Analysis of oil synthesis pathway in Cyperus esculentus tubers and identification of oleosin and caleosin genes.

The tubers of the widely distributed Cyperus esculentus are rich in oil, and therefore, the plant is considered to have a high utilization value in the vegetable oil industry. Oleosins and caleosins are lipid-associated proteins found in oil bodies of seeds; however oleosins and caleosins genes have not been identified in C. esculentus. In this study, we performed transcriptome sequencing and lipid metabolome analysis of C. esculentus tubers at four developmental stages to obtain the information on their genetic profile, expression trends, and metabolites in oil accumulation pathways. Overall, 120,881 non-redundant unigenes and 255 lipids were detected; 18 genes belonged to the acetyl-CoA carboxylase (ACC), malonyl-CoA:ACP transacylase (MCAT), ß-ketoacyl-ACP synthase (KAS), and fatty acyl-ACP thioesterase (FAT) gene families involved in fatty acid biosynthesis, and 16 genes belonged to the glycerol-3-phosphate acyltransferase (GPAT), diacylglycerol acyltransferase 3 (DGAT3), phospholipid:diacylglycerol acyltransferase (PDAT), FAD2, and lysophosphatidic acid acyltransferase (LPAAT) gene families playing important roles in triacylglycerol synthesis. We also identified 9 oleosin- and 21 caleosin-encoding genes in C. esculentus tubers. These results provide detailed information on the C. esculentus transcriptional and metabolic profiles, which can be used as reference for the development of strategies to increase oil content in C. esculentus tubers.

Effect of micronutrients on algae in different regions of Taihu, a large, spatially diverse, hypereutrophic lake.

Eutrophication or excessive nutrient richness is an impairment of many freshwater ecosystems and a prominent cause of harmful algal blooms. It is generally accepted that nitrogen and phosphorus nutrients are the primary causative factor, however, for systems subject to large anthropogenic perturbation, this may no longer be true, and the role of micronutrients is often overlooked. Here we report a study on Lake Tai (Taihu), a large, spatially diverse and hypereutrophic lake in China. We performed small-scale mesocosm nutrient limitation bioassays using boron, iron, cobalt, copper, molybdenum, nitrogen and phosphorus on phytoplankton communities sampled from different locations in Taihu to test the relative effects of micronutrients on in situ algal assemblages. In addition to commonly-used methods of chemical and biological analysis (including algal phytoplankton counting), we used flow cytometry coupled with data-driven analysis to monitor changes to algal assemblages. We found statistically significant effects of limitation or co-limitation for boron, cobalt, copper and iron. For copper at one location chlorophyll-a was over four times higher for amendment with copper, nitrogen and phosphorous than for the latter two alone. Since copper is often proposed as amendment for the environmental management of harmful algal blooms, this result is significant. We have three primary conclusions: first, the strong effects for Cu that we report here are mutually consistent across chlorophyll-a results, count data, and results determined from a data-driven approach to flow cytometry. Given that we cannot rule out a role for a Fe-Cu homeostatic link in causing these effects, future research into MNs and how they interact with N, P, and other MNs should be pursued to explore new interventions for effective management of HABs. Second, in view of the stimulatory effect that Cu exhibited, management of HABs with Cu as an algal biocide may not always be advisable. Third, our approach to flow cytometry offers data confirming our results from chemical and biological analysis, however also holds promise for future development as a high-throughput tool for use in understanding changes in algal assemblages. The results from this study concur with a small and emerging body of literature suggesting that the potential role of micronutrients in eutrophication requires further consideration in environmental management.

Simultaneous Quantitative Determination of 12 Active Components in Yuanhu Zhitong Prescription by RP-HPLC Coupled with Photodiode Array Detection.

