RESUMO
The effect of Paeonia lactiflora (PL) on platelet cytosolic free calcium and erythrocyte membrane Ca(2+)-Mg(2+)-ATPase activity in hyperlipid rabbits were observed. Results showed the level of platelet cytosolic free calcium in the PL group (276.25 +/- 27.00 nmol/L) was significantly lower than that in the cholesterol group (390.88 +/- 70.00 nmol/L), P < 0.01, the basal and calmodulin-stimulated activities of erythocyte membrane Ca(2+)-Mg(2+)-ATP ase in PL group (0.79 +/- 0.05 mumol.pi-1.mg-1.h-1 and 1.34 +/- 0.10 mumol.pi-1.mg-1.h-1) were higher than that in the cholesterol group (0.65 +/- 0.09 mumol.pi-1.mg-1.h-1 and 1.04 +/- 0.13 mumol.pi-1.mg-1.h-1).
Assuntos
Plaquetas/metabolismo , ATPase de Ca(2+) e Mg(2+)/sangue , Cálcio/sangue , Membrana Eritrocítica/enzimologia , Hipercolesterolemia/sangue , Animais , Medicamentos de Ervas Chinesas/farmacologia , Masculino , Coelhos , Distribuição AleatóriaAssuntos
Aminoidrolases/metabolismo , Fabaceae/enzimologia , Formiato-Tetra-Hidrofolato Ligase/metabolismo , Leucovorina/análogos & derivados , Metilenotetra-Hidrofolato Desidrogenase (NADP)/metabolismo , Plantas Medicinais , Aminoidrolases/isolamento & purificação , Cromatografia , Cromatografia de Afinidade , Cromatografia DEAE-Celulose , Cromatografia em Gel , Durapatita , Cinética , Leucovorina/metabolismo , Meteniltetra-Hidrofolato Cicloidrolase , Metilenotetra-Hidrofolato Desidrogenase (NADP)/isolamento & purificaçãoRESUMO
This paper describes that the absorption spectrum of bilirubin solution was changed obviously by photo-oxidation. The absorbance was measured at 453 nm before and after light irradiation. The contents of bilirubin of ox gallstone in Liuying pills, Liushen pills and Niuhuang Xiaoyan pills were determined by differential absorbance (delta A). Calibration graph was linear in the range of 1.6-8.0 micrograms/ml for delta A. The average recoveries for three Chinese patent medicines were over 93%. Determinations of bilirubin in Chinese patent medicines were not affected by other components.