RESUMO
Huang-Qin Decoction (HQD) is a classic prescription for diarrhea in Chinese medicine treatment. Recent studies have demonstrated that HQD and its modified formulation PHY906 could ameliorate irinotecan (CPT-11) induced gastrointestinal (GI) toxicity and enhance its anticancer therapeutic efficacy. Nevertheless, which constituents in HQD are effective is still unclear so far. The study aims to screen out the key bioactive components combination from HQD that could enhance the anticancer effect of CPT-11. First, the potential bioactive constituents were obtained through system pharmacology strategy. Then the bioactivity of each constituent was investigated synthetically from the aspects of NCM460 cell migration, TNF-α release of THP-1-derived macrophage and MTT assay in HCT116 cell. The contribution of each constituent in HQD was evaluated using the bioactive index Ei, which taken the content and bioactivity into comprehensive consideration. And then, the most contributing constituents were selected out to form a key-component combination. At last, the bioefficacy of the key-component combination was validated in vitro and in vivo. As a result, a key-component combination (HB4) consisting of four compounds baicalin, baicalein, glycyrrhizic acid and wogonin was screened out. In vitro assessment indicated that HB4 could enhance the effect of CPT-11 on inhibiting cell proliferation and inducing apoptosis in HCT116. Furthermore, the in vivo study confirmed that HB4 and HQD have similar pharmacological activity and could both enhance the antitumor effect of CPT-11 in HCT116 xenograft model. Meanwhile, HB4 could also reduce the CPT-11 induced GI toxicity.
Assuntos
Antineoplásicos/farmacologia , Medicamentos de Ervas Chinesas , Irinotecano/farmacologia , Scutellaria baicalensis , Animais , Apoptose , Proliferação de Células , Modelos Animais de Doenças , Medicamentos de Ervas Chinesas/farmacologia , Células HCT116 , Humanos , Scutellaria baicalensis/química , Ensaios Antitumorais Modelo de XenoenxertoRESUMO
The ripened seeds of Strychnos nux-vomica L. have been extensively used as herbal medicines in Asian countries. Dihydroindole-type alkaloids are not only the active constituents but also the toxicants in Strychnos. However, the simultaneous determination of these alkaloids in both crude and processed Semen Strychni is still lacking. The present study represents the first quantitation and relative quantitation assay of 12 dihydroindole-type alkaloids in Strychnos nux-vomica unprocessed and sand-processed seeds using high-performance liquid chromatography coupled with diode array detection and mass spectrometry. The relative concentration of ten alkaloids was calculated by semi-quantification using the internal standard and their amounts in unprocessed and detoxified Semen Strychni were compared. We report here for the first time the significant increase of the two alkaloids, 19-N-methyl-strychnine, and 2,3-dimethoxy-19-N-methyl-strychnine, during the processing of Semen Strychni. Our study provides new insight into the true complexity of seed processing procedure and valuable information for assessing the efficacy and safety for clinical applications of Semen Strychni-containing drugs.
Assuntos
Alcaloides Indólicos/análise , Sementes/química , Strychnos nux-vomica/química , Cromatografia Líquida de Alta Pressão , Espectrometria de Massas , Estrutura MolecularRESUMO
Triptolide (TP), a major active ingredient of Tripterygium wilfordii, exerts potent immunosuppressive effects in the treatment of rheumatoid arthritis but is not widely used in clinical practice due to its multiorgan toxicity, particularly hepatotoxicity, nephrotoxicity, and reproductive toxicity. An LC-MS/MS approach was employed to explore the endocrine-disrupting effects of TP. The endocrine-disrupting effects of various concentrations (0-100 nM) of TP for 48 hour were firstly investigated using an in vitro model (H295R cell line). It was found that TP did not decrease cell viability. The transcriptional levels of steroidogenic enzymes in H295R cells were assessed by quantificational real-time polymerase chain reaction. The possible adrenal and endocrine effects of oral administration of TP (0, 50, and 500 µg/kg) for 28 days on both normal and collagen-induced arthritis (CIA) rats were also explored. The serum and adrenal tissue hormone levels (corticosterone and progesterone) and adrenal histopathology were analyzed, with the results that TP significantly decreased the level of cortisol in H295R cells and the level of plasma corticosterone in both normal and CIA rats. Histological alterations in adrenal cortex were observed at the dose of 500 µg/kg. Exposure to TP for 48 hour had an obvious inhibitory effect on the messenger RNA transcript levels of HSD3B2, CYP21A2, CYP17A1, and CYP11B1, which is essential for the synthesis of corticosteroids. In a word, TP leads to the disorder of corticosteroid synthesis and secretion, and corticosteroid may be a potential biomarker for the treatment of multiorgan toxicity of TP.
