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1.
J Nanobiotechnology ; 19(1): 261, 2021 Sep 04.
Artigo em Inglês | MEDLINE | ID: mdl-34481495

RESUMO

BACKGROUND: Chemodynamic therapy (CDT), employing Fenton or Fenton-like catalysts to convert hydrogen peroxide (H2O2) into toxic hydroxyl radicals (·OH) to kill cancer cells, holds great promise in tumor therapy due to its high selectivity. However, the therapeutic effect is significantly limited by insufficient intracellular H2O2 level in tumor cells. Fortunately, ß-Lapachone (Lapa) that can exert H2O2-supplementing functionality under the catalysis of nicotinamide adenine dinucleotide (phosphate) NAD(P)H: quinone oxidoreductase-1 (NQO1) enzyme offers a new idea to solve this problem. However, extensive DNA damage caused by high levels of reactive oxygen species can trigger the "hyperactivation" of poly(ADP-ribose) polymerase (PARP), which results in the severe interruption of H2O2 supply and further the reduced efficacy of CDT. Herein, we report a self-amplified nanocatalytic system (ZIF67/Ola/Lapa) to co-deliver the PARP inhibitor Olaparib (Ola) and NQO1-bioactivatable drug Lapa for sustainable H2O2 production and augmented CDT ("1 + 1 + 1 > 3"). RESULTS: The effective inhibition of PARP by Ola can synergize Lapa to enhance H2O2 formation due to the continuous NQO1 redox cycling. In turn, the high levels of H2O2 further react with Co2+ to produce the highly toxic ·OH by Fenton-like reaction, dramatically improving CDT. Both in vitro and in vivo studies demonstrate the excellent antitumor activity of ZIF67/Ola/Lapa in NQO1 overexpressed MDA-MB-231 tumor cells. Importantly, the nanocomposite presents minimal systemic toxicity in normal tissues due to the low NQO1 expression. CONCLUSIONS: This design of nanocatalytic system offers a new paradigm for combing PARP inhibitor, NQO1-bioactivatable drug and Fenton-reagents to obtain sustained H2O2 generation for tumor-specific self-amplified CDT.


Assuntos
Antineoplásicos/farmacologia , Nanoestruturas/química , Nanoestruturas/uso terapêutico , Neoplasias de Mama Triplo Negativas/tratamento farmacológico , Animais , Apoptose , Linhagem Celular Tumoral , Dano ao DNA/efeitos dos fármacos , Humanos , Peróxido de Hidrogênio/metabolismo , Camundongos , NAD(P)H Desidrogenase (Quinona) , Nanopartículas , Naftoquinonas , Poli(ADP-Ribose) Polimerase-1 , Espécies Reativas de Oxigênio/metabolismo
2.
Environ Pollut ; 290: 118002, 2021 Dec 01.
Artigo em Inglês | MEDLINE | ID: mdl-34419862

RESUMO

The influence of intercalated anions on the structure and composition of green rusts supplies a theoretical possibility for the investigation of the structural modification of FeII/FeIII (oxyhydr)oxide materials. ß-Cyclodextrin was intercalated into the mixed-valent iron-based hydroxide layers to synthesize new green rust materials (ß-CD GRs), pursuing high-capacity uraniumVI (UVI) sorption. The molar ratios of FeII to FeIII and the molar ratios of ß-CD GR to FeII + FeIII had a significant effect on the synthesis of ß-CD GRs. The synthesis process was further optimized by the quadric predictor and desirability function in a central composite design in combination. Both strong acidity and alkalinity were harmful to the adsorption of ß-CD GRs towards UVI. The pseudo-first-order kinetic model and Langmuir isotherm model were appropriate in fitting the whole adsorption process. The maximum monolayer adsorption capacity of ß-CD GRs was 2548.61 mg/g. The presence of mimic groundwater constituents explicitly deteriorated the interaction between ß-CD GR and UVI species. Nanoscale nodules and particles were formed on the ß-CD GR after the adsorption experiments. The peaks at 1159 and 609 cm-1 vanished with the band at 1103 cm-1 being left-shifted to 1117 cm-1 in the FTIR spectra of ß-CD GR during the heterogeneous process. The intercalation of ß-CD brought obvious enhancement of UVI species sorption to the GR material, which was combinedly driven by several reaction pathways and different from the unmodified GRs.


