicate that the aqueous extract inhibited the force, percentage, and index of germination of E. nutans and affected early seedling growth, particularly at high concentrations. Further, the VOCs significantly reduced the aboveground and root biomass of E. nutans and increased malondialdehyde concentrations. Additionally, these VOCs altered the antioxidant enzyme activities and increased the superoxide dismutase, peroxidase, ascorbic acid peroxidase, soluble sugar, and proline content but significantly decreased glutathione reductase levels. Our results indicate that the allelopathy of A. baimaensis significantly inhibited the germination and seedling growth of E. nutans . Thus, the leaching of A. baimaensis may produce allelochemicals in the soil that inhibit the germination of E. nutans seeds. Moreover, the VOCs of A. baimaensis may disrupt the growth process, resulting in a decrease in biomass and a disruption of the physiological metabolism of seedlings under field conditions.

Effect of Pheretima aspergillum on reducing fibrosis: A systematic review and meta-analysis.

Background: Pheretima aspergillum (common name: Earthworm, Chinese name: dilong) has been used in traditional Chinese medicine for thousands of years. Recently, a few scientific studies have investigated the antifibrotic effects of Dilong extract (DE) and produced controversial results. We conducted a meta-analysis to make an informed decision on the antifibrotic effects of Dilong extract. Methods: The studies on antifibrotic effects of Dilong extract published until July 2022 in the scientific databases [PubMed, Web of Science, China National Knowledge Infrastructure (CNKI), VIP database for Chinese Technical Periodicals, SinoMed and WanFang database] were reviewed. The RevMan 5.4.1 software was used for standardized mean difference (SMD) analysis. Two researchers independently reviewed all the studies, and their quality was assessed using the Cochrane risk of bias tool. Results: A total of 325 studies were found in the scientific databases; however, only 13 studies met the criteria for analysis. Dilong extract treatment was associated with antifibrotic effects via inhibiting the transforming growth factor beta 1 (TGF-ß1, SMD = -3.16, 95% CI: -4.18, -2.14, p < .00001) and alpha-smooth muscle actin (α-SMA: SMD = -2.57, 95% CI: -3.47, -1.66, p < .00001). Conclusion: Dilong extract effectively reduces tissue fibrosis; thus, further scientific studies should be conducted to investigate and develop it for clinical use. Systematic Review Registration: https://www.crd.york.ac.uk/prospero/, identifier CRD42022357141.

Highly Enantioselective Synthesis of Phosphorus-Containing ϵ-Benzosultams by Bifunctional Phosphonium Salt-Promoted Hydrophosphonylation.

ϵ-Benzosultam derivatives are potential drug candidates with diverse biological activities. A series of chiral ϵ-benzosultams bearing phosphorus functionalities was synthesized by catalytic asymmetric hydrophosphonylation in the presence of a bifunctional phosphonium salt catalyst. The desired hydrophosphonylation products were obtained in good yields with high enantioselectivities, and scale-up reactions and further derivations were successfully accomplished. Some control experiments were also conducted to elucidate the plausible reaction mechanism of this chemical transformation.

Identification of Motor and Mental Imagery EEG in Two and Multiclass Subject-Dependent Tasks Using Successive Decomposition Index.

The development of fast and robust brain-computer interface (BCI) systems requires non-complex and efficient computational tools. The modern procedures adopted for this purpose are complex which limits their use in practical applications. In this study, for the first time, and to the best of our knowledge, a successive decomposition index (SDI)-based feature extraction approach is utilized for the classification of motor and mental imagery electroencephalography (EEG) tasks. First of all, the public datasets IVa, IVb, and V from BCI competition III were denoised using multiscale principal analysis (MSPCA), and then a SDI feature was calculated corresponding to each trial of the data. Finally, six benchmark machine learning and neural network classifiers were used to evaluate the performance of the proposed method. All the experiments were performed for motor and mental imagery datasets in binary and multiclass applications using a 10-fold cross-validation method. Furthermore, computerized automatic detection of motor and mental imagery using SDI (CADMMI-SDI) is developed to describe the proposed approach practically. The experimental results suggest that the highest classification accuracy of 97.46% (Dataset IVa), 99.52% (Dataset IVb), and 99.33% (Dataset V) was obtained using feedforward neural network classifier. Moreover, a series of experiments, namely, statistical analysis, channels variation, classifier parameters variation, processed and unprocessed data, and computational complexity, were performed and it was concluded that SDI is robust for noise, and a non-complex and efficient biomarker for the development of fast and accurate motor and mental imagery BCI systems.