BACKGROUND: Yuanhu Zhitong prescription (YZP) is a famous traditional Chinese medicine formula, which is officially recorded in Chinese Pharmacopoeia for the treatment of stomach pain, hypochondriac pain, headache and dysmenorrhea caused by qi-stagnancy and blood stasis. It is the first report for the simultaneous determination of 12 active components in YZP. OBJECTIVE: A newly, simple, accurate and reliable method for the separation and determination of 12 active components (protopine, α-allocryptopine, coptisine, xanthotol, palmatine, dehydrocorydaline, glaucine, tetrahydropalmatine, tetrahydroberberine, imperatorin, corydaline, isoimperatorin) in YZP was developed and validated using HPLC-PAD. MATERIALS AND METHODS: The analytes were performed on a Phenomenex Luna-C18 (2) column (250×4.6 mm, 5.0 µm) with a gradient elution program using a mixture of acetonitrile and 0.1% phosphoric acid water solution (adjusted with triethylamine to pH 5.6) as mobile phase. Analytes were performed at 30°C with a flow rate of 1.0 mL/min. RESULTS: The validated method was applied to analyze four major dosage forms of YZP coming from different manufacturers with good linearity (r(2), 0.9981~0.9999), precision (RSD, 0.24~2.89%), repeatability (RSD, 0.15~3.34%), stability (RSD, 0.14~3.35%), recovery (91.13~110.81%) of the 12 components. CONCLUSION: The proposed method enables the separation and determination of 12 active components in a single run for the quality control of YZP.

Traditional Chinese medicine for the treatment of primary dysmenorrhea: how do Yuanhu painkillers effectively treat dysmenorrhea?

AIM: To examine the efficacy of YuanHu painkillers (YHP) as a treatment for primary dysmenorrhea and to reveal YHP's principle formula. METHODS: A Wistar rat uterine contraction model was utilized in this study. Rats were given 0.698g/kg YHP, 0.07g/kg tetrahydropalmatine (THP; YHP's main component), 0.02g/kg imperatorin (IMP), or THP+IMP (0.07+0.02g/kg) as polypharmacy (PG) by gavage. H&E staining and histopathological examination of the uteri tissue samples were performed. We then detected superoxide dismutase (SOD) and malondialdehyde (MDA), nitric oxide (NO), as well as inducible nitric oxide synthase (iNOS), i-κB, nuclear factor-κB (NF-κB), and cyclooxygenase-2 (COX-2) indices. RESULTS: PG significantly inhibited the uterine contraction of the primary dysmenorrhea rat model (p<0.05), and was significantly different than single-agent therapy (p<0.05). Histopathological examination showed inflammation in the uteri of the control group which YHP and its main constitutes alleviated. THP significantly inhibited the contraction of isolated uteri caused by Ach, PGF2α and oxytocin in a concentration-dependent fashion. THP and IMP both significantly affected the levels of NO, activation of NF-κB, up-regulated the expression of i-κB and down-regulated the expression of both iNOS and COX-2. IMP obviously decreased the level of MDA and increased the activation of SOD (p<0.05). PG obviously improved all the parameters mentioned above (p<0.05). CONCLUSIONS: YHP exerted protective effects on primary dysmenorrhea in rats and remarkably alleviated the severity of experimental primary dysmenorrhea. The combined strategy proved to be more effective than either THP or IMP alone and may have synergistic effects in combination in primary dysmenorrhea. Mechanisms that might account for the beneficial effects include abating oxidative stress, inhibiting over-inflammatory reaction, and alleviating the contraction of isolated rat uteri by inhibiting the influx of extracellular Ca(2+). Broad potential for future clinical practice is foreseeable.

[Study on the HPLC fingerprint of toad skin from different regions].

OBJECTIVE: To study the HPLC fingerprint of toad skin and provide a reliable method for quality control and identification. METHODS: It used HPLC for detection and computer aided similarity evaluation system for processing and analysing HPLC fingerprint. RESULTS: The common pattern of HPLC fingerprint of toad skin was astablished, 29 peaks were identified as the characteristic fingerprints, in which 9 peaks corresponded to 9 bufogenins. (2) Each samples' similarity of relative retention time was all above 0.99, but the similarity of relative peak areas was low. CONCLUSION: (1) The method is accurate and with good reproducibility. The fingerprints can be used for the identification and quality control of toad skin. (2) The toad skin from different regions are stable in composition, but the contents of the components are different.