Assuntos
Urânio , beta-Ciclodextrinas , Adsorção , Compostos Férricos , Ferro , Cinética
3.
J Med Food ; 24(2): 116-123, 2021 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-33523769

RESUMO

This study focused on the antibacterial effects of the endophytic fungi producing naringenin from Dalbergia odorifera T. Chen against Staphylococcus aureus. The antibacterial activity was measured by the inhibition diameters, minimum inhibitory concentration (MIC), and minimum bactericidal concentration (MBC). The time-killing curve was also used to evaluate its antibacterial efficacy. The results of antibacterial activity determinations showed that endophytic fungi secondary metabolites can inhibit the growth of five pathogenic bacteria (S. aureus, Escherichia coli, Salmonella enteritidis, Pseudomonas aeruginosa, and Bacillus subtilis) and the most sensitive strain was S. aureus that had the MIC and MBC values of 0.13 and 0.50 mg/mL, respectively. The membrane permeability study was measured by a DNA leakage assay and electrical conductivity assay. Furthermore, the whole-cell protein lysates and DNA fragmentation assay was evaluated. The morphology of S. aureus treated with the endophytic fungi products was observed by scanning electron microscopy (SEM). The probable antibacterial mechanism of endophytic fungi secondary metabolites was the increased membrane permeability that leads to leaks of nucleic acids and proteins. SEM results further confirmed that the extracts can interfere with the integrity of S. aureus cell membrane and further inhibit the growth of bacteria, resulting in the death of bacteria. This study provides a new perspective for the antibacterial functions of endophytic fungi secondary metabolites for biomedical applications.


Assuntos
Proteínas de Bactérias , Membrana Celular , DNA Bacteriano , Dalbergia , Flavanonas , Fungos , Staphylococcus aureus , Antibacterianos/farmacologia , Proteínas de Bactérias/efeitos dos fármacos , Membrana Celular/efeitos dos fármacos , DNA Bacteriano/efeitos dos fármacos , Dalbergia/microbiologia , Flavanonas/farmacologia , Fungos/química , Testes de Sensibilidade Microbiana , Staphylococcus aureus/efeitos dos fármacos
4.
Ecotoxicol Environ Saf ; 213: 112003, 2021 Apr 15.
Artigo em Inglês | MEDLINE | ID: mdl-33588188

RESUMO

A green rust-coated expanded perlite (GR-coated Exp-p) microelectrode was synthesized and incorporated into a column-mode three-dimensional electrokinetic (3D-EK) platform to effectively pursue a continuous Cr(VI) removal from the aqueous solution. Brucite-like layers of GR were decorated onto the Exp-p material. The molar ratio of Fe(II) to Fe(III) played a most vital role among the three synthesis factors in influencing the performance of the particle electrode. For the equilibrium adsorption experiments, the target maximum adsorption capacity of 122 mg/g was predicted by a target optimizer and desirability function at the conditions following the pH of 4.7, the initial concentration of 172.4 mg/L, the dosage of 0.28 g/L, and the temperature of 28.96 °C, respectively. SO42-, Cl-, and NO3- fiercely competed with Cr(VI) anions in the acidic conditions for the locally positive sites. A low concentration and a slow flow were favored in the column-mode 3D-EK platform. The pseudo-first-order and Langmuir models were suitable for describing the kinetics and isotherms of the adsorption process, respectively. Cr(VI) anions were electrostatically attracted to the silanol groups and GR surface of the adsorbent, subsequently reduced in both heterogeneity and homogeneity, and finally immobilized by coordinating with silanediol groups and silanetriol groups.


