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1.
Bioresour Technol ; 383: 129227, 2023 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-37244313

RESUMO

To achieve advanced nitrogen removal from low-carbon wastewater, a partial-nitrification/anammox and endogenous partial-denitrification/ anammox (PN/A-EPD/A) process was developed in a sequential batch biofilm reactor (SBBR). Advanced nitrogen was achieved with the effluent total nitrogen (TN) of 3.29 mg/L when the influent COD/TN and the TN were 2.86 and 59.59 mg/L, respectively. This was attributed to a stable PN/A-EPD/A, which was achieved through the integration of four strategies, including treating the inoculated sludge with free nitrous acid, inoculating anammox biofilm, discharging excess activated sludge and residual ammonium at the end of oxic stage. The 16S rRNA high-throughput sequencing results demonstrated that anammox bacteria coexisted with ammonia oxidizing bacteria, nitrite oxidizing bacteria, denitrifying glycogen accumulating organisms (DGAOs) and denitrifying phosphorus accumulating organisms (DPAOs) in biofilms. The abundance of anammox bacteria in the inner layer of the biofilm is higher, while that of DGAOs and DPAOs is higher in the outer layer.


Assuntos
Nitrificação , Águas Residuárias , Esgotos/microbiologia , Desnitrificação , Nitrogênio , Carbono , RNA Ribossômico 16S/genética , Oxidação Anaeróbia da Amônia , Reatores Biológicos/microbiologia , Bactérias/genética , Fósforo , Oxirredução
2.
Biomed Pharmacother ; 150: 112973, 2022 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-35468581

RESUMO

Dioscin (Dio), steroid saponin, exists in several medicinal herbs with potent anticancer efficacy. This study aimed to explore the effect of Dio on the immune-related modulation and synergistic therapeutic effects of the herpes simplex virus thymidine kinase/ganciclovir (HSV-Tk/GCV) suicide gene therapy system in murine melanoma, thereby providing a research basis to improve the potential immunomodulatory mechanism underlying combination therapy. Using both in vitro and in vivo experiments, we confirmed the immunocidal effect of Dio-potentiated suicide gene therapy on melanoma. The results showed that Dio upregulated connexin 43 (Cx43) expression and improved gap junction intercellular communication (GJIC) in B16 cells while increasing the cross-presentation of antigens by dendritic cells (DCs), eventually promoting the activation and antitumor immune killing effects of CD8+ T lymphocytes. In contrast, inhibition or blockade of the GJIC function (overexpression of mutant Cx43 tumor cells/Gap26) partially reversed the potentiating effect. The significant synergistic effect of Dio on HSV-Tk/GCV suicide gene therapy was further investigated in a B16 xenograft mouse model. The increased number and activation ratio of CD8+ T lymphocytes and the levels of Gzms-B, IFN-γ, and TNF-α in mice reconfirmed the potential modulatory effects of Dio on the immune system. Taken together, Dio targets Cx43 to enhance GJIC function, improve the antigens cross-presentation of DCs, and activate the antitumor immune effect of CD8+ T lymphocytes, thereby providing insights into the potential immunomodulatory mechanism underlying combination therapy.


Assuntos
Conexina 43 , Melanoma , Animais , Comunicação Celular , Conexina 43/genética , Conexina 43/metabolismo , Apresentação Cruzada , Diosgenina/análogos & derivados , Ganciclovir/farmacologia , Ganciclovir/uso terapêutico , Junções Comunicantes/metabolismo , Terapia Genética/métodos , Humanos , Melanoma/tratamento farmacológico , Melanoma/terapia , Camundongos , Simplexvirus/genética , Simplexvirus/metabolismo , Timidina Quinase/genética , Timidina Quinase/metabolismo , Timidina Quinase/farmacologia
3.
Biomed Pharmacother ; 118: 109198, 2019 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-31336342

RESUMO

ω-3 fish oil fat emulsions contain a considerable quantity of unsaturated carbon-carbon double bonds, which undergo lipid peroxidation to yield low-dose aldehydes. These aldehydes may stimulate the production of antioxidant enzymes, thereby mitigating myocardial oxidative damage. This study aims to (1) verify the cardioprotective effect of ω-3 fish oil fat emulsion in vivo and in vitro, and (2) determine whether aldehyde stress is a protective mechanism. For modeling purposes, we pretreated rats with 2 ml/kg of a 10% ω-3 fish oil fat emulsion for 5 days in order to generate a sufficient aldehyde stress response to trigger the production of antioxidant enzymes, and we obtained similar response with H9C2 cells that were pretreated with a 0.5% ω-3 fish oil fat emulsion for 24 h. ω-3 fish oil fat emulsion pretreatment in vivo reduced the myocardial infarct size, decreased the incidence of arrhythmias, and promoted the recovery of cardiac function after myocardial ischemia/reperfusion injury. Once the expression of nuclear factor E2-related factor 2 (Nrf2) was silenced in H9C2 cells, aldehydes no longer produced enough antioxidant enzymes to reverse the oxidative damage caused by tert-butyl hydroperoxide (TBHP). Our results demonstrated that ω-3 fish oil fat emulsion enhanced the inhibition of oxidation and production of free radicals, and alleviated myocardial oxidative injury via activation of the Nrf2 signaling pathway.


Assuntos
Aldeídos , Ácidos Graxos Ômega-3 , Óleos de Peixe , Peroxidação de Lipídeos , Infarto do Miocárdio , Traumatismo por Reperfusão Miocárdica , Animais , Masculino , Aldeídos/metabolismo , Antioxidantes/metabolismo , Linhagem Celular , Ácidos Graxos Ômega-3/administração & dosagem , Ácidos Graxos Ômega-3/farmacologia , Óleos de Peixe/administração & dosagem , Óleos de Peixe/farmacologia , Peroxidação de Lipídeos/efeitos dos fármacos , Mioblastos Cardíacos/efeitos dos fármacos , Mioblastos Cardíacos/metabolismo , Infarto do Miocárdio/metabolismo , Infarto do Miocárdio/prevenção & controle , Traumatismo por Reperfusão Miocárdica/metabolismo , Traumatismo por Reperfusão Miocárdica/prevenção & controle , Ratos Sprague-Dawley , Fator 2 Relacionado a NF-E2/metabolismo
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