Polymeric nanofibrous scaffolds laden with cell-derived extracellular matrix for bone regeneration.

Bone tissue engineering aims to alleviate the shortage of available autograft material and the biological/mechanical incompatibility of allografts through fabrication of bioactive synthetic bone graft substitutes. However, these substitute grafting materials have insufficient biological potency that limits their clinical efficacy in regenerating large defects. Extracellular matrix, a natural tissue scaffold laden with biochemical and structural cues regulating cell adhesion and tissue morphogenesis, may be a versatile supplement that can extend its biological functionality to synthetic grafts. Embedding decellularized extracellular matrix (dECM) into synthetic polymers offers a promising strategy to enhance cellular response to synthetic materials, mitigate physical and mechanical limitations of dECMs, and improve clinical utility of synthetic bone grafts. Enriched with dECM biochemical cues, synthetic polymers can be readily fabricated into complex biocomposite grafts that mimic bone structure and stimulate endogenous cells to regenerate bone. In this study, cell-derived dECMs from osteoblast and endothelial cells were incorporated into polycaprolactone (PCL) solutions for electrospinning dual-layer nanofibrous scaffolds with osteogenic and vascular cues. The study examined the bioactivity of dECM scaffolds in osteoblast cultures for cell number, mineral deposits, and osteogenic markers, as well as regeneration of cortical bone defect in a rat femur. Scaffolds with osteoblast dECM had a significantly robust osteoblast proliferation, Alizarin Red staining/concentration, and osteopontin-positive extracellular deposits. Implanted scaffolds increased bone growth in femoral defects, and constructs with both osteogenic and vascular cues significantly improved cortical width. These findings demonstrate the potential to fabricate tailored biomimetic grafts with dECM cues and fibrous architecture for bone applications.

Polymeric ionically conductive composite matrices and electrical stimulation strategies for nerve regeneration: In vitro characterization.

Stem cell strategies and the use of electrical stimulation (ES) represent promising new frontiers for peripheral nerve regeneration. Composite matrices were fabricated by coating electrospun polycaprolactone/cellulose acetate micro-nanofibers with chitosan and ionically conductive (IC) polymers including, sulfonated polyaniline, and lignin sulfonate. These composite matrices were characterized for surface morphology, coating uniformity, ionic conductivity, and mechanical strength to explore as scaffold materials for nerve regeneration in conjunction with ES. Composite matrices measured conductivity in the range of 0.0049-0.0068 mS/m due to the uniform coating of sulfonated polymers on the micro-nanofibers. Thin films (2D) and composite fiber matrices (3D) of IC polymers seeded with human mesenchymal stem cells (hMSCs) were electrically stimulated at 0.5 V, 20 Hz for 1 h daily for 14 days to study the changes in cell viability, morphology, and expression of the neuronal-like phenotype. In vitro ES lead to changes in hMSCs' fibroblast morphology into elongated neurite-like structures with cell bodies for ES-treated and positive control growth factor-treated groups. Immunofluorescent staining revealed the presence of neuronal markers including ß3-tubulin, microtubule-associated protein 2, and nestin in response to ES. © 2018 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater 107B: 1792-1805, 2019.

Investigation of anaerobic side-stream phosphorus recovery and its effect on the performance of mainstream EBPR subjected to low-consumption.