Assuntos
Cromo/química , Poluentes Químicos da Água/química , Purificação da Água/métodos , Adsorção , Óxido de Alumínio , Ânions , Eletrodos , Compostos Férricos , Concentração de Íons de Hidrogênio , Cinética , Dióxido de Silício , Temperatura , Água , Poluentes Químicos da Água/análise
5.
Nanoscale ; 12(41): 21234-21247, 2020 Oct 29.
Artigo em Inglês | MEDLINE | ID: mdl-33063070

RESUMO

A combination of chemotherapy and phototherapy has been proposed as a promising treatment for esophageal cancer (EC). Irinotecan as a first-line treatment option is widely prescribed for metastatic EC, however, its clinical application is extremely restricted by the low conversion rate to SN38, severe myelosuppression and diarrhea. As a more potent active metabolite of irinotecan, SN38 is a better substitution for irinotecan, but the poor water solubility and the difficulty of encapsulation hindered its medical application. Herein, a multifunctional SN38-conjugated nanosystem (FA-PDA@PZM/SN38@BSA-MnO2, denoted as FA-PPSM) is designed for overcoming the above-mentioned drawbacks and achieving collaborative chemotherapy, photodynamic therapy (PDT) and photothermal therapy (PTT). The tumor acidic microenvironment induces decomposition of BSA-MnO2 nanoparticles into O2 and Mn2+, thus enhancing oxygen-dependent PDT efficacy; meanwhile, Mn2+ can be employed as a magnetic resonance imaging (MRI) contrast agent. Under 650 and 808 nm laser irradiation, the FA-PPSM nanocomposites exhibit superior antitumor efficacy in Eca-109-tumor bearing mice. Notably, there is low gastrointestinal toxicity and myelosuppression in the FA-PPSM treated mice compared with those treated with irinotecan (alone). Taken together, this work highlights the great potential of the FA-PPSM nanocomposites for MRI-guided chemotherapy in combination with endoscopic light therapy for esophageal cancer.


Assuntos
Neoplasias Esofágicas , Nanopartículas , Animais , Linhagem Celular Tumoral , Diarreia , Neoplasias Esofágicas/tratamento farmacológico , Irinotecano , Compostos de Manganês , Camundongos , Óxidos , Fototerapia , Microambiente Tumoral
6.
Nanoscale ; 12(37): 19149-19158, 2020 Oct 01.
Artigo em Inglês | MEDLINE | ID: mdl-32936174

RESUMO

The rampant usage of antibiotics has led to the emergence of toxicity, especially hepatotoxicity and the emergence of microbial drug resistance. Hence, a series of novel hepatoprotective, biocompatible, antibacterial silver nanoformulations (AS-AgNPs) were developed by using the important Chinese medicinal plant Angelica sinensis. The different size of AS-AgNPs were characterized by UV-Visible spectroscopy, transmission electron microscopy (TEM), scanning electron microscopy (SEM), X-ray diffraction (XRD) and Fourier transform infrared spectroscopy (FT-IR). The size-dependent antibacterial properties of AS-AgNPs were investigated against Gram-positive, Gram-negative and multi-drug resistant bacteria. The minimum inhibitory concentration (MIC) of AS-AgNPs with different size against six bacteria was found to be in the range of 5-100 µg mL-1 with no resistance till 12 cycles. TEM and SEM results of bacteria after the treatment suggested that AS-AgNPs disrupted the cell membrane by creating pores. The cytocompatibility and cytoprotective effect of AS-AgNPs were evaluated against HepG2 cell lines, which showed that 85% of cells were viable up to 100 µg mL-1 of the concentration with almost no change in AST and ALT levels. Further, a logic combinatorial library, including basic logic gates (AND, OR, NOR, INHIBIT, IMPLICATION, and YES), three input logic gates (OR, and NOR) and combinatorial gates (INH-OR, INH-YES, INH-INH, AND-NOR, and NOT-AND-NOR) were designed by integrating multi-components based on the interaction between AS-AgNP1 and bacteria, where DiSC3(5) was used as the signal reporter. This system clearly demonstrates the ability of simple logic circuits to perform sophisticated analysis for the detection of multiple bacteria.


Assuntos
Angelica sinensis , Nanopartículas Metálicas , Antibacterianos/farmacologia , Bactérias , Testes de Sensibilidade Microbiana , Extratos Vegetais/farmacologia , Prata , Espectroscopia de Infravermelho com Transformada de Fourier
7.
Commun Biol ; 3(1): 343, 2020 07 03.
Artigo em Inglês | MEDLINE | ID: mdl-32620811

