[Effect of auricular-point pressure combined with personalized music therapy on perioperative stress response in primipara].

OBJECTIVE: To explore the effect of auricula-point pressure combined with personalized music therapy on perioperative stress response in primipara. METHODS: A total of 1000 primiparas who were elective for cesarean section were randomly divided into an observation group and a control group, 500 cases in each one. 1 h before surgery and 3 h, 6 h, 12 h and 24 h after surgery, individualized music relaxation intervention was given for 1 h in the two groups. On the basis of the treatment, auricular-point pressure was added at Pizhixia (AT4), Shenmen (TF4), Xin (CO15), Zigong (uterus), Luanchao (ovary), Neishengzhiqi (TF2) in the observation group. Each point was pressed for 1 min each time, repeated once every 15 min, the force is from light to heavy, and it is better to have a feeling of distension, sourness and radiation in the auricle. The scores of anxiety self-rating scale, visual analog scale (VAS), heart rate, systolic blood pressure, thyroid stimulating hormone, cortisol and blood glucose levels were observed in the two groups. RESULTS: A total of 973 primiparas completed the clinical study in the end, including 488 patients in the observation group and 485 patients in the control group. Compared to enrollment, the scores of anxiety self-rating scale before entering the operating room in the two groups were significantly improved (P<0.05), and the observation group was superior to the control group (P<0.05). Entering the operating room and tracheal extubation, the heart rate and systolic blood pressure of the primiparas in the observation group were more stable than those in the control group, and the differences were statistically significant (P<0.05). Entering the operating room, 1 h into the surgery and 3 h after surgery, the thyroid stimulating hormone, cortisol and blood glucose levels of the primiparas in the observation group were significantly better than those in the control group (P<0.05). The VAS scores in the observation group at 6 h, 12 h and 24 h after surgery were lower than those in the control group (P<0.05). CONCLUSION: Auricular-point pressure combined with personalized music therapy can reduce the perioperative stress response in primipara and promote the improvement of psychological, physiological and emotional.

Magnetic beads-based neuraminidase enzyme microreactor as a drug discovery tool for screening inhibitors from compound libraries and fishing ligands from natural products.

Neuraminidase (NA) is a glycoside hydrolase that has been proposed as a potential therapeutic target for influenza. Thus, the identification of compounds that modulate NA activity could be of great therapeutic importance. The aim of this study is to develop a drug discovery tool for the identification of novel modulators of NA from both compound libraries and natural plant extracts. NA was immobilized onto the surface of magnetic beads and the inherent catalytic activity of NA-functionalized magnetic beads was characterized. Based on the enzymatic activity (hydrolysis ratio), the inhibitory activities of 12 compounds from plant secondary metabolites were screened, and the desired anti-NA activities of flavonoids were certified. Ligand fishing with the immobilized enzyme was optimized using an artificial test mixture consisting of oseltamivir, lycorine and matrine prior to carrying out the proof-of-concept experiment with the crude extract of Flos Lonicerae. The combination of ligand fishing and HPLC-MS/MS identified luteolin-7-O-ß-D-glucoside, luteolin, 3,5-di-O-caffeoylquinic acid and 3,4-di-O-caffeoylquinic acid as neuraminidase inhibitory ligands in Flos Lonicerae. This is the first report on the use of neuraminidase functionalized magnetic beads for the identification of active ligands from a botanical matrix, and it sets the basis for the de novo identification of NA modulators from complex biological mixtures.

[The effect of Duraphat varnish on fluoride content of enamel in deciduous teeth].

PURPOSE: The aim of this study was to analyse the fluoride distribution in deciduous teeth enamel after using Duraphat varnish,and to supply the evidence for preventing deciduous caries by using Duraphat varnish. METHODS: After coating Duraphat varnish, the alteration of fluoride distribution in the enamel was observed with electron probe micro-analysis. The result was compared with the control group and NaF solution group. The effect of dose and period of coating Duraphat varnish on fluoride distribution in enamel was evaluated by X-ray photo-electron spectrometer(XPS). The data was analyzed with SPSS17.0 software package. RESULTS: The mean values of fluoride concentration in the control group, NaF group and Duraphat varnish group were(0.0406±0.0234)wt%,(0.1006±0.1040)wt% and (0.1844±0.1293)wt%,respectively.The concentration of fluoride in the Duraphat varnish group was significantly higher than that in the NaF group (P<0.01). In period-dependent test,the mean values of fluoride concentration at 10µm under the enamel surface of short period group and long period group were(0.2407±0.0034)wt%, (0.1434±0.0133)wt%; The concentration of fluoride in the short period group was significantly higher than that in the long period group(P<0.01). In dose-dependent test, the mean values of fluoride concentration at 10µm under the enamel surface in the low-dose group and high-dose group were(0.4417±0.0034)wt%,(0.4413±0.0044)wt%, the concentration of fluoride in the low-dose group and high-dose group had no significant difference(P>0.05). CONCLUSIONS: It is an effective way to increase the concentration of fluoride in enamel by coating Duraphat varnish on the surface of deciduous teeth. Shorten the coating period could increase the concentration of fluoride in enamel effectively but the concentration of fluoride in enamel does not go up along with the coating dose increase.

[Screening of effective fraction on experimental gastric ulcer from Pongamia pinnata roots].

OBJECTIVE: To study the effect of extract from Pongamia pinnata roots on experimental gastric ulcer and screen the effective fraction. METHOD: The models of gastric mucosa damage were induced by absolute alcohol in rats and reserpine in mice to observed the effect of ethyl alcohol extract from P. pinnata roots (PRE) and different parts on experimental gastric ulcer. RESULT: PRE, acetic ether extract and n-butanol extract could significantly inhibit the gastric mucosa damage induced by absolute alcohol in rats and reserpine in mice. In absolute alcohol models the gastric ulcer rates of inhibition were 86.4%, 85.4%, 11.5%, respectively. In reserpine models the gastric ulcer rates of inhibition were 37.8%, 33.8%, 19.7%, respectively. CONCLUSION: PRE, acetic ether extract and n-butanol extract could significantly inhibit the gastric mucosa damage induced by absolute alcohol in rats and reserpine in mice. Acetic ether extract from P. pinnata roots has the best effect on experimental gastric ulcer.