In this study, phosphate-rich supernatant at the end of anaerobic phase was extracted by a certain side-stream ratio for chemical precipitation to investigate the optimal conditions for phosphorus recovery. The effect of side-stream reaction on the performance of the mainstream enhanced biological phosphorus removal (EBPR) system was also explored. The experiment was carried out in a sequencing batch reactor (SBR) operated in an alternating anaerobic/aerobic mode with dissolved oxygen controlled at 1.0 mg · L-1. The results showed that the optimum magnesium source,temperature, stirring speed and reaction equilibrium time for side-stream phosphorus recovery were: MgCl2 · 6H2O, 25 °C, 150 rpm and 20 min, respectively. It was also observed that the average phosphorus removal efficiency of the mainstream system maintained as high as 90.7% during the side-stream extraction period despite insufficient time for phosphate uptake under limited dissolved oxygen condition and phosphate deprivation of polyphosphate-accumulating organisms (PAOs). Besides, the sludge settling performance of the mainstream EBPR system decreased with no sludge loss. Afterwards, phosphorus removal and sludge settling performance were restored with dismissing side-stream phosphorus recovery. This study suggested that side-stream extraction of anaerobic supernatant from a mainstream EBPR subjected to low dissolved oxygen conditions for chemical phosphorus recovery was feasible and environmentally friendly.

[Effects of Long-term Side Stream Extracton on Phosphorus Removal and Recovery Performance of EBPR System].

A sequencing batch reactor (SBR) with alternating anaerobic/oxic (An/O) operation was employed to investigate nitrogen and phosphorus removal performance and corresponding phosphate recovery effect of an enhanced biological phosphorus removal (EBPR) system when extracting side stream ratios of 0, 1/4, 1/3, 1/2 anaerobic phosphorus supernatant. The operation involved three runs within 310 days. Removal efficiencies of COD and NH4+-N were found to be relatively stable over the entire experimental period and effluent could meet standard A of the national discharge standard of pollutants for municipal wastewater treatment plants. Total nitrogen removal improved due to enhancement of denitrification capability during the anaerobic phase, and the standard-reaching rate increased from 88.2% to 98.6%. Although phosphate releasing capability gradually decreased, phosphorus removal performances of the former two runs were stable and efficient. The removal rate was>90% and the corresponding standard-reaching rate was>75%. Nevertheless, phosphorus removal performance began to fluctuate with a side stream ratio of 1/2 during the third run. The lowest phosphorus removal rate was 54.2%, contributing to a 60% non-standard-reaching rate in this run. This suggests that long-term extraction of 1/2 side stream supernatant is not favorable for maintaining stable effluent of the main process. It was also found that long-term phosphate recovery through side stream extraction allowed reduction of sludge mass and had little impact on sludge settling performance. As a result, it is feasible to extract an appropriate side stream ratio of anaerobic supernatant to recover phosphate, combined with efficient removal of both nitrogen and phosphorus in the main process.

[Inhibitory Effect of Phenol on Phosphorus Removal Performance of an EBPR System].

An SBR reactor was operated to study the inhibitory effect of different concentrations of phenol (5, 10, 30, 50, 100, 150, and 200 mg·L-1) on the phosphorus removal performance of an EBPR system. The results showed that when the phenol concentration was no higher than 50 mg·L-1, the removal efficiencies of COD and PO43--P were above 85%. The system showed efficient pollutant removal performance. However, when phenol was higher than 100 mg·L-1, phosphorus removal performance of the reactor decreased drastically. When phenol concentration was 200 mg·L-1, the system lost phosphorus removal performance after only 22 cycles. The average COD removal efficiency dropped to 61.3%, and high removal performance recovery was not observed in the short term. Meanwhile, it was found that long-term dosing of phenol inhibited phosphorus removal of the sludge subjected to EBPR. Furthermore, the inhibition on aerobic phosphate uptake was greater than that on anaerobic phosphate release. In addition, the limited sludge bulking disappeared gradually owing to acclimation of the sludge to toxicity with a low phenol concentration (≤ 50 mg·L-1). However, high concentration of phenol induced sludge bulking was difficult to recover. The short-term impact experiments showed that effluent COD and phosphorus fluctuation caused by phenol could be recovered gradually by removing phenol, suggesting that the inhibitory effect of phenol on phosphorus removal was reversible.

