Hydrodynamic behavior and start-up performance of a periodic anaerobic baffled reactor in an "every second" switching manner treating traditional Chinese medicine wastewater.

Most studies focus on the "clockwise sequential" switching manner for a four-compartment periodic anaerobic baffled reactor (PABR), while the exploration of the "every second" option on the feasibility for real industrial wastewater treatment is rarely reported. Hence, a PABR-treating traditional Chinese medicine wastewater was run continuously in "every second" switching manner with both switching period T and hydraulic residence time of 48 h. Satisfactory start-up performance was achieved during the operation of a climbing average organic load rate at approximately 1, 2, 4, and 6 kg chemical oxygen demand (COD) m-3 d-1 for 12, 24, 24, and 6 days, respectively. The average COD removal was 87.20% after the second lifting of OLR and 89.98% after the third one. Denaturing gradient gel electrophoresis and its cluster analysis showed that the microbial communities in each compartment adapted their structure in response to the periodically changing micro-ecology conditions. Moreover, the residence time distribution test with tap water in the clean PABR was carried out in experiments and computational fluid dynamics (CFD) simulation, both of which were in good agreement. The CFD model output visualized the flow velocity field and hydrodynamic-mass transport inside the PABR. Optimization of operation pattern in PABR including switching manner and frequency depended on both the type of waste being treated and the flexibility of biomass to periodically changing micro-ecology conditions.

Startup and stable operation of advanced continuous flow reactor and the changes of microbial communities in aerobic granular sludge.

In this study, the granular sludge was operated under low aeration condition in sequencing batch reactor (SBR) and advanced continuous flow reactor (ACFR), respectively. Through increasing the sludge retention time (SRT) from 22 days to 33 days, the ACFR was successful startup in 30 days and achieved long term stable operation. Under SBR operation condition, the aerobic granular sludge (AGS) showed good nitrogen (60%), phosphorus (96%) and COD removal performance. During stable operation of continuous-flow, the nitrogen removal efficiency was increasing to 70%, however, the phosphorus removal efficiency could only be restored to 65%. Meanwhile, the sludge discharge volume from ACFR was about half of that in SBR. Results of high-throughput pyrosequencing illustrated that methanogenic archaea (MA), ammonia oxidizing archaea (AOA), denitrifying bacteria (DNB), denitrifying polyphosphate-accumulating organisms (DPAOs) played an important role in the removal of nutrients in ACFR. This study could have positive effect on the practical application of AGS continuous flow process for simultaneous biological nutrient removal (SBNR).

Effect of different operational modes on the performance of granular sludge in continuous-flow systems and the successions of microbial communities.

Continuous flow reactors with time intermittent operational (TIO) mode and spatial intermittent operational (SIO) mode were operated to evaluate the effects of operational modes on the removal performances, the characteristics of granules and the dynamics of microbial communities in simultaneous nitrification, denitrification and phosphorus removal (SNDPR) granular system. The results showed that the removal efficiency of TP, TN were 81.3%, 86.7% under TIO mode, and 70.6%, 77.4% under SIO mode, respectively. Meanwhile, the PN and value of PN/PS in SIO were higher than those in TIO. Besides, results of high-throughput pyrosequencing illustrated that the combination of filamentous archaea (Methanothrix) and filamentous bacteria (Thiothrix) had resulted in the increase of EPS and SVI under SIO mode. Finally, functional bacterial and archaeal species, involving HMA, AMA, AOA, DPAOs etc., were identified to reveal the effects of operational modes on the mechanism of nutrients removal in granular SNDPR continuous-flow system.

Effect of aeration modes on simultaneous nitrogen and phosphorus removal and microbial community in a continuous flow reactor with granules.

In this study, a continuous flow reactor with simultaneous nitrification, denitrification and phosphorus removal (SNDPR) granular sludge was operated in the continuous aeration (CA) and intermittent aeration (IA) modes to examine the effect of aeration on the performance of continuous-flow system. Then the experimental results showed that the IA1 mode (4 h aeration and 1 h non-aeration) could improve the simultaneous nitrogen and phosphorus removal and the settleability of granules in continuous flow system. Results of high-throughput pyrosequencing illustrated that the methanogens, AOA, ANAMMOX, DNB, denitrifying polyphosphate-accumulating organisms (DPAOs) were the important participant of simultaneous biological nutrients removal (SBNR), meanwhile, the IA1 mode could effectively inhibit the growth of filamentous microorganisms (Thiothrix and Acinetobacter). Finally, a conceptual model of the SNDPR granular microbial ecosystem under IA1 mode was proposed as a base for analyzing the mechanism of simultaneous nutrient removal in continuous flow system.

Performance of a periodic anaerobic baffled reactor fed on Chinese traditional medicine industrial wastewater.

A four-compartment periodic anaerobic baffled reactor (PABR) was run in a 'clockwise sequential' switching manner continuously fed on chinese traditional medicine industrial wastewater under an alkalinity concentration between 1000 and 1500 mg CaCO(3)/L of the feed with average organic load rate (OLR) at about 1, 2, 4 and 6 kg COD/(m(3)d) for 12, 24, 24 and 6d, respectively. Hydraulic residence time was 2d, while switching period was 4d. As the average OLR increased to 6 kg COD/(m(3)d), the time of the sharp fall in pH, chemical oxygen demand (COD) removal, gas production and methane percentage of the biogas of all the compartments and the time of rapid volatile fatty acids accumulation in effluent coincided, hence the PABR became sour. Denaturing gradient gel electrophoresis (DGGE) community fingerprints and their cluster analysis revealed that community structures of each compartment tended to be more closely related if the PABR was not overloaded.