RESUMO
This study aimed to investigate the effect of fatty acid-ethanol amine (FA-EA) derivatives (L1-L10) on the mitigation of intracellular lipid accumulation and downregulation of pro-inflammatory cytokines in vitro. First, the series of FA-EA derivatives were synthesized and characterized. Then, their cytotoxic, intracellular lipid accumulation and inhibition of pro-inflammatory cytokines were evaluated. The oil red O staining experiment showed that the tested compounds L4, L6, L8, L9, and L10 could reduce intracellular lipid accumulation induced by palmitic acid (PA). Moreover, ω-3/ω-6 PUFA-EA derivatives showed inhibitory effect on the production of pro-inflammatory cytokines in lipopolysaccharide (LPS) -stimulated RAW 264.7 cells. ω-3/ω-6 PUFA-EA derivatives at a concentrations of 10 µM could significantly decrease mRNA levels of IL-6, IL-1ß, and TNF-α, inhibit NO production, and alleviate the protein expression of IL-1ß in lipopolysaccharide (LPS)-stimulated RAW 264.7 cells. These data suggest that ω-3 PUFA-EA derivatives can be beneficial for further pharmaceutical development to treat chronic low-grade inflammation diseases such as obesity.
Assuntos
Ácidos Graxos Ômega-3 , Lipopolissacarídeos , Humanos , Lipopolissacarídeos/farmacologia , Ácidos Graxos Ômega-3/farmacologia , Citocinas/genética , Citocinas/metabolismo , Inflamação/induzido quimicamente , Inflamação/tratamento farmacológico , Ácidos GraxosRESUMO
As high-efficiency, safe, and low-drug resistant antibacterial agents, silver nanoparticles (AgNPs) have been widely applied in food and biomedicine. AgNPs was prepared using mango peel extract (MPE) as green and cheap reducing agent and stabilizer. In addition, a novel of preservative film material was developed with polylactic acid (PLA) as protective and substrate. AgNPs was characterized by XPS, XRD and TEM, and the size of AgNPs were in the range of 2.5-6.5 nm. The addition of AgNPs improved the mechanical properties of the film and its barrier ability to water vapor and oxygen. The film exhibited excellent antibacterial properties, and the inhibition rate against Escherichia coli and Staphylococcus aureus were above 95%. Furthermore, in terms of safety, the silver migration and cytotoxicity of the film met the relevant standards, and the shelf life of strawberries was significantly extended.
Assuntos
Embalagem de Alimentos , Mangifera/química , Nanopartículas Metálicas/química , Extratos Vegetais/química , Poliésteres/química , Prata/química , Animais , Compostos de Bifenilo/química , Linhagem Celular , Sobrevivência Celular/efeitos dos fármacos , Óxidos N-Cíclicos/química , Escherichia coli/efeitos dos fármacos , Fragaria , Humanos , Imidazóis/química , Nanopartículas Metálicas/ultraestrutura , Testes de Sensibilidade Microbiana , Tamanho da Partícula , Espectroscopia Fotoeletrônica , Picratos/química , Prata/farmacologia , Espectroscopia de Infravermelho com Transformada de Fourier , Staphylococcus aureus/efeitos dos fármacos , Termogravimetria , Água/química , Difração de Raios XRESUMO
This paper reports a strategy for preparing polyether polyols from corn starch, with (i) a mixture of polyethylene glycol 400 and glycerin (7:3, w/w) as the liquefying solvent and (ii) a spent-coffee-derived solid-acid catalyst (SC-SAC) (1:10, w/w, SC-SAC/starch) at 433 K for 1.5 h, under which conditions the liquefaction yield exceeded 99 %. The SC-SAC was prepared via hydrothermal carbonization at 453 K for 12 h, followed by sulfonation with H2SO4 at 343 K for 10 h. The liquefied starch product (SLP) was then used to plasticize poly(vinyl alcohol) (PVA) films with various mixing ratios. The optimal 0.4 SLP/PVA blend film exhibited good mechanical properties (tensile strength 38.07 MPa, elongation at break 1199 %), good transparency, and excellent flexibility. The results highlight the possibility of using SLP/PVA films in the development of degradable packaging materials.