[Phosphorus Removal Capacity of Domestic Wastewater Treated by a Modified CAST Process Under Different Operating Modes].

A modified cyclic activated sludge technology (CAST) treating domestic wastewater was employed to investigate the effects of different operating modes, such as C/P ratio, reflux ratio, and temperature on phosphorus removal. The results illustrated that at room temperature the phosphorus removal of the system improved significantly when the influent C/P ratio increased from 50 to 100, with the removal efficiency increasing rapidly from 15% to 95.6%. Decreasing the C/P ratio to 75, the phosphorus removal performance declined due to influent carbon source deficiency, and the mean phosphorous removal efficiency decreased to 51.4%. At the same time, the long-term addition of an easily degradable carbon source resulted in sludge bulking and a large amount of sludge loss. With a low C/P ratio, the phosphorus removal performance increased by 2.3 times when the sludge reflux ratio decreased from 25% to 12.5%. However, the phosphorus removal performance declined when reducing it to 0. The temperature experiment results showed that the phosphorous removal efficiency of the system remained stable, above 90%, in the low temperature system (14℃±1℃). However, the phosphorous removal efficiency of a shortcut nitrification system at high temperature (27℃±1℃) was only 14.1%, suggesting that a low temperature was beneficial for removing phosphorous. It was observed from the batch tests that the sludge at room temperature could utilize dissolved oxygen, nitrate, and nitrite as electron acceptors to take up phosphate. The sludge in the low temperature system could use both dissolved oxygen and nitrate as the electron acceptors. However, the sludge in the high temperature system could only use dissolved oxygen as the electron acceptors to take up phosphate. In addition, it was also found that sludge under starvation conditions resulting from short-term idling of the system favored phosphorus removal.

[Nitrite Type Denitrifying Phosphorus Removal Capacity of Cycle Activated Sludge Technology Processes Under Different Inducing Patterns].

A modified cyclic activated sludge technology (CAST) reactor was utilized to investigate the phosphorus and nitrogen removal performance under different inducing patterns in this experiment. The results show that nitrite addition under anoxic conditions has a more inhibitory effect on the denitrifying phosphorus removal performance of the sludge. The phosphorus removal performance of the system was least effective when nitrite dosage was 5 mg·L-1. Compared to an anoxic addition system, the CAST system is more stable under aerobic addition conditions. The phosphorus removal properties have a slight fluctuation during each initial operating condition when the nitrite concentrations are 5, 10 and 15 mg·L-1, respectively. However, the phosphorus removal rate was observed to recover quickly and remain stable at more than 95% after acclimatizing for 10, 6, and 34 days, respectively. The effluent phosphorus concentration was less than 0.5 mg·L-1 in all cases. It was also found that the phosphorus removal performance deteriorated drastically when the nitrite dosage was 20 mg·L-1. Nevertheless, the nitrite type denitrifying phosphorus uptake capacity of the sludge was 10.4 times greater than that of the sludge before acclimatizing, suggesting that the phosphorus performance deterioration due to nitrite addition could be relieved and long-term addition is beneficial to enriching denitrifying phosphorus accumulating bacteria using NO2- as an electron acceptor. Moreover, the sludge settling performance was found to be effective and the sludge concentration decreased continuously when adding a certain concentration of nitrite under aerobic conditions, which is of significant for sludge reduction.

[Effects of Side-stream Phosphorus Recovery on the Performance of EBPR System Under Low Dissolved Oxygen Condition].

