RESUMO
The experiments on rats showed that severe compression trauma of limb muscles leads to a decrease in the energetic potential of the liver. Cytoflavine injected in rats with model traumas increased the content of ATP and cAMP and decreased the level of ADP and AMP, thus recovering the energetic potential of adenine nucleotides in the liver.
Assuntos
Difosfato de Adenosina/metabolismo , Trifosfato de Adenosina/metabolismo , Metabolismo Energético/efeitos dos fármacos , Mononucleotídeo de Flavina/farmacologia , Membro Posterior/lesões , Inosina Difosfato/farmacologia , Fígado/metabolismo , Músculo Esquelético/lesões , Niacinamida/farmacologia , Succinatos/farmacologia , Animais , Combinação de Medicamentos , Fígado/patologia , Masculino , Músculo Esquelético/metabolismo , Músculo Esquelético/patologia , Ratos , Índices de Gravidade do Trauma , Ferimentos e Lesões/tratamento farmacológico , Ferimentos e Lesões/metabolismoRESUMO
Experiments were conducted on rats to study the antioxidant effects of amtisol and trimethasidin (25 mg/kg) in incomplete cerebral ischemia complicated by hypoxia. Amtisol as well as trimethasidin inhibited activation of lipid peroxidation (LPO) and prevented decrease in activity of the antioxidant system. In in vitro experiments amtisol and trimethasidin blocked epinephrine auto-oxidation. Amtisol inhibited ascorbate-dependent LPO in the liposomes and metabolizing model system, inhibited fermentative NADP-dependent POL in brain tissue homogenates.
Assuntos
Antioxidantes/uso terapêutico , Tiadiazóis/uso terapêutico , Trimetazidina/uso terapêutico , Doença Aguda , Animais , Antioxidantes/metabolismo , Câmaras de Exposição Atmosférica , Encéfalo/efeitos dos fármacos , Encéfalo/metabolismo , Modelos Animais de Doenças , Avaliação Pré-Clínica de Medicamentos , Epinefrina/metabolismo , Hipóxia-Isquemia Encefálica/tratamento farmacológico , Hipóxia-Isquemia Encefálica/metabolismo , Técnicas In Vitro , Peroxidação de Lipídeos/efeitos dos fármacos , Masculino , Oxirredução/efeitos dos fármacos , RatosRESUMO
Experiments were conducted on rats to study the effect of amtizol and trimetazidine on energy metabolism and lipid peroxidation in the brain, heart, and liver in acute hypoxia. Hypoxia was modelled by "raising" the animals in a pressure chamber to a "height" of 8,000-11,000 m, the exposure lasted 30 min. Equal doses (25 mg/kg) of the drugs were injected intraperitoneally 30 min before the "ascent". It was found that amtizol, just like trimetazidine, prevented energy metabolism disorders and activation of lipid peroxidation in the organs. The metabolic effects of the drugs were unidirectional, but the antioxidant activity of amtizol was pronounced more.
Assuntos
Hipóxia/tratamento farmacológico , Tiadiazóis/uso terapêutico , Trimetazidina/uso terapêutico , Doença Aguda , Altitude , Análise de Variância , Animais , Relação Dose-Resposta a Droga , Avaliação Pré-Clínica de Medicamentos , Metabolismo Energético/efeitos dos fármacos , Hipóxia/metabolismo , Peroxidação de Lipídeos/efeitos dos fármacos , Masculino , RatosRESUMO
The effect of amthizol on energy metabolism and lipid peroxidation in the brain, heart, liver, kidneys, and skeletal muscles was studied in experiments on rats with acute hypoxia (altitude 8,000 m, exposure 30 min). Intraperitoneal injection of amthizol (25 mg/kg) 30 min before the development of hypoxia prevented disorders of energy metabolism in the organs and inhibited activation of lipid peroxidation.
Assuntos
Metabolismo Energético/efeitos dos fármacos , Hipóxia/tratamento farmacológico , Peroxidação de Lipídeos/efeitos dos fármacos , Tiadiazóis/farmacologia , Doença Aguda , Animais , Câmaras de Exposição Atmosférica , Avaliação Pré-Clínica de Medicamentos , Hipóxia/metabolismo , Masculino , Ratos , Tiadiazóis/uso terapêuticoRESUMO
After severe mechanical trauma of rat soft tissue content of ATP, total adenine nucleotides, amounts of "energy charge" of the adenine nucleotide system and cytochrome c were decreased in liver tissue, while content of AMP and Pi was increased. Parenteral administration of cytochrome c at a dose of 10 mg/kg into animals after the trauma normalized the energy processes in liver tissue and increased the content of endogenous cytochrome c.