Assuntos
Corticosteroides/metabolismo , Diterpenos/toxicidade , Hormônios Gonadais/metabolismo , Fenantrenos/toxicidade , Extratos Vegetais/toxicidade , Córtex Suprarrenal/patologia , Animais , Linhagem Celular Tumoral , Sobrevivência Celular/efeitos dos fármacos , Cromatografia Líquida , Compostos de Epóxi/toxicidade , Feminino , Expressão Gênica/efeitos dos fármacos , Humanos , Progesterona Redutase/metabolismo , RNA Mensageiro/efeitos dos fármacos , RNA Mensageiro/genética , Ratos , Ratos Wistar , Transdução de Sinais/efeitos dos fármacos , Esteroide Hidroxilases/metabolismo , Espectrometria de Massas em Tandem , Tripterygium/químicaRESUMO
The quality control research of traditional Chinese medicine (TCM) is lagged far behind the space of progress in modernization and globalization. Thus the concept of quality marker (Q-marker) was proposed recently to guide the quality investigations of TCM. However, how to discover and validate the Q-marker is still a challenge. In this paper, a system pharmacology based strategy was proposed to discover Q-marker of HuangQin decoction (HQD) to attenuate Intestinal Damage. Using this strategy, nine measurable compounds including paeoniflorin, baicalin, scutellarein, liquiritigenin, norwogonin, baicalein, glycyrrhizic acid, wogonin, and oroxylin A were screened out as potential markers. Standard references of these nine compounds were pooled together as components combination according to their corresponding concentration in HQD. The bioactive equivalence between components combination and HQD was validated using wound healing test and inflammatory factor determination experiment. The comprehensive results indicated that components combination is almost bioactive equivalent to HQD and could serve as the Q-markers. In conclusion, our study put forward a promising strategy for Q-markers discovery.
RESUMO
Realgar nanoparticles (NPs) are increasingly used as therapeutic agents for their enhanced anti-proliferation effect and cytotoxicity on cancer cells. However, the alteration of particle size may enhance biological reactivity as well as toxicity. A LC/MS and GC/MS based metabolomics approach was employed to explore the mechanism of realgar NPs-induced hepatotoxicity and identify potential biomarkers. Male Sprague-Dawley rats were administrated intragastrically with realgar or realgar NPs at a dose of 1.0 g·kg-1·d-1 for 28 days and toxic effects of realgar NPs on liver tissues were examined by biochemical indicator analysis and histopathologic examination. Increased levels of serum enzymes and high hepatic steatosis were discovered in the realgar NPs treated group. Multivariate data analysis revealed that rats with realgar NPs-induced hepatotoxicity could be distinctively differentiated from the animals in the control and realgar treated groups. In addition, 21 and 32 endogenous metabolites were apparently changed in the serum and live extracts, respectively. Realgar NPs might induce free fatty acid and triglyceride accumulation, resulting in hepatotoxicity. In conclusion, the present study represents the first comprehensive LC/MS- and GC/MS-based metabolomics analysis of realgar NPs-induced hepatotoxicity, which may help further research of nanotoxicity.
Assuntos
Cromatografia Líquida/métodos , Cromatografia Gasosa-Espectrometria de Massas/métodos , Fígado/efeitos dos fármacos , Espectrometria de Massas/métodos , Metabolômica/métodos , Nanopartículas/toxicidade , Animais , Biomarcadores/sangue , Biomarcadores/química , Ácidos Graxos/metabolismo , Fígado/química , Fígado/metabolismo , Masculino , Ratos , Ratos Sprague-Dawley , Triglicerídeos/metabolismoRESUMO
Phyllanthus Urinaria L. (PUL) is a traditional Chinese medicine used to treat hepatic and renal disorders. However, the mechanism of its hepatoprotective action is not fully understood. In the present study, blood biochemical indexes and liver histopathological changes were used to estimate the extent of hepatic injury. GC/MS and LC/MS-based untargeted metabolomics were used in combination to characterize the potential biomarkers associated with the protective activity of PUL against CCl4-induced liver injury in rats. PUL treatment could reverse the increase in ALT, AST and ALP induced by CCl4 and attenuate the pathological changes in rat liver. Significant changes in liver metabolic profiling were observed in PUL-treated group compared with liver injury model group. Seventeen biomarkers related to the hepatoprotective effects of PUL against CCl4-induced liver injury were screened out using nonparametric test and Pearson's correlation analysis (OPLS-DA). The results suggested that the potential hepatoprotective effects of PUL in attenuating CCl4-induced hepatotoxicity could be partially attributed to regulating L-carnitine, taurocholic acid, and amino acids metabolism, which may become promising targets for treatment of liver toxicity. In conclusion, this study provides new insights into the mechanism of the hepatoprotection of Phyllanthus Urinaria.