A sequencing batch reactor (SBR) was operated with alternating anaerobic/oxic (An/O) condition to investigate the nitrogen and phosphorus removal performance of EBPR with DO=1 mg·L-1 and the phosphorus recovery effect at extracting side stream ratio of 0, 1/4, 1/3, 1/2 anaerobic phosphorus supernatant. The removal efficiency of NH4+-N was found to be relatively stable during all experimental stages but effluent COD of the system during later period remained as high as 81.3 mg·L-1. In the absence of side stream, the average phosphorus removal efficiency was 89.4% and increased to 98.5% and 99.0% when the side stream ratio was 1/4 and 1/3, respectively. However, the phosphorus removal performance began to fluctuate with side stream ratio of 1/2 and the lowest phosphorus removal rate was 65.4%. During each experimental stage, only one day effluent TP failed to meet the national wastewater discharge standard A with side stream ratio of 1/3 in the initial period. Besides, 93.3% of effluent TP which could meet the national standard with side stream ratio of 1/3 was far better than 45.5% of that with side stream ratio of 1/2. Nevertheless, the phosphorus recovery rate improved with the increase of side stream ratio. It was also observed that the TN removal rate dramatically decreased to 50.9% when the side stream ratio increased to 1/2. Consequently, a side stream ratio of 1/3 was found to be the optimal condition in the whole experimental stage, and EBPR system under low dissolved oxygen condition combined with phosphorus recovery through extracting side stream could greatly improve the economic and environmental benefits.

[Phosphorus Removal Performance in EBPR System under Extra-low Dissolved Oxygen Condition].

Two sequencing batch reactors (SBRs) were operated with alternating anaerobic/oxic (An/O) for 127 days to study the phosphorus removal efficiency and process performance of the EBPR under different dissolved oxygen gradient. For the reactor (R1) in which DO was not controlled and measured as high as 6 mg·L-1 during the aerobic stage, a stable phosphorus removal performance was achieved in the former 65 days during the operational period. The phosphorus removal efficiency was greater than 95.9% and the total phosphorus in effluent was less than 0.5 mg·L-1. However, the phosphorus removal performance subsequently deteriorated after 65 days and completely broke up until 97 day due to long-term excessive aeration. Throughout the operational period,only 39.4% of effluent TP met the national wastewater discharge standard A. For the test reactor (R2) in which DO was controlled at the levels of 2, 1, 0.5, 0.2, 0.1 mg·L-1 during the aerobic stage, respectively, the phosphorus removal performance showed a slight fluctuation at the beginning of each stage but rapidly increased to a stable state. According to the statistics, 94.6% of effluent TP in reactor R2 could meet the national wastewater discharge standard A throughout the operational period. In only 6 days out of the 127 days, effluent TP failed to meet the national standard and the process performance was far better than that of R1. The specific phosphate uptake rate under DO=2 mg·L-1 was found to be nearly the maximum, but it was greatly influenced by the low DO. In addition, limited filamentous bulking resulted from low organic matter degradation rate under extra-low DO level (0.1 mg·L-1) was observed. Nevertheless, 100% of effluent TP during this stage could meet the national standard. As a result, the phosphorus removal in micro-aerobic EBPR system for saving energy was feasible.

[Startup, stable operation and process failure of EBPR system under the low temperature and low dissolved oxygen condition].

