Pilot study of toxicological safety evaluation in acute and 28-day studies of selenium nanoparticles decorated by polysaccharides from Sargassum fusiforme in Kunming mice.

The monodisperse and nearly spherical selenium nanoparticles decorated by polysaccharides from Sargassum fusiforme (SFPS-SeNPs) were prepared, characterized, and evaluated in acute and 28-day toxicological safety studies. In the acute toxicity study, mice underwent oral administration of 26.94, 40.28, 60.21, 90.11, and 134.70 mg Se/kg of SFPS-SeNPs for 14 days. In the 28-day study, mice underwent a daily oral administration of 17.75, 8.87, and 4.43 mg Se/kg/day of SFPS-SeNPs, 4.43 mg Se/kg/day of Na2 SeO3 , and normal saline for 28 days. The animals' general behavior, body weight, biochemical and hematologic parameters, organ coefficients, pathological morphology, Se content, and accumulation rate of Se in vital organs were determined. Results showed that the median lethal dose was 88.76 Se mg/kg and no observed adverse effect level was 4.43 mg Se/kg/day for 28 days. Compared with Na2 SeO3 , SFPS-SeNPs may lead to slightly higher toxicological effects, and it probably accumulates in the liver in the oral dose of 4.43 mg Se/kg/day in Kunming mice. SFPS and nanotechnology can reduce the toxicity of selenium, and SFPS-SeNPs or SeNPs-polysaccharides can be potential candidates for drug delivery and food supplement. PRACTICAL APPLICATION: Selenium nanoparticles decorated by polysaccharides from Sargassum fusiforme can improve the stability and reduce the toxicity of selenium nanoparticles. These results of the toxicological safety evaluation can lay the foundation for the safe utilization of selenium nanoparticles decorated by polysaccharides and expand their application in the field of food and medicine.

Effect of Alteplase Thrombolysis on Coagulation Function and Nerve Function of Patients with Ischemic Stroke.

Objective: To investigate the effects of alteplase thrombolysis on coagulation function and nerve function of patients with ischemic stroke. Methods: 76 cases with ischemic stroke receiving thrombolytic therapy in Cangzhou Central Hospital from November 2018 to November 2019 were recruited. They were assigned via the random number table method at a ratio of 1 : 1 to receive alteplase thrombolysis either within 3h after the onset (observation group) or within 3-4.5 h after the onset (control group), followed by aspirin administration after no bleeding in cranial computed tomography (CT). Outcome measures included plasma fibrinogen (FIB), activated partial prothrombin time (APTT), platelet (PLT) levels, the National Institute of Health stroke scale (NIHSS) score, and adverse events. Results: Alteplase thrombolysis within 3 h was associated with better prothrombin time (PT), APTT, FIB, and PLT levels versus thrombolysis within 3-4.5 h (P < 0.05). Thrombolysis within 3 h showed significantly lower NIHSS scores versus within 3-4.5 h (P < 0.05). The two groups showed a similar incidence of adverse events (X 2 = 2.963, P=0.615). Conclusion: Alteplase thrombolysis showed benefits in mitigating the coagulation function and nerve function damage of patients with ischemic stroke, especially within 3 hours after the onset, with a high safety profile.

Comparison of different probiotics in the treatment of acute diarrhea in children: A protocol for systematic review and network meta-analysis.

BACKGROUND: Acute diarrhea has a serious impact on the health and lives of children. Previous studies have shown that probiotics have positive and reliable efficacy in the treatment of acute diarrhea in children, but the efficacy of different types of probiotics varies. This study will evaluate the clinical efficacy of different kinds of probiotics in the treatment of acute diarrhea in children by means of network meta-analysis. METHODS: According to the retrieval strategy, randomized controlled studies on probiotics in the treatment of acute diarrhea in children will be searched from PubMed, Embase, Web of Science, the Cochrane Library, CNQI, Wanfang, VIP, and Chinese biomedical databases. The retrieval time limit will be from the establishment of the database to January 2022. The quality level of the included studies will be assessed using the Cochrane Risk Bias Assessment Tool and the strength of evidence for outcome measures will be assessed using the Grading of Recommendation Assessment, Development, and Evaluation method. All data analysis will be performed by Revman5.3, Gemtc 0.14.3 and Stata 14.0. RESULTS: This study will evaluate the efficacy of different kinds of probiotics in the treatment of acute diarrhea in children by evaluating diarrhea duration, stool frequency, length of hospital stay, adverse reactions, etc. CONCLUSIONS: This study will provide a reliable evidence-based basis for the selection of probiotics for the treatment of acute diarrhea in children. ETHICS AND DISSEMINATION: Private information from individuals will not be published. This systematic review also does not involve endangering participant rights. Ethical approval will not be required. The results may be published in a peer-reviewed journal or disseminated at relevant conferences. OSF REGISTRATION NUMBER: DOI 10.17605/OSF.IO/MNJAE.

Preparation and antioxidant activity of selenium nanoparticles decorated by polysaccharides from Sargassum fusiforme.