RESUMO

Despite its great potential in cancer therapy, phototherapy, including photothermal therapy (PTT) and photodynamic therapy (PDT), often cause metastasis of tumors. Immunotherapy has revolutionized the cancer treatment owing to the capability of activating immune system to eliminate tumors. However, the integration of phototherapy and immunotherapy in a single nanoagent for cancer therapy is still a challenging task. Here, we fabricated (Cu9S5@mSiO2-PpIX@MnO2@CpG (CSPM@CpG)) as a synergistic therapeutic model for phototherapy enhanced immunotherapy. The intracellular uptake of cytosine-phosphate-guanine (CpG) promoted the infiltration of cytotoxic T lymphocytes (CTLs) in tumor tissue, further stimulating the production of interferon gamma (IFN-γ) and remarkably elevating the immune response level. Excellent anti-tumor effects have been achieved by synergistic PTT/PDT/immunotherapy. The metastasis of tumors was effectively inhibited by the immune response of CpG. Thus, our proposed work provides a strategy to combine phototherapy with immunotherapy to enhance the therapeutic efficiency and further inhibit metastasis of tumors.


Assuntos
Antineoplásicos/administração & dosagem , Neoplasias da Mama/tratamento farmacológico , Cobre/química , Sistemas de Liberação de Medicamentos , Temperatura Alta , Nanopartículas Metálicas/administração & dosagem , Fotoquimioterapia/métodos , Animais , Antineoplásicos/química , Apoptose , Neoplasias da Mama/metabolismo , Neoplasias da Mama/patologia , Proliferação de Células , Citosina/química , Liberação Controlada de Fármacos , Feminino , Guanina/química , Humanos , Imunoterapia , Nanopartículas Metálicas/química , Camundongos , Camundongos Endogâmicos BALB C , Camundongos Nus , Óxidos/química , Fosfatos/química , Linfócitos T Citotóxicos/efeitos dos fármacos , Linfócitos T Citotóxicos/imunologia , Células Tumorais Cultivadas , Ensaios Antitumorais Modelo de Xenoenxerto
8.
Waste Manag ; 103: 361-369, 2020 Feb 15.
Artigo em Inglês | MEDLINE | ID: mdl-31923843

RESUMO

Traditional cementation technique is insufficient in making municipal solid waste incineration (MSWI) fly ashes meet the permissible leaching threshold. Aluminum supplementation and electrokinetic (EK) activation were combinedly incorporated into the traditional solidification pathway to enhance the geopolymerization and the immobilization of Pb and Cd in MSWI fly ashes in this study. The aluminum addition remarkably affected the geopolymer formation. The minimum toxicity leaching as well as the maximum compressive strength were achieved at the combination of the voltage gradient of 1.0 V/cm, the proposing time of 48 or 72 h, the mass ratio of alkali activator to fly ash of 11.5%, and the modulus of 2.1. Chloride reduction in the mortar obtained during the EK process increased the negative charge relativity of oligomers. The leaching concentrations of Pb and Cd from the geopolymer were successfully predicted by a linear model based on the compressive strengths at 28 d. Higher reaction degree was found in the EK-activated mortar in the geopolymerization kinetics. Aluminum supplementation had induced the production of some amorphous aluminosilicate minerals including Al6Si2O13, CaAl2Si2O8·4H2O, Ca2Al3(Si3O12)OH, Ca2Al(OH)7·3H2O, and Ca4Al2O6Cl2·10H2O during the EK process. Larger particles observed in the EK-treated specimen directly verified the EK-activated pozzolanic reactions.


Assuntos
Metais Pesados , Eliminação de Resíduos , Alumínio , Carbono , Cinza de Carvão , Suplementos Nutricionais , Incineração , Material Particulado , Resíduos Sólidos
9.
J Mater Chem B ; 8(5): 935-944, 2020 02 07.
Artigo em Inglês | MEDLINE | ID: mdl-31912837

RESUMO

Multifunctional nanotheranostic systems with both therapeutic and imaging functions are highly desired for the development of more effective and less toxic anti-tumor drugs. Herein, a simple but effective method is reported to fabricate a novel PCN-CuS-FA-ICG-based nanoplatform for dual-modal imaging-guided synergistic photothermal/photodynamic therapy. Porphyrinic metal-organic frameworks with CuS NPs are obtained in aqueous solution via a simple post-synthesis strategy. Furthermore, to obtain a more effective therapy, indocyanine green (ICG) was incorporated into the multifunctional theranostic platform to promote the photothermal therapeutic effect. The as-prepared PCN-CuS-FA-ICG not only exhibits an excellent 1O2 generation efficiency under 650 nm irradiation to achieve remarkable photodynamic cell killing, but also presents outstanding photothermal conversion under 808 nm irradiation to destroy tumor tissues by hyperthermia. In particular, the nanotherapeutic agent realized fluorescence and thermal imaging dual-modal imaging-guided cancer treatment. Meanwhile, in vivo experiments confirmed the evident accumulation of nanoparticles (NPs) at local tumors, and tumor growth was inhibited obviously via synergistic photothermal/photodynamic therapy with negligible side effects.