A sequencing batch reactor (SBR) was started up and operated with alternating anaerobic/oxic (An/O) to perform enhanced biological phosphorus removal (EBPR) under the condition of 13-16 degrees C. The results showed that under the condition of low temperature, the EBPR system was successfully started up in a short time (<6 d). The reactor achieved a high and stable phosphorus removal performance with an influent phosphate concentration of 20 mg x L(-1) and the dissolved oxygen (DO) concentration of 2 mg x L(-1). The effluent phosphate concentration was lower than 0.5 mg x L(-1). It was found that decreasing DO had an influence on the steady operation of EBPR system. As DO concentration of aerobic phase decreased from 2 mg x L(-1) to 1 mg x L(-1), the system could still perform EBPR and the phosphorus removal efficiency was greater than 97.4%. However, the amount of phosphate released during anaerobic phase was observed to decrease slightly compared with that of 2 mg x L(-1) DO condition. Moreover, the phosphorus removal performance of the system deteriorated immediately and the effluent phosphate concentration couldn't meet the national integrated wastewater discharge standard when DO concentration was further lowered to 0.5 mg x L(-1). The experiments of increasing DO to recover phosphorus removal performance of the EBPR suggested the process failure resulted from low DO was not reversible in the short-term. It was also found that the batch tests of anoxic phosphorus uptake using nitrite and nitrate as electron acceptors had an impact on the stable operation of EBPR system, whereas the resulting negative influence could be recovered within 6 cycles. In addition, the mixed liquid suspended solids (MLSS) of the EBPR system remained stable and the sludge volume index (SVI) decreased to a certain extend in a long run, implying long-term low temperature and low DO condition favored the sludge sedimentation.

Trace Element Concentrations in Human Tissues of Death Cases Associated With Secondary Infection and MOF After Severe Trauma.

Proper trace element level is crucial for the organs in maintaining normal physiological functions. Multiple organ failure (MOF) might be added to critically ill patients due to a lack of trace elements. Alterations of trace element levels in brain, heart, liver, and kidney after severe trauma, however, have been little studied so far. In this study, tissue samples of the frontal cortex of the brain, interventricular septum of the heart, right lobe of the liver, and upper pole of the kidney were obtained from forensic autopsies, of which 120 cases died during the 5th to 15th day of hospitalization, whereas the trauma death group and 43 cases immediately died due to severe craniocerebral trauma as the control group. Copper (Cu), iron (Fe), zinc (Zn), and selenium (Se) were quantified by inductively coupled plasma atomic emission spectrophotometry (ICP-AES). Cu, Fe, Zn, and Se concentrations in the brain, heart, liver, and kidney in the trauma group decreased dramatically (p<0.05) compared to the control group. The incidence of secondary infection and multiple organ failure (MOF) in the trauma death group were 78.33 and 29.17%, respectively. The concentrations of all elements exhibited a significant correlation with secondary infection and MOF (p<0.01). Our data suggest that low concentrations of Cu, Fe, Zn, and Se in pivotal organs may contribute to the incidence of secondary infection and MOF after severe trauma, which to some extent results in death.

Analysis of blood trace elements and biochemical indexes levels in severe craniocerebral trauma adults with Glasgow Coma Scale and injury severity score.

We aimed to investigate the correlation between the Glasgow Coma Scale (GCS), the injury severity score (ISS) and serum levels of trace elements (TE) in severe trauma patients to analyze alteration of the levels of trace elements and serum biochemical indexes in the period of admission from 126 adult cases of severe brain trauma with traffic accidents. Multi-trace elements for patients in the trauma-TE groups were used. The results indicated that all patients presented an acute trace elements deficiency syndrome (ATEDs) after severe trauma, and the correlation between ISS and serum levels of Fe, Zn, and Mg was significant. Compared to the normal control group, levels of the trace elements in serum were significantly decreased after trauma, suggesting that enhancement of immunity to infection and multiple organ failure (MOF) via the monitoring and supplement of trace elements will be a good strategy to severe traumatic patients in clinics.

[Long-Term Inhibition of FNA on Aerobic Phosphate Uptake and Variation of Phosphorus Uptake Properties of the Sludge].

