RESUMO
As a rare endangered medical plant that newly cultivated,little experimental information is available for growth and metabolites of Tetrastigma hemsleyanum in response to nitrogen( N). The effects of different levels of N on growth of T. hemsleyanum and the content of phytochemicals( polysaccharide,total flavonoids and phenolics) and antioxidant activity( ABTS and FRAP) in stems and leaves were investigated in this study. A certain amount of N had positive effects on most of biological traits,and excessive dose of N went against growth of T. hemsleyanum. With N levels decreased,the polysaccharide content in stems and leaves had no significant change,while the total flavonoid and phenolic content,and antioxidant activities increased steadily. Antioxidant activities and total flavonoid and phenolic content had significant positive correlation. Excessive N fertilizer should be avoided by cultivation.
Assuntos
Vitaceae , Antioxidantes , Flavonoides , Nitrogênio , Fenóis , Compostos Fitoquímicos , Extratos Vegetais , Folhas de PlantaRESUMO
The upregulation of both HSP70 and HSP90 frequently compromises the effects of thermotherapy. The co-inhibition of HSP70/HSP90 may be preferable to enhance the effects of thermotherapy on nasopharyngeal carcinoma cells. The changes of HSP70 and HSP90 were detected after thermotherapy in human nasopharyngeal cancer cell HNE1. 17-Dimethylaminoethylamino-17-demethoxygeldanamycin (17-DMAG) and quercetin were used to inhibit the activity of HSP90 and HSP70. The enhanced effects were evaluated in vitro and in vivo. Both HSP70 and HSP90 were upregulated promptly in HNE1 after thermotherapy. Single inhibition of HSP70 resulted in overexpression and delayed descent of HSP90. The co-inhibition of HSP70/HSP90 with quercetin plus 17-DMAG significantly increased apoptosis in hyperthermia-treated HNE1 cells both in vitro and in vivo. The co-inhibition of HSP70/HSP90 synergistically sensitizes nasopharyngeal carcinoma cells to hyperthermia.
Assuntos
Benzoquinonas/farmacologia , Proteínas de Choque Térmico HSP70/antagonistas & inibidores , Proteínas de Choque Térmico HSP90/antagonistas & inibidores , Hipertermia Induzida/métodos , Lactamas Macrocíclicas/farmacologia , Neoplasias Nasofaríngeas/terapia , Quercetina/farmacologia , Apoptose/efeitos dos fármacos , Apoptose/genética , Carcinoma , Linhagem Celular Tumoral , Terapia Combinada , Sinergismo Farmacológico , Proteínas de Choque Térmico HSP70/genética , Proteínas de Choque Térmico HSP70/metabolismo , Proteínas de Choque Térmico HSP90/genética , Proteínas de Choque Térmico HSP90/metabolismo , Humanos , Carcinoma Nasofaríngeo , Neoplasias Nasofaríngeas/genética , Neoplasias Nasofaríngeas/metabolismo , Neoplasias Nasofaríngeas/patologia , Regulação para Cima/efeitos dos fármacosRESUMO
OBJECTIVE: To investigate the changes and effects of HSP70/HSP90 of in nasopharyngeal carcinoma cells HNE1 after thermotherapy. METHODS: HNE1 cells were incubated at 42 degrees C for 2 h. The changes of mRNA and protein level of HSP70/HSP90 were detected by real-time PCR and western-blot at different intervals. HNE1 cells were pretreated with quercetin, geldanamycin or quercetin plus geldanamycin, respectively, before heat treatment. Flow cytometry assay was applied to determine the apoptosis of HNE1 cells before thermotherapy and at 2 h, 4 h, 6 h, 8 h, 12 h and 24 h after thermotherapy. RESULTS: HSP70/HSP90 expression was up regulated at 2 h, reached to its peak at 4 h, descended at 8 h and returned to the normal level at 24 h after thermotherapy. The inhibition of HSP70 through quercetin induced up-regulation and delayed descent of HSP90. Combined pretreatment with quercetin and geldanamycin could significantly induce HNE1 apoptosis when compared with pretreatment with quercetin or geldanamycin alone (P<0.05). CONCLUSION: HSP70/HSP90 expression in HNE1 up regulated promptly after thermotherapy. Inhibition of expression and activity of HSP70/HSP90 before thermotherapy can increase sensitivity of the tumor cell to heat treatment.