Effect of different operational modes on the performance of granular sludge in continuous-flow systems and the successions of microbial communities.

Continuous flow reactors with time intermittent operational (TIO) mode and spatial intermittent operational (SIO) mode were operated to evaluate the effects of operational modes on the removal performances, the characteristics of granules and the dynamics of microbial communities in simultaneous nitrification, denitrification and phosphorus removal (SNDPR) granular system. The results showed that the removal efficiency of TP, TN were 81.3%, 86.7% under TIO mode, and 70.6%, 77.4% under SIO mode, respectively. Meanwhile, the PN and value of PN/PS in SIO were higher than those in TIO. Besides, results of high-throughput pyrosequencing illustrated that the combination of filamentous archaea (Methanothrix) and filamentous bacteria (Thiothrix) had resulted in the increase of EPS and SVI under SIO mode. Finally, functional bacterial and archaeal species, involving HMA, AMA, AOA, DPAOs etc., were identified to reveal the effects of operational modes on the mechanism of nutrients removal in granular SNDPR continuous-flow system.

Effect of aeration modes on simultaneous nitrogen and phosphorus removal and microbial community in a continuous flow reactor with granules.

In this study, a continuous flow reactor with simultaneous nitrification, denitrification and phosphorus removal (SNDPR) granular sludge was operated in the continuous aeration (CA) and intermittent aeration (IA) modes to examine the effect of aeration on the performance of continuous-flow system. Then the experimental results showed that the IA1 mode (4 h aeration and 1 h non-aeration) could improve the simultaneous nitrogen and phosphorus removal and the settleability of granules in continuous flow system. Results of high-throughput pyrosequencing illustrated that the methanogens, AOA, ANAMMOX, DNB, denitrifying polyphosphate-accumulating organisms (DPAOs) were the important participant of simultaneous biological nutrients removal (SBNR), meanwhile, the IA1 mode could effectively inhibit the growth of filamentous microorganisms (Thiothrix and Acinetobacter). Finally, a conceptual model of the SNDPR granular microbial ecosystem under IA1 mode was proposed as a base for analyzing the mechanism of simultaneous nutrient removal in continuous flow system.

Inhibitory effects and molecular mechanisms of garlic organosulfur compounds on the production of inflammatory mediators.

SCOPE: Garlic is used for both culinary and medicinal purposes by many cultures. The garlic organosulfur compounds (GOSCs) are thought to be bioactive components. This study aims to clarify the antiinflammatory effects and molecular mechanisms of GOSCs in both cell and animal models. METHODS AND RESULTS: RAW264.7 cells were treated with six kinds of GOSCs to screen their influence on cyclooxygenase-2 and inducible nitric oxide synthase expression by Western blotting. Prostaglandin E2 and nitrite were measured by ELISA and Griess reaction, respectively. Cytokines in culture medium were assayed by the multiplex technology. Proteins were detected by Western blotting. Mouse paw edema was induced by LPS. The results revealed that diallyl trisulfide (DATS) was a strongest inhibitor for cyclooxygenase and inducible nitric oxide synthase among GOSCs, and reduced the levels of LPS-induced IL-6, IL-10, IL-12(p70), KC, MCP-1, and TNF-α. Cellular signaling analysis revealed that DATS downregulated AKT1/TGF-ß-activated kinase-mediated mitogen-activated protein kinase (MAPK) and nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB) pathways. Furthermore, DATS activated Nrf2-mediated expression of HO-1 and NQO1 and reduced LPS-induced intracellular reactive oxygen species, which may contribute to suppress inflammatory mediator production. Finally, in vivo data demonstrated that DATS attenuated LPS-induced mouse paw edema. CONCLUSION: DATS as a potential inhibitor revealed antiinflammatory effect in both cell and animal models by downregulating AKT1/TGF-ß-activated kinase-mediated NFκB and MAPK signaling pathways.