RESUMO
BACKGROUND: Work has shown that cutaneous microbial species associated with skin conditions of microbial aetiology are susceptible to killing by antimicrobial photodynamic therapy (APDT) using visible light and methylene blue. OBJECTIVES: To evaluate immediate and delayed genotoxicity of APDT on keratinocytes in vitro. METHODS: A combination of methylene blue (100 microg mL(-1)) and visible light (42 mW cm(-2)), as used in studies of microbe and keratinocyte cytotoxicity, was employed to test a human keratinocyte cell line (H103) for genotoxic damage by comet assay. RESULTS: The comet assay was able to detect genotoxic damage in H2O2-treated keratinocytes (positive control). APDT did not cause either immediate or delayed genotoxic damage in keratinocytes following APDT of up to 180 min. CONCLUSIONS: APDT sufficient to reduce microbes by seven log cycles showed no detectable genotoxic effects on keratinocytes. APDT applied in vivo may represent a useful low-risk alternative to conventional antimicrobial treatment in dermatology.
Assuntos
Queratinócitos/efeitos da radiação , Fototerapia/efeitos adversos , Linhagem Celular , Ensaio Cometa/métodos , Dano ao DNA/genética , Humanos , Peróxido de Hidrogênio/farmacologia , Queratinócitos/efeitos dos fármacos , Queratinócitos/microbiologia , Azul de Metileno/farmacologia , Dermatopatias Infecciosas/tratamento farmacológico , Dermatopatias Infecciosas/genéticaRESUMO
In this paper, we assess the antiviral properties of honey solutions and thyme extracts at varying concentrations. This was done by testing these solutions in vitro using monkey kidney cell cultures that were infected with the Rubella virus. Our results indicated that honey had good anti-Rubella activity, while thyme did not. These results may justify the continuing use of honey in traditional medicines from different ethnic communities worldwide and in some modern medications such as cough syrups.