[Optimization of supercritical fluid extraction of ursolic acid from follum eriobotryae by response surface methodology].

OBJECTIVE: To optimize the conditions for extracting ursolic acid from Follum eriobotryae with supercritical fluid extration (SFE). METHODS: The contents of ursolic acid in the extracts were determined by HPLC. Based on single factor experiment and response surface methodology, a mathematical model for SFE of ursolic acid was built. RESULTS: The result showed that the optimum condition paremeters were as follows: temperature 61.6 degrees C, extraction pressure 25.8 MPa, dynamic extraction time 40 min. Under these conditions, theoretical extraction rate of ursolic acid was 3.96 mg/g. CONCLUSION: The eptimal process is reliable.