RESUMO
Intimal hyperplasia-induced restenosis is a common response to vascular endothelial damage caused by mechanical force or other stimulation, and is closely linked to vascular remodeling. Curcumin, a traditional Chinese medicine, exhibits potent protective effects in cardiovascular diseases; for example, it attenuates vascular remodeling. Although the suppressive effects of curcumin on diseases caused by vascular narrowing have been investigated, the underlying mechanisms remain unknown. Long non-coding RNAs (lncRNAs) regulate various pathological processes and affect the action of drugs. In the present study, we found that the curcumin remarkably downregulated the expression of lncRNA H19 and thereby inhibited intimal hyperplasia-induced vascular restenosis. Furthermore, the inhibition of the expression of H19 by curcumin resulted in the inactivation of the Wnt/ß-catenin signaling. Overall, we show that curcumin suppresses intimal hyperplasia via the H19/Wnt/ß-catenin pathway, implying that H19 is a critical molecule in the suppression of intimal hyperplasia after balloon injury by curcumin. These insights should be useful for potential application of curcumin as a therapeutic intervention in vascular stenosis.
Assuntos
Estenose das Carótidas/tratamento farmacológico , Curcumina/farmacologia , RNA Longo não Codificante/metabolismo , Remodelação Vascular/efeitos dos fármacos , Via de Sinalização Wnt/genética , Animais , Artérias Carótidas/patologia , Estenose das Carótidas/genética , Estenose das Carótidas/patologia , Linhagem Celular , Curcumina/uso terapêutico , Modelos Animais de Doenças , Técnicas de Silenciamento de Genes , Humanos , Masculino , RNA Longo não Codificante/genética , Ratos , Remodelação Vascular/genética , Via de Sinalização Wnt/efeitos dos fármacosRESUMO
A new seco-friedelolactone, named itoaic acid, together with 5 known compounds was isolated from the bark and twigs of Itoa orientalis. The structure was elucidated by means of MS, 1D and 2D NMR techniques. Anti-inflammatory activity against COX-2 was evaluated for several compounds from I. orientalis and another Flacourtiaceae plant Xylosma controversum.
Assuntos
Anti-Inflamatórios/isolamento & purificação , Ácido Oleanólico/análogos & derivados , Extratos Vegetais/química , Salicaceae/química , Anti-Inflamatórios/química , Anti-Inflamatórios/farmacologia , Ciclo-Oxigenase 2/metabolismo , Estrutura Molecular , Ácido Oleanólico/isolamento & purificação , Ácido Oleanólico/farmacologia , Casca de Planta , Extratos Vegetais/farmacologia , Caules de PlantaRESUMO
Investigations of two Flacourtiaceae plants, Bennettiodendron leprosipes and Flacourtia ramontchi, resulted in the isolation and structural elucidation of six new constituents including two phenolic glycosides ( 1 and 2), one lignan ( 3), two lignan glycosides ( 4 and 5), and a monoterpene glycoside ( 6), together with 22 known compounds ( 7- 28). The structures of the new compounds were elucidated by spectroscopic analysis and chemical methods. The selected isolates 1, 2, 8- 10, 22- 26, and some phenolic glycosides 29- 42 previously isolated from another Flacourtiaceae plant, Itoa orientalis, were tested against snake venom phosphodiesterase I (PDE I) activity. The result indicated that 22, 30, 32, 34, and 40 exhibited moderate inhibitory activities against PDE I with the values ranging from 13.15 to 20.86 %, and 1, 8, 10, 25, 31, 33, 35, 38, 39, and 41 showed weak inhibitory activity.