Assuntos
Antineoplásicos/farmacologia , Corantes/farmacologia , Hipertermia Induzida , Estruturas Metalorgânicas/farmacologia , Nanopartículas/química , Fotoquimioterapia , Antineoplásicos/síntese química , Antineoplásicos/química , Proliferação de Células/efeitos dos fármacos , Sobrevivência Celular/efeitos dos fármacos , Corantes/síntese química , Corantes/química , Cobre/química , Cobre/farmacologia , Ensaios de Seleção de Medicamentos Antitumorais , Humanos , Verde de Indocianina/química , Verde de Indocianina/farmacologia , Raios Infravermelhos , Teste de Materiais , Estruturas Metalorgânicas/síntese química , Estruturas Metalorgânicas/química , Imagem Óptica , Tamanho da Partícula , Oxigênio Singlete/análise , Oxigênio Singlete/metabolismo , Propriedades de Superfície , Células Tumorais Cultivadas
10.
Adv Mater ; 31(52): e1904997, 2019 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-31721331

RESUMO

Cancer immunotherapy has achieved promising clinical responses in recent years owing to the potential of controlling metastatic disease. However, there is a limited research to prove the superior therapeutic efficacy of immunotherapy on breast cancer compared with melanoma and non-small-cell lung cancer because of its limited expression of PD-L1, low infiltration of cytotoxic T lymphocytes (CTLs), and high level of myeloid-derived suppressor cells (MDSCs). Herein, a multifunctional nanoplatform (FA-CuS/DTX@PEI-PpIX-CpG nanocomposites, denoted as FA-CD@PP-CpG) for synergistic phototherapy (photodynamic therapy (PDT), photothermal therapy (PTT) included) and docetaxel (DTX)-enhanced immunotherapy is successfully developed. The nanocomposites exhibit excellent PDT efficacy and photothermal conversion capability under 650 and 808 nm irradiation, respectively. More significantly, FA-CD@PP-CpG with no obvious side effects can remarkably inhibit the tumor growth in vivo based on a 4T1-tumor-bearing mice modal. A low dosage of loaded DTX in FA-CD@PP-CpG can promote infiltration of CTLs to improve efficacy of anti-PD-L1 antibody (aPD-L1), suppress MDSCs, and effectively polarize MDSCs toward M1 phenotype to reduce tumor burden, further to enhance the antitumor efficacy. Taken together, FA-CD@PP-CpG nanocomposites offer an efficient synergistic therapeutic modality in docetaxel-enhanced immunotherapy for clinical application of breast cancer.


Assuntos
Docetaxel/química , Oligonucleotídeos/química , Linfócitos T Citotóxicos/imunologia , Animais , Anticorpos Monoclonais Humanizados/química , Anticorpos Monoclonais Humanizados/uso terapêutico , Linhagem Celular Tumoral , Sobrevivência Celular/efeitos dos fármacos , Cobre/química , Docetaxel/farmacologia , Docetaxel/uso terapêutico , Portadores de Fármacos/química , Ácido Fólico/química , Humanos , Imunoterapia , Lasers , Camundongos , Nanocompostos/química , Fototerapia , Polietilenoimina/química , Protoporfirinas/química , Espécies Reativas de Oxigênio/metabolismo , Linfócitos T Citotóxicos/citologia , Linfócitos T Citotóxicos/metabolismo , Neoplasias de Mama Triplo Negativas/metabolismo , Neoplasias de Mama Triplo Negativas/patologia , Neoplasias de Mama Triplo Negativas/terapia
11.
PLoS One ; 12(8): e0183395, 2017.
Artigo em Inglês | MEDLINE | ID: mdl-28829815

