Maternal VD3 supplementation during gestation improves intestinal health and microbial composition of weaning piglets.

Vitamin D3 (VD3) has been reported to improve the reproductive performance of sows. This study was conducted to investigate the long-term effect of maternal VD3 supplementation during gestation on the intestinal health of piglets. Twenty-three Landrace × Yorkshire gilts were randomly allocated into two groups to receive one of the following two diets during gestation: basal diet (CON group, 800 IU VD3 per kg diet, n = 12) and VD3 supplemented diet (VD3 group, 2000 IU VD3 per kg diet, n = 11). All sows were then fed with the same diet during lactation. Results showed that maternal VD3 supplementation during lactation tended to decrease (p = 0.08) the body weight loss of sows during lactation compared to the CON group. Besides, the relative length and weight of the small intestine (SI) and the villus height of the duodenum and ileum in weaning piglets were much higher (p < 0.05) in the VD3 group than those in the CON group, though their body weight was not changed. Meanwhile, maternal VD3 supplementation significantly upregulated the expression levels of IGF-1, IGF-2R, VDR, GLUT-2 and CAT1 in the duodenum (p < 0.05), and increased the expression levels of IGF-1, IGF-1R, IGF-2R, VDR, Occludin, ZO-1, MUC2, PEPT1 and CAT1 (p < 0.05) in the jejunum of suckling piglets compared with the CON group. Besides, the concentration of SigA in the jejunum of suckling piglets was higher (p < 0.05) in the VD3 group than that in the CON group. In addition, maternal VD3 supplementation significantly increased the contents of short chain fatty acids and the relative abundance of Lactobacillus and Faecalibacterium (p < 0.05) in the feces of weaning piglets compared to the CON group. Moreover, the relative abundance of unidentified_Lachnospiraceae in the feces of weaning piglets tended to be higher (p = 0.05), while that of unidentified_Spirochaetaceae was lower (p < 0.05) in the VD3 group than those in the CON group. Taken together, maternal VD3 supplementation during gestation could improve the intestinal function and microbiota in suckling piglets.

Maternal organic selenium supplementation alleviates LPS induced inflammation, autophagy and ER stress in the thymus and spleen of offspring piglets by improving the expression of selenoproteins.

The thymus and spleen are the main reservoir for T lymphocytes, which can regulate the innate immune response and provide protection against pathogens and tissue damage. Oxidative stress, excessive inflammation, abnormal autophagy and endoplasmic reticulum (ER) stress can all lead to dysfunction of the thymus and spleen. This study was conducted to investigate the effect of maternal 2-hydroxy-4-methylselenobutanoic acid (HMSeBA, an organic Se source) supplementation during pregnancy on the selenoprotein expression, inflammation, ER stress and autophagy of their young offspring's thymus and spleen. Thirty sows were randomly assigned to receive one of the following two diets during gestation: control diet (control, basal diet, n = 15) or HMSeBA supplemented diet (HMSeBA, basal diet +0.3 mg Se kg-1 as HMSeBA, n = 15). Tissues of thymus and spleen were collected from the offspring at birth and weaning after the lipopolysaccharide challenge. Results showed that maternal HMSeBA supplementation significantly up-regulated the gene expression of selenoproteins in the thymus and spleen of newborn piglets compared with the basal diet (p < 0.05), as well as the protein abundance of GPX1 and GPX4 (p < 0.05). In addition, maternal HMSeBA supplementation effectively decreased the expression of inflammation and autophagy related proteins in the thymus and spleen of newborn piglets as compared with the control group (p < 0.05). In weaning piglets, maternal HMSeBA significantly increased the antioxidative capacity of thymus and spleen (p < 0.05), and reversed LPS induced MDA content as compared with the control group (p < 0.05). Furthermore, maternal HMSeBA supplementation during gestation reversed the activation of the MAPK/NF-κB pathway, ER stress and autophagy induced by the LPS challenge in the thymus and spleen of weaning piglets (p < 0.05). In conclusion, maternal HMSeBA supplementation during gestation could decrease the level of inflammation, autophagy and ER stress in the thymus and spleen of young offspring by improving the antioxidative capacity and selenoprotein expression in these tissues. Therefore, maternal HMSeBA supplementation during gestation might be beneficial for the immune function of their offspring by alleviating inflammation, autophagy and ER stress levels in the thymus and spleen. This study showed more evidence for the function of Se on mater-offspring integrated nutrition.

