Sanggenon C Suppresses Tumorigenesis of Gastric Cancer by Blocking ERK-Drp1-Mediated Mitochondrial Fission.

Sanggenon C is a flavonoid extracted from the root bark of white mulberry, which is a traditional Chinese medicine with anti-inflammatory, antioxidative, and antitumor pharmacological effects. In this study, sanggenon C was found to inhibit human gastric cancer (GC) cell proliferation and colony formation, induce GC cell cycle arrest in the G0-G1 phase, and promote GC cell apoptosis. Moreover, sanggenon C was found to decrease the level of mitochondrial membrane potential in GC cells and inhibit mitochondrial fission. Mechanistically, RNA sequencing, bioinformatics analysis, and a series of functional analyses confirmed that sanggenon C inhibited mitochondrial fission to induce apoptosis by blocking the extracellular regulated protein kinases (ERK) signaling pathway, and constitutive activation of ERK significantly abrogated these effects. Finally, sanggenon C was found to suppress the growth of tumor xenografts in nude mice without obvious side effects to the vital organs of animals. This study reveals that sanggenon C could be a novel therapeutic strategy for GC treatment.

[Effect of potassium level on output and diosgenin content of Dioscorea zingiberensisg].

OBJECTIVE: To study the effect of potassium level on physiological characteristics and diosgenin content of Dioscorea zingiberensisg and provide experimental basis for proper use of potassium fertilizer. METHODS: Field experiment including four potassium levels was carried out. The physiological characteristics of leaves were determined at different growth stages and the diosgenin content in bulk root was assayed after harvest. RESULTS: Within the arrange of 0-180 kg K2SO4/hm2, the content of chlorophyll and amino acid, Fv/Fo, Fv/Fm, and phiPs II were increased with the increase in potassium level. Meanwhile, the ability to defense the damage caused by active oxygen was obviously enhanced. Further increase in potassium level resulted in the decrease in efficiency of potassium fertilizer. CONCLUSION: It indicats that higher yield and diosgenin content can be obtained when the potassium level is 180 kg K2/hm2.