In this study, the response surface method was employed to optimize the extraction conditions of the ultrasonic-assisted extraction of Sargassum fusiforme polysaccharides (SFPS). The effects of four independent variables (hot water extraction time, ultrasonic time, ultrasonic power, and material-to-liquid ratio) on the extraction rate of SFPS were tested. In addition, the SFPS functionalized nanoselenium (SFPS-SeNPs) was prepared by chemical reduction method, whose characterization and in vitro antioxidant activity were investigated. The results showed that the yield of the crude SFPS was 25.8% at the optimal conditions of material-to-liquid ratio 1:50 (w/v), ultrasonic power 200 W, ultrasonic time 15 min, and water bath time 130 min. A series of characterization experiments showed that the SFPS-SeNPs performed higher dispersion and stability than naked SeNPs. Furthermore, the in vitro antioxidant activity assay indicated that SFPS functioned as a modifier improved the free radical scavenging activity of SeNPs significantly. In conclusion, this study provided a method to extract SFPS as a carrier for SeNPs, and SFPS-SeNPs could not only improve the stability of SeNPs, but also exerted the biological activities of SFPS. PRACTICAL APPLICATION: This research provided new ideas for the application of SFPS and the development of nanoselenium preparation carriers.

Correlation between serum vitamin B12 level and peripheral neuropathy in atrophic gastritis.

AIM: To explore the correlation between serum vitamin B12 level and peripheral neuropathy in patients with chronic atrophic gastritis (CAG). METHODS: A total of 593 patients diagnosed with chronic gastritis by gastroscopy and pathological examination from September 2013 to September 2016 were selected for this study. The age of these patients ranged within 18- to 75-years-old. Blood pressure, height and weight were measured in each patient, and the body mass index value was calculated. Furthermore, gastric acid, serum gastrin, serum vitamin and serum creatinine tests were performed, and peripheral nerve conduction velocity and Helicobacter pylori (H. pylori) were detected. In addition, the type of gastritis was determined by gastroscopy. The above factors were used as independent variables to analyze chronic gastritis with peripheral neuropathy and vitamin B12 deficiency risk factors, and to analyze the relationship between vitamin B12 levels and peripheral nerve conduction velocity. In addition, in the treatment of CAG on the basis of vitamin B12, patients with peripheral neuropathy were observed. RESULTS: Age, H. pylori infection, CAG, vitamin B9 and vitamin B12 were risk factors for the occurrence of peripheral nerve degeneration. Furthermore, CAG and H. pylori infection were risk factors for chronic gastritis associated with vitamin B12 deficiency. Serum vitamin B12 level was positively correlated with sensory nerve conduction velocity in the tibial nerve (R = 0.463). After vitamin B12 supplementation, patients with peripheral neuropathy improved. CONCLUSION: Serum vitamin B12 levels in patients with chronic gastritis significantly decreased, and the occurrence of peripheral neuropathy had a certain correlation. CAG and H. pylori infection are risk factors for vitamin B12 deficiency and peripheral neuropathy. When treating CAG, vitamin B12 supplementation can significantly reduce peripheral nervous system lesions. Therefore, the occurrence of peripheral neuropathy associated with vitamin B12 deficiency may be considered in patients with CAG. Furthermore, the timely supplementation of vitamin B12 during the clinical treatment of CAG can reduce or prevent peripheral nervous system lesions.

Randomized clinical trial of arginine-supplemented enteral nutrition versus standard enteral nutrition in patients undergoing gastric cancer surgery.

PURPOSE: Significant malnutrition exists in a high percentage of patients with gastric cancer. It is, therefore, crucial to establish an effective means to provide nutrition for these patients. This prospective, randomized, double-blinded clinical trial aims to assess the long-term survival of arginine-supplementation enteral nutrition versus standard enteral nutrition in malnourished patients with gastric cancer. METHODS: The control group (36 cases) received postoperative standard enteral nutrition. Meanwhile, the arginine-supplementation group (37 cases) adopted the same nutrition product but enriched with arginine (9.0 g/L). The primary study objective was overall survival (OS). Secondary endpoints were progression-free survival (PFS); serum parameters including total protein, albumin, proalbumin, and transferrin obtained on preoperative day 1, postoperative day 2, and day 12; CD4(+) and CD8(+) T cells, natural killer (NK) cells, immunoglobulin M (IgM), and immunoglobulin G (IgG) obtained on preoperative day 1 and postoperative day 7. RESULTS: No significant differences in baseline characteristics were observed between groups. The group receiving arginine-enriched nutrition had a significantly better OS (P = 0.03, 41 vs. 30.5 months) and better PFS (P = 0.02, 18 vs. 11.5 months). On postoperative day 7, CD4(+) T cells, NK cells, IgM and IgG levels of the arginine-supplemented group increased prominently and were significantly higher than those of the control group and those on preoperative day 1. There is no significant difference in the serum total protein, albumin, proalbumin, and transferrin levels between the two arms. CONCLUSIONS: Arginine-supplemented enteral nutrition significantly improves long-term survival and restores immunity in malnourished gastric cancer.