RESUMO
Fibroblast-like synoviocytes (FLS) play a pivotal role in Rheumatoid arthritis (RA) pathogenesis through aggressive migration and invasion. Madecassoside (Madec), a triterpenoid saponin present in Centella asiatica herbs, has a potent anti-inflammatory effect. In the present study, Madec exerted an obvious therapeutic effect in reversing the histological lesions in adjuvant-induced arthritis (AIA) rats. To recognize the anti-rheumatoid potentials of Madec, we further investigated whether Madec interfered with FLS invasion and metalloproteinase (MMP) expression. In cultures of primary FLS isolated from the AIA rats, Madec (10 and 30 µmol·L-1) was proven to considerably inhibit migration and invasion of FLS induced by interleukin 1ß (IL-1ß), but exhibiting no obvious effect on cell proliferation. Madec repressed IL-1ß-triggered FLS invasion by prohibiting the expression of MMP-13. Additionally, Madec suppressed MMP-13 transcription via inhibiting the MMP-13 promoter-binding activity of NF-κB. Our results further showed that Madec down-regulated the translocation and phosphorylation of NF-κB as demonstrated by Western blotting and immunofluorescence assays. In conclusion, our results suggest that Madec exerts anti-RA activity via inhibiting the NF-κB/MMP-13 pathway.
Assuntos
Artrite Experimental/patologia , Regulação Enzimológica da Expressão Gênica/efeitos dos fármacos , Metaloproteinase 13 da Matriz/genética , NF-kappa B/metabolismo , Sinoviócitos/efeitos dos fármacos , Triterpenos/farmacologia , Animais , Antirreumáticos/química , Antirreumáticos/farmacologia , Antirreumáticos/uso terapêutico , Artrite Experimental/induzido quimicamente , Artrite Experimental/tratamento farmacológico , Movimento Celular/efeitos dos fármacos , Núcleo Celular/metabolismo , Células Cultivadas , Interleucina-1beta/farmacologia , NF-kappa B/genética , Fosforilação/efeitos dos fármacos , Transporte Proteico/efeitos dos fármacos , Ratos , Transdução de Sinais/efeitos dos fármacos , Sinoviócitos/metabolismo , Ativação Transcricional/efeitos dos fármacos , Triterpenos/química , Triterpenos/uso terapêuticoRESUMO
OBJECTIVE: To increase the recovery rate of ethyl acetate after extracting tripterygium wifordii extractum and to decrease product cost. METHOD: After extracting tripterygium wifordii extractum with ethyl acetate, 3 times saturated salt water was added in it so as to recovery ethyl acetate distilled under normal atmospheric pressure. Ethyl acetate containing salt water was purified through Na2SO4 column. RESULT: Ethyl acetate purified could be used repeatedly and the recovery rate was up to 85%. CONCLUSION: This method is completely adapted for mass production.