Anti-tumor therapy of glycyrrhetinic acid targeted liposome co-delivery of doxorubicin and berberine for hepatocellular carcinoma.

During the development of hepatocellular carcinoma (HCC), hepatic stellate cells undergo activation and transform into cancer-associated fibroblasts (CAFs) due to the influence of tumor cells. The interaction between CAFs and tumor cells can compromise the effectiveness of chemotherapy drugs and promote tumor proliferation, invasion, and metastasis. This study explores the potential of glycyrrhetinic acid (GA)-modified liposomes (lip-GA) as a strategy for co-delivery of berberine (Ber) and doxorubicin (Dox) to treat HCC. The characterizations of liposomes, including particle size, zeta potential, polydispersity index, stability and in vitro drug release, were investigated. The study evaluated the anti-proliferation and anti-migration effects of Dox&Ber@lip-GA on the Huh-7 + LX-2 cell model were through MTT and wound-healing assays. Additionally, the in vivo drug distribution and anti-tumor efficacy were investigated using the H22 + NIH-3T3-bearing mouse model. The results indicated that Dox&Ber@lip-GA exhibited a nanoscale particle size, accumulated specifically in the tumor region, and was efficiently taken up by tumor cells. Compared to other groups, Dox&Ber@lip-GA demonstrated higher cytotoxicity and lower migration rates. Additionally, it significantly reduced the deposition of extracellular matrix (ECM) and inhibited tumor angiogenesis, thereby suppressing tumor growth. In conclusion, Dox&Ber@lip-GA exhibited superior anti-tumor effects both in vitro and in vivo, highlighting its potential as an effective therapeutic strategy for combating HCC.

Effect of nano-silica coating combined with pressurized Ar treatment on postharvest quality and reactive oxygen species metabolism in sweet cherry fruit.

The mechanisms of effect of nano-silica coating and pressurized Ar on regulating reactive oxygen species (ROS) generation and scavenging in the senescence of sweet cherries remains unclear. The amounts of reactive oxygen species, hydrogen peroxide, non-enzymatic antioxidants and antioxidases, and cell membrane lipid peroxidation in sweet cherries were determined. Nano-silica coating, pressurized Ar, and the combination of these two treatments, all significantly delayed senescence by suppressing decay rate, and maintained good sensory quality. In additional, all treatments inhibited the generation and accumulation of superoxide anion and hydrogen peroxide, and mitigated the lipid peroxidation of cell membranes of sweet cherries. The combination of these two treatments maintained higher contents of ascorbic acid and glutathione, and enhanced the activities of antioxidases in sweet cherries. It is suggested that nano-silica coating and pressure Ar mediated the ROS metabolism, which might have a role in retaining the quality sweet cherries during cold storage.

[Optimization of supercritical fluid extraction of ursolic acid from follum eriobotryae by response surface methodology].

OBJECTIVE: To optimize the conditions for extracting ursolic acid from Follum eriobotryae with supercritical fluid extration (SFE). METHODS: The contents of ursolic acid in the extracts were determined by HPLC. Based on single factor experiment and response surface methodology, a mathematical model for SFE of ursolic acid was built. RESULTS: The result showed that the optimum condition paremeters were as follows: temperature 61.6 degrees C, extraction pressure 25.8 MPa, dynamic extraction time 40 min. Under these conditions, theoretical extraction rate of ursolic acid was 3.96 mg/g. CONCLUSION: The eptimal process is reliable.