Assuntos
Doença Hepática Induzida por Substâncias e Drogas/prevenção & controle , Medicamentos de Ervas Chinesas/administração & dosagem , Fígado/metabolismo , Phyllanthus/química , Aminoácidos/metabolismo , Animais , Tetracloreto de Carbono/efeitos adversos , Doença Hepática Induzida por Substâncias e Drogas/metabolismo , Cromatografia Gasosa-Espectrometria de Massas , Humanos , Fígado/efeitos dos fármacos , Fígado/enzimologia , Masculino , Metabolômica , Ratos , Ratos Sprague-Dawley , Ácido Taurocólico/metabolismoRESUMO
Huangqin decoction (HQD), a traditional Chinese medicine (TCM), has been widely used to treat gastrointestinal syndrome in China for thousands of years. Chemotherapy drug irinotecan (CPT-11) is used clinically to treat various kinds of cancers but limited by its side effects, especially delayed diarrhea. Nowadays, HQD has been proved to be effective in attenuating the intestinal toxicity induced by CPT-11. HQD consists of four medicinal herbs including Scutellaria baicalensis Georgi, Glycyrrhiza uralensis Fisch, Paeonia lactiflora Pall, and Ziziphus jujuba Mill. Due to its complexity, the role of each herb and the multi-herb synergistic effects of the formula are poorly understood. In order to quantitatively assess the compatibility effects of HQD, mass spectrometry-based untargeted metabolomics studies were performed. The serum metabolic profiles of rats administered with HQD, single S. baicalensis decoction, S. baicalensis-free decoction and baicalin/baicalein combination were compared. A time-dependent trajectory upon principal component analysis was firstly used to visualize the overall differences. Then metabolites deregulation score and relative area under the curve were calculated and used as parameters to quantitatively evaluate the compatibility effects of HQD from the aspect of global metabolic profile and the specifically altered metabolites, respectively. The collective results indicated that S. baicalensis played a crucial role in the therapeutic effect of HQD on irinotecan-induced diarrhea. Both HQD and SS decoction regulated glycine, serine and threonine pathway. This study demonstrated that metabolomics was a promising tool to elucidate the compatibility effects of TCM or combinatorial drugs.
RESUMO
With a great difference in therapeutic effects of Mahuang (MH, the stems of Ephedra sinica) and Mahuanggen (MHG, the roots of Ephedra sinica), chemical differences between MH and MHG should be investigated. In the present study, gas chromatography-mass spectrometry (GC-MS)-based plant metabolomics was employed to compare volatile oil profiles of MH and MHG. The antioxidant activities of volatile oils from MH and MHG were also compared. 32 differential chemical markers were identified according to the variable importance in the projection (VIP) value of orthogonal partial least squares discriminant analysis (OPLS-DA) and P value of Mann-Whitney test. Among them, chemical markers of tetramethylpyrazine (TMP) and α-terpineol were quantified. Their contents were much higher in most MH samples compared with MHG. The antioxidant assay demonstrated that MH had significantly higher free radical-scavenging activity than MHG. Although MH and MHG derived from the same medicinal plant, there was much difference in their volatile oil profiles. MH samples had significantly higher content of two reported pharmacologically important chemical markers of TMP and α-terpineol, which may account for their different antioxidant activities.