An alternating anaerobic/oxic ( An/O) sequencing batch reactor (SBR) was employed to investigate the long-term inhibitory effect of free nitrous acid (FNA) on aerobic phosphorus uptake performance and variation of phosphorus uptake properties of the sludge by adding nitrite. The reactor was started up under the condition of 21-23 degrees C. The results showed that FNA had no impact on phosphate release and uptake capacities of the sludge. However, the specific phosphate release/uptake rates was found to be higher. As FNA concentration (measure by HNO2-N) was lower than 0.53 x 10(-3) mg x L(-1), phosphorus removal efficiency of the system was higher than 96.9%. When the FNA concentration was increased to 0.99 x 10(-3) mg x L(-1), 1.46 x 10(-3) mg x L(-1) and 1.94 x 10(-3) mg x L(-1), the phosphorus removal performance deteriorated rapidly. The phosphorus removal efficiency was recovered to 64.42%, 67.33% and 44.14% after 50, 12 and 30 days, respectively, which implied the deterioration of phosphorus removal performance caused by FNA inhibition could be recovered and long-term acclimation could shorten the recovery process. Notably, increasing nitrite consumption appeared during aerobic phase with the concentration of FNA below 1.46 x 10(-3) mg x L(-1). It was also observed that the phosphorus uptake properties of the sludge varied after long-term inhibition. Nitrate and nitrite type anoxic phosphorus uptake capacity was increased by 3.35 and 3.86 times, respectively, suggesting long-term dosing FNA may facilitate the denitrifying of polyphosphate in organisms utilizing nitrite as electron acceptor. Moreover, long-term acclimation favored sludge settling.

Peripheral Nerve Regeneration Strategies: Electrically Stimulating Polymer Based Nerve Growth Conduits.

Treatment of large peripheral nerve damages ranges from the use of an autologous nerve graft to a synthetic nerve growth conduit. Biological grafts, in spite of many merits, show several limitations in terms of availability and donor site morbidity, and outcomes are suboptimal due to fascicle mismatch, scarring, and fibrosis. Tissue engineered nerve graft substitutes utilize polymeric conduits in conjunction with cues both chemical and physical, cells alone and or in combination. The chemical and physical cues delivered through polymeric conduits play an important role and drive tissue regeneration. Electrical stimulation (ES) has been applied toward the repair and regeneration of various tissues such as muscle, tendon, nerve, and articular tissue both in laboratory and clinical settings. The underlying mechanisms that regulate cellular activities such as cell adhesion, proliferation, cell migration, protein production, and tissue regeneration following ES is not fully understood. Polymeric constructs that can carry the electrical stimulation along the length of the scaffold have been developed and characterized for possible nerve regeneration applications. We discuss the use of electrically conductive polymers and associated cell interaction, biocompatibility, tissue regeneration, and recent basic research for nerve regeneration. In conclusion, a multifunctional combinatorial device comprised of biomaterial, structural, functional, cellular, and molecular aspects may be the best way forward for effective peripheral nerve regeneration.

Blood zinc, iron, and copper levels in critically ill neonates.

The aim of this study is to explore the prognostic value of blood zinc, iron, and copper levels in critically ill neonates by comparing blood metal levels with the score for neonatal acute physiology (SNAP). Forty-six neonates (26 boys, 20 girls; ages ranging from 10 min to 23 days) who had been admitted to the neonatal intensive care unit of hospital and who were critically ill according to SNAP were included. Another 15 neonates (12 boys, 8 girls; ages ranging from 30 min to 24 days) who were brought to the hospital for a health checkup were included as controls. Clinical data, time in the intensive care unit, prognosis, and SNAP for critically ill neonates were recorded. Blood Cu, Zn, and Fe values were measured by inductively coupled plasma atomic emission spectrophotometry. Ill neonates were divided into extremely critical (SNAP ≥ 10) and critical groups (1 ≤ SNAP < 9). Zn levels were lower in patients than in controls (p <0.05). Cu levels did not differ between patients and controls (p >0.05). Fe levels were not significantly between the critical and control groups (p >0.05). In ill neonates, blood Zn and Fe concentrations in the extremely critical group were lower than in the critical group (p <0.05). Serious illness in neonates may lead to decreased Zn and Fe blood concentrations. Zn and Fe supplements may be beneficial for critically ill children.