RESUMO

Several epidemiological studies have suggested that vitamin E could reduce the risk of uterine cervical neoplasm. However, controversial data were presented by different reports. Hence, we conducted a meta-analysis to assess the relationship between vitamin E and the risk of cervical neoplasia. We performed a comprehensive search of the PubMed, Embase and Cochrane databases through December 31, 2016. Based on a fixed-effects or random-effects model, the odds ratio (OR) and 95% confidence intervals (CIs) were calculated to assess the combined risk. Subgroup analyses and meta-regression were done to assess the source of heterogeneity. Subgroup analyses were performed according to survey ways, types of cervical neoplasia, study populations. A protocol was registered with PROSPERO (No. CRD42016036672). In total, 15 case-control studies were included, involving 3741 cases and 6328 controls. Our study suggested that higher category of vitamin E could reduce the cervical neoplasia risk (OR = 0.58, 95% CIs = 0.47-0.72, I2 = 83%). In subgroup-analysis, both vitamin E intake and blood levels of vitamin E had a significant inverse association with the risk of cervical neoplasm. Additionally, we found the same relationship between vitamin E and cervical neoplasia among different populations and types of cervical neoplasia. Meta-regression showed that none of the including covariates were significantly related to the outcomes. No evidence of publication bias was observed. In conclusion, vitamin E intake and blood vitamin E levels were inversely associated with the risk of cervical neoplasia.


Assuntos
Suplementos Nutricionais , Neoplasias do Colo do Útero/epidemiologia , Vitamina E/administração & dosagem , Estudos de Casos e Controles , Feminino , Humanos , Viés de Publicação , Neoplasias do Colo do Útero/prevenção & controle
12.
Biomed Res Int ; 2017: 8619385, 2017.
Artigo em Inglês | MEDLINE | ID: mdl-28210626

RESUMO

Expedited bone tissue engineering employs the biological stimuli to harness the intrinsic regenerative potential of skeletal muscle to trigger the reparative process in situ to improve or replace biological functions. When genetically modified with adenovirus mediated BMP2 gene transfer, muscle biopsies from animals have demonstrated success in regenerating bone within rat bony defects. However, it is uncertain whether the human adult skeletal muscle displays an osteogenic potential in vitro when a suitable biological trigger is applied. In present study, human skeletal muscle cultured in a standard osteogenic medium supplemented with dexamethasone demonstrated significant increase in alkaline phosphatase activity approximately 24-fold over control at 2-week time point. More interestingly, measurement of mRNA levels revealed the dramatic results for osteoblast transcripts of alkaline phosphatase, bone sialoproteins, transcription factor CBFA1, collagen type I, and osteocalcin. Calcified mineral deposits were demonstrated on superficial layers of muscle discs after an extended 8-week osteogenic induction. Taken together, these are the first data supporting human skeletal muscle tissue as a promising potential target for expedited bone regeneration, which of the technologies is a valuable method for tissue repair, being not only effective but also inexpensive and clinically expeditious.


Assuntos
Proteína Morfogenética Óssea 2/genética , Regeneração Óssea/genética , Músculo Esquelético/metabolismo , Osteogênese/genética , Engenharia Tecidual , Adenoviridae/genética , Adulto , Fosfatase Alcalina/biossíntese , Fosfatase Alcalina/genética , Animais , Proteína Morfogenética Óssea 2/biossíntese , Diferenciação Celular/genética , Colágeno Tipo I/biossíntese , Colágeno Tipo I/genética , Dexametasona/administração & dosagem , Feminino , Humanos , Sialoproteína de Ligação à Integrina/biossíntese , Sialoproteína de Ligação à Integrina/genética , Masculino , Células-Tronco Mesenquimais/metabolismo , Pessoa de Meia-Idade , Músculo Esquelético/citologia , Osteoblastos/efeitos dos fármacos , Osteocalcina/biossíntese , Osteocalcina/genética , Ratos
13.
Artigo em Inglês | MEDLINE | ID: mdl-27895695

RESUMO

Aims. To observe the effects of Guizhi Fuling Capsule (GZFLC) drug serum on uterine leiomyoma cells and explore its mechanism. Main Methods. Sixty Sprague Dawley rats were randomly divided into two groups (normal saline lavage group and GZFLC lavage group), then, respectively, blank serum and GZFLC drug serum were collected, and finally human uterