RESUMO
Objective: This study aims to investigate and analyze the detection rate of breast cancer utilizing color Doppler ultrasonography in asymptomatic individuals. Methods: A cohort of 500 female patients who underwent physical examinations at our hospital from January 2018 to August 2020 was selected for this research. The diagnosis involved conventional surgical palpation and color Doppler ultrasound in all cases. The control group underwent conventional surgical palpation, while the study group utilized the color Doppler ultrasound diagnosis method. A comparative analysis of the results obtained by both methods was conducted, involving observation and recording of all detected objects. Results: The study group demonstrated a significantly higher detection rate of hyperplasia (40.20%) and nodules (19.00%) compared to the control group (32.60%, 12.40%) (P < .05). Biopsy or surgical resection of tissue samples from 200 subjects revealed nine cases of breast cancer and 191 cases without breast cancer. In comparison to pathological examination, color Doppler ultrasonography exhibited increased accuracy (95.50%) and sensitivity (100.00%) with a specificity of 95.29%. Analysis of color Doppler ultrasound images from five subjects demonstrated its intuitive and effective use in diagnosing breast mass characteristics and understanding tissue morphology, edges, and size. Conclusions: The application of color Doppler ultrasound in the physical examination of healthy individuals proves effective in breast cancer screening. It provides a clear display of breast disease symptoms, offering an essential imaging foundation for cancer diagnosis. Clinically feasible, it is recommended as a routine examination method for widespread use across the clinical spectrum.
RESUMO
According to the genomic sequence of foreign four PLRV isolates, three pairs of specific primer were designed and synthesized. The cDNA of the ORF2a gene of PLRV-Ch was synthesized by reverse transcription and followed by Polymerase Chain Reaction amplication. The synthesized 3' and 5' cDNA fragment of the PLRV-Ch ORF2a gene were inserted into pUC19 and cloned in E. coli JM109 and were sequenced respectively. The middle cDNA fragment were directly sequenced. The homology of nucleotide sequence of PLRV-Ch compared with PLRV-S (Scotland, UK), PLRV-N(Netherlands), PLRV-A(Australia) and PLRV-C(Canada) were 98.96%, 98.70%, 94.79%, 97.5%, the homology of putative amino acid sequence are 97.97%, 97.97%, 89.69%, 95.94%. In 3' region of ORF2a gene a slippery sequence for-1 frameshift and its downstream "stem-loop" or "pseudoknot" and upstream nucleotide sequence repeats were found. Authors suggested that the nucleotide repeat sequences characteristic for PLRV could form a tight successively folded complementary double stranded regions and hairpins. This structure possibly has something to do with-1 frameshift. The amino acid sequence of C terminus region of 70 kD protein translated by motif IV has a protease characteristic motif and a helicase motif IV. The amino acid sequence of polypeptide translated by ORF2a gene undergoing frameshift has a single-stranded nucleic acid binding protein-like characteristic motif.