Assuntos
Medicamentos de Ervas Chinesas/química , Ephedra sinica/química , Óleos Voláteis/química , Raízes de Plantas/química , Caules de Planta/química , Cromatografia Gasosa-Espectrometria de Massas , MetabolômicaRESUMO
AIM: The aim of this work was to establish a specific and sensitive method to comprehensively investigate and compare chemical constituents of Fuzi-Gancao herb pair (FG), consisting of Aconitum carmichaelii Debeaux (Fuzi, Chinese) and Roast Radix Glycyrrhizae (Glycyrrhiza glabra L., Gancao, in Chinese) and Fuzi alone to explore the underlying interaction mechanism of FG. METHOD: An ultra-fast liquid chromatography-ion trap/time-of-flight mass spectrometry (UFLC/MS-IT-TOF) method using diazepam as internal standard was developed for the identification and semi-quantitative analysis of the phytochemical constituents of Fuzi and FG. Chromatographic separation was achieved on a UFLC column using a gradient program with 40 mmol·L(-1) ammonium acetate and acetonitrile as the mobile phase. RESULTS: Fifty-one of the sixty compounds, including forty-five C19-diterpenoid alkaloids and six C20-diterpenoid alkaloids were tentatively identified in the extracts of Fuzi and FG through accurate mass measurements and fragmentation patterns. Comparing the contents of these alkaloids in these two extracts, it was found that the diester-diterpenoid alkaloids (DDAs) and the alkylolamine-diterpenoid alkaloids (ADAs) were increased, while the monoester-diterpenoid alkaloids (MDAs) were decreased in the extracts of FG. CONCLUSION: This work provided comprehensive information for the quality control of Fuzi preparations, and the further investigation on the compatibility mechanisms of FG.
Assuntos
Aconitum/química , Cromatografia Líquida de Alta Pressão/métodos , Medicamentos de Ervas Chinesas/química , Glycyrrhiza/química , Espectrometria de Massas/métodos , Interações Medicamentosas , Estrutura MolecularRESUMO
INTRODUCTION: For centuries, Semen Strychni (the ripened seeds of Strychnos nux-vomica) has been used extensively as a herbal medicine in Asian countries. However, the chemical composition of the dihydroindole-type alkaloids contained in processed Semen Strychni is not fully understood. OBJECTIVE: To develop an improved strategy using mass defect filtering (MDF) in combination with MS(n) analysis and theoretical calculations for identification and structural characterisation of dihydroindole-type alkaloids in processed Semen Strychni extracts. METHODS: The experimental work was conducted using a high-performance liquid chromatography coupled with electrospray ionisation ion trap time-of-flight mass spectrometry (HPLC-ESI/IT-TOF/MS) system. Upon acquisition of full-scan MS data, the potential dihydroindole-type alkaloids were screened using a well-defined mass defect range of 50 mDa. With the assistance of MS(n) analysis, the diagnostic fragment ions (DFIs) were used as primary screening references for targeting the characteristic analogues. For better discrimination of the isomers, quantum chemical calculations were utilised to provide additional structural information. RESULTS: Twenty-four dihydroindole-type alkaloids, including four that were previously not described, were tentatively identified. CONCLUSION: A new, rapid and sensitive method was developed for the discovery and characterisation of dihydroindole-type alkaloids in extracts of processed Semen Strychni. The successful application of this method indicates a potential for adaptation to other classes of natural product from other sources.
Assuntos
Alcaloides/análise , Alcaloides/química , Cromatografia Líquida de Alta Pressão/métodos , Espectrometria de Massas por Ionização por Electrospray/métodos , Strychnos nux-vomica/química , Isomerismo , Plantas Medicinais/química , Espectrometria de Massas em Tandem/métodosRESUMO
Saikosaponins (SSs) are a class of triterpene saponins with a wide spectrum of bioactivities. A sensitive liquid chromatography tandem mass spectrometry (LC-MS/MS) method was developed for simultaneous determination of saikosaponin a, saikosaponin c, saikosaponin d and saikosaponin b2 in rat plasma. Plasma samples were prepared by liquid-liquid extraction. The analytes and the internal standard (IS) digoxin were well separated on an octadecyl column using gradient elution and analyzed by monitoring the fragmentation transition pair of anionic adducts to deprotonated molecules in negative-mode electrospray. By neutral loss of HCOOH, the transition pairs of m/z 825 â 779 for SSa, SSd, SSb2 and the IS, and m/z 971 â 925 for SSc were sensitive for MS/MS detection with the lower limits of quantification in the range of 0.20-0.40 ng/mL. Method validation experiments were performed, including selectivity, precision, accuracy, linearity, matrix effect, recovery and stability. The validated method was further applied to determine the pharmacokinetics parameters of SSa, c and d in rats following a single oral administration of the extract of chaihu (the dried roots of Bupleurum chinense DC).