Maternal organic selenium supplementation during gestation improves the antioxidant capacity and reduces the inflammation level in the intestine of offspring through the NF-κB and ERK/Beclin-1 pathways.

Selenium (Se) is postulated to protect against inflammation in the gut by attenuating oxidative stress. This study was conducted to investigate the effects of maternal 2-hydroxy-4-methylselenobutanoic acid (HMSeBA), an organic Se source, on the intestinal antioxidant capacity and inflammation level of the offspring and its possible mechanism. Forty-three sows were randomly assigned to receive one of the following three diets during gestation: control diet, sodium selenite (Na2SeO3) supplemented diet or HMSeBA supplemented diet, respectively. Samples were collected from the offspring at birth and weaning. The results showed that maternal HMSeBA supplementation significantly upregulated ileal GPX2 and SePP1 gene expression compared with the control and Na2SeO3 groups, while suppressed the expression of ileal IL-1ß, IL-6 and NF-κB genes in newborn piglets compared with the control group. Moreover, maternal HMSeBA supplementation significantly increased the protein of ileal GPX2 and p-mTOR compared with the control and Na2SeO3 groups, but decreased the ileal p-NF-κB, Beclin-1 and p-ERK proteins in newborn piglets compared with the control group. The weaned piglets of the HMSeBA group had lower serum IL-1ß and IL-6 than the piglets of the control group at 2 h of LPS challenge. In addition, after the LPS challenge, the HMSeBA group had a lower relative abundance of ileal p-NF-κB and Beclin-1 proteins than the control and Na2SeO3 groups. In conclusion, maternal HMSeBA supplementation during gestation can improve the offspring's intestinal antioxidant capacity and reduce the inflammation level by suppressing NF-κB and ERK/Beclin-1 signaling.

Effect of maternal organic selenium supplementation during pregnancy on sow reproductive performance and long-term effect on their progeny.

Selenium (Se) is an essential trace element for animals and exists in nature in both inorganic and organic forms. Although organic Se is more bioavailable than inorganic Se, there are inconsistent reports on the effect of organic Se on the reproductive performance of sows. This study was conducted to investigate the effect of maternal organic Se (2-hydroxy-4-methylselenobutanoic [HMSeBA]) supplementation on reproductive performance and antioxidant capacity of sows, and the long-term effect on the growth performance and antioxidant capacity of their offspring with or without lipopolysaccharide (LPS) challenge. The experimental design used in this study was a completely randomized design; 45 Landrace × Yorkshire sows were randomly allocated to receive one of the following three diets during gestation: control diet (Control, basal diet, n = 15), sodium selenite (Na2SeO3)-supplemented diet (Na2SeO3, basal diet + 0.3 mg Se/kg Na2SeO3, n = 15), and HMSeBA-supplemented diet (HMSeBA, basal diet + 0.3 mg Se/kg HMSeBA, n = 15). On day 21 of age, male offspring from each group were injected with LPS or saline (n = 6). As compared with the control group, maternal HMSeBA supplementation increased the number of total born piglets, while decreased birth weight (P < 0.05). In the first week of lactation, maternal HMSeBA supplementation increased litter weight gain compared with the Na2SeO3 group (P < 0.05) and increased the average daily gain of piglets compared with the control group and Na2SeO3 group (P < 0.05). Meanwhile, maternal HMSeBA supplementation decreased piglet birth interval as compared with the control group and Na2SeO3 group (P < 0.05). Besides, plasma glutathione peroxidase (GSH-Px) activity was higher in the HMSeBA group on farrowing 0 min and 90 min, while malondialdehyde (MDA) concentration was lower on farrowing 0, 90, and 135 min than those in the control group (P < 0.05). In addition, maternal HMSeBA supplementation increased the concentration of selenoprotein P (SELENOP) in colostrum compared with the control group (P < 0.05). Further study revealed that the LPS-challenged HMSeBA group had higher GSH-Px and total antioxidant capacity and lower MDA in weaning piglets compared with the LPS-challenged control group (P < 0.05). Taken together, maternal HMSeBA supplementation increased the number of total born piglets, shortened the duration of farrowing, improved the antioxidant capacities of sows and their offspring, and improved the growth performance of suckling pigs at the first week of lactation. Thus, HMSeBA supplementation during gestation has the potentiality to produce more kilogram of meat.