RESUMO
An investigation on seeds of Brucea javanica led to the acquisition of a new quassinoid, 20-hydroxyyadanzigan (1), along with five known quassinoids (2-6). The structure of the new compound was elucidated on the basis of extensive spectral analysis. All of the compounds were assayed for their anticomplement activities through classical and alternative pathways. Compounds 1-6 exhibited potent anticomplement activity with CH50 and AP50 values of 0.032-0.075 mg/mL and 0.061-0.118 mg/mL, respectively. Moreover, the structure-activity relationships of these compounds are discussed.[Formula: see text].
Assuntos
Brucea/química , Proteínas Inativadoras do Complemento/farmacologia , Quassinas/isolamento & purificação , Sementes/química , Estrutura Molecular , Extratos Vegetais/química , Relação Estrutura-AtividadeRESUMO
A new polyhydroxylated pregnane, named lß,2ß,3ß,4ß,5ß,6ß-hexolhydroxy-pregn-16-en-20-one (1), along with nine known (2-10) steroidal saponins were isolated from the whole plant of Reineckia carnea. Structure elucidations of all compounds were established by interpretation of their NMR spectral data, HR-ESI-MS and comparing with literatures. In addition, these compounds were evaluated with anticomplement activity. The result showed that compound 1 exhibited anticomplement effects with the CH50 values of 0.043 mg/mL, but saponins (2-10) showed no inhibition. Interestingly, hydrolysis of steroidal saponins (2-10) resulted in its aglycones (2a-10a) correspondingly which showed anticomplement activity with the CH50 values of 0.049-0.156 mg/mL.