Therapeutic effect of gene silencing peptidyl arginine deaminase 4 on pulmonary interstitial lesions induced by collagen-induced arthritis mice / 北京大学学报(医学版)

OBJECTIVE@#To investigate the therapeutic effect of gene silencing peptidyl arginine deaminase 4 (PAD4) on pulmonary interstitial lesions induced by collagen-induced arthritis (CIA) mice, and possible mechanisms.@*METHODS@#A CIA mouse model was established in DBA/1 mice, followed by a tail vein injection of the virus solution prepared by the PAD4-siRNA expression vector once a week for 8 times. The mice were sacrificed at the end of the experiment. The expression of PAD4 mRNA in lungs was detected by real-time quantitative PCR (qRT-PCR). The expression of PAD4 protein was detected by tissue immunohistochemistry. Cell culture was performed by spleen tissue. Flow cytometry changes in the ratio of Tfh cells to Tfr cells were examined; lung staining was performed in the lungs to observe changes in lung pathology.@*RESULTS@#(1) Compared with the blank group, the expression of PAD4 mRNA in the lung tissue of the model group increased, the difference was statistically significant (P < 0.05). PAD4 mRNA in the lung tissue of the CIA mice after PAD4-siRNA treatment. The expression level was significantly lower than that of the model group and the negative control group, and the difference was statistically significant (P < 0.05). (2) Red fluorescence was less in the lung tissue of the blank group, while more red fluorescence was observed in the inflammatory cell infiltration area and trachea around the lung tissue of the model group and the negative control group, and the red fluorescence of the three groups after PAD4-siRNA treatment was significantly reduced; (3) Compared with the blank group, the proportion of Tfh cells in the model group increased, the difference was statistically significant (P < 0.05), the proportion of Tfh cells in spleen cells of the CIA mice after PAD4-siRNA treatment was significantly lower than that of the model group and the negative control group, the difference was statistically significant (P < 0.05); compared with the blank group, in the mouse spleen cells in the model group the proportion of Tfr cells was slightly decreased, but the difference was not statistically signifi-cant. The proportion of Tfr cells in the spleen cells of the mice increased after PAD4-siRNA treatment, but the difference was statistically significant only in the PAD4-siRNA2 group compared with the model group and the negative control group (P < 0.05); (4) The proportion of Tfh/Tfr in the spleen cells of the model group was increased, compared with the blank group, the difference was statistically significant (P < 0.05); the ratio of Tfh/Tfr in the three groups after PAD4-siRNA treatment all decreased, the difference was statistically significant (P < 0.05); (5) Compared with the blank group, the alveolar wall of the lung tissue of the model group was thickened, the inflammatory cell infiltration was increased, and the lung tissue destruction and inflammatory infiltration of the CIA mice were decreased after PAD4-siRNA treatment. The degree of reduction was reduced.@*CONCLUSION@#Gene silencing of PAD4 can reduce the proportion of Tfh cells, increase the proportion of Tfr cells, reverse the proportion of Tfh/Tfr, and reduce the degree of interstitial lesions and inflammatory infiltration of lung tissue.

Response rules of pressing pain on back section in Governor Vessel in patients with gastroesophageal reflux disease / 中国针灸

The response rules of pressing pain on the back section in the Governor Vessel in patients with gastro-esophageal reflux disease (GERD) were studied to provide references for the diagnosis and treatment of GERD. Seventy-six cases of GERD were included into an observation group while 30 healthy volunteers were recruited into a control group. A mechanical measurement device of pressing pain that could measure the pain threshold was adapted to observe the pressing pain on the back section in the Governor Vessel in GERD patients and healthy volunteers. The test area is from spinous process of the 1st thoracic vertebra to that of the 12th thoracic vertebra (T1 -T12), including acupoints and non-acupoints on the Governor Vessel. As a result, in the observation group the pain threshold of T5-T7 spinous process clearance, which was the location of Shendao (GV 11), Lingtai (GV 10) and Zhiyang (GV 9), was lower than that in the control group (all P < 0.05). This result indicated that there was significant pressing pain in T5-T7 spinous process clearance in patients with GERD, which could be taken as an important auxiliary diagnosis and a new thinking method in the treatment of GERD with acupuncture.

Effects of the needling method for regulating kidney and smoothing liver on endocrine and immune functions in the patient with hyperplasia of mammary glands / 中国针灸

<p><b>OBJECTIVE</b>To probe into a better acupoint association for treatment of hyperplasia of mammary glands and the mechanism.</p><p><b>METHODS</b>Two hundred cases were randomly divided into a regulating kidney and smoothing liver needling group, a routine acupuncture group and a Chinese medicine group. The regulating kidney and smoot hing liver needling group were treated with acupuncture at Shenshu (BL 23), Mingmen (GV 4), Fengfu (GV 16), Lingxu (KI 24), Shenfeng (KI 23), Bulang (KI 22), Qimen (LR 14), etc. and the routine acupuncture group with acupuncture at Zusanli (ST 36), Tanzhong (CV 17), Ganshu (BL 18), Rugen (ST 18), Neiguan (PC 6), Qimen (LR 14), once daily; and the Chinese medicine group with oral administration of Rupixiao tablet, 6 tablets each time, thrice each day. After they were treated for 2 menstrual cycles, the therapeutic effects and changes of E2, PRL, P, CD3+, CD4+ and CD8+ were observed.</p><p><b>RESULTS</b>The clinical therapeutic effect and the total effective rate for TCM syndromes were 92.0% and 94.0% in the regulating kidney and smoothing liver needling group, 86.0% and 88.0% in the routine acupuncture group and 78.0% and 84.0% in the Chinese medicine group, with a significant difference among the 3 groups, the former being better than the other two groups; after treatment, serum PRL, P, P/E2 significantly improved in all the 3 groups (P < 0.05) and the regulating kidney and smoothing liver needling group was better than other two groups in decreasing PRL and increasing P, P/E2 (P < 0.05); and E2, CD8+, CD4+/CD8+ significantly improved in the regulating kidney and smoothing liver needling group and the routine acupuncture group, the former being better than the later in decreasing E2 (P < 0.05).</p><p><b>CONCLUSION</b>The regulating kidney and smoothing liver needling method is an effective method for treatment of hyperplasia of mammary glands and it has regulative action on endocrine and immune functions in the patient with hyperplasia of mammary glands.</p>