Assuntos
Cromatografia Líquida/métodos , Ácido Oleanólico/análogos & derivados , Saponinas/sangue , Espectrometria de Massas em Tandem/métodos , Animais , Ânions/química , Digoxina/sangue , Estabilidade de Medicamentos , Medicamentos de Ervas Chinesas , Modelos Lineares , Extração Líquido-Líquido , Masculino , Ácido Oleanólico/sangue , Ácido Oleanólico/química , Ácido Oleanólico/farmacocinética , Ratos , Ratos Sprague-Dawley , Reprodutibilidade dos Testes , Saponinas/química , Saponinas/farmacocinética , Sensibilidade e EspecificidadeRESUMO
Three new alkaloids, 15b-dehydro-5- N-acetylardeemin ( 3), 10-phenyl-[12]-cytochalasins Z16 ( 6) and Z17 ( 7), were characterized from the liquid culture of the endophytic fungus ASPERGILLUS TERREUS IFB-E030 along with six known derivatives, 5- N-acetylardeemin ( 1), 15b- ß-hydroxyl-5- N-acetylardeemin ( 2), cytochalasin E ( 4), rosellichalasin ( 5), cytochalasins Z11 ( 8), and Z13 ( 9). The structures of the new metabolites were established mainly by a combination of their 1D- and 2D-NMR spectra, single crystal X-ray diffraction, and the modified Mosher reaction. Biological assays indicated that cytochalasin Z17 ( 7) had moderate cytotoxicity against human nasopharyngeal epidermoid tumor KB cell line with an IC (50) value of 26.2 µM.
Assuntos
Artemisia annua/microbiologia , Aspergillus/química , Citocalasinas/farmacologia , Citotoxinas/farmacologia , Alcaloides Indólicos/farmacologia , Fracionamento Químico , Citocalasinas/química , Citocalasinas/isolamento & purificação , Citotoxinas/química , Citotoxinas/isolamento & purificação , Humanos , Alcaloides Indólicos/química , Alcaloides Indólicos/isolamento & purificação , Células KB , Ressonância Magnética Nuclear Biomolecular , Pirimidinonas/química , Pirimidinonas/isolamento & purificação , Pirimidinonas/farmacologia , Difração de Raios XRESUMO
The aim is to develop a simple and specific method for the extraction and chemical fingerprinting of Andrographis paniculata (Burm. f.) Nees and to apply the method to this drug from different regions. High-performance liquid chromatographic (HPLC) with gradient elution is used for developing the fingerprints, and liquid chromatography electrospray ionization mass spectrometry (LC-ESI-MS) technique is employed to identify the component of the fingerprints. Nine peaks are selected as common peaks, and six compounds are elucidated by MS data. Twenty-three samples of A. paniculata from different regions of China are collected and detected by HPLC fingerprinting. Comparisons of the chromatograms show that there are obvious differences in the content of each component contained between the habitat samples in China. The results of hierarchical cluster analysis show that these samples can be clustered reasonably into three groups, and the growth of A. paniculata and their internal quality are related to their habitat. The HPLC fingerprint developed allows simple identification of A. paniculata from many natural drugs.
Assuntos
Andrographis/química , Cromatografia Líquida de Alta Pressão/métodos , Extratos Vegetais/química , China , Espectrometria de Massas por Ionização por Electrospray/métodosRESUMO
A new 1,4-phenanthraquinone was isolated from the roots of Menispermum dauricum. The structure was elucidated as 7-hydroxy-3,6-dimethoxy-1,4-phenanthraquinone on the basis of spectral evidence.
Assuntos
Menispermum , Fenantrenos/química , Fitoterapia , Extratos Vegetais/química , Quinonas/química , Humanos , Espectroscopia de Ressonância Magnética , Raízes de PlantasRESUMO
OBJECTIVE: To determine Verbascoside in Galeobdolon chinense. METHOD: HPLC method was used, with the column packed with Kromasil C18(4.6 mm x 150 mm, 5 microns). The mobile phase was a mixture of acetonitrile and 1% acetic acid in a ratio of 13:87, and the ultraviolet wavelength was set at 350 nm. RESULT: The average recovery was 97.0%, the RSD being 1.69%. CONCLUSION: This method is sensitive and reliable. It can be used to determine being Verbascoside in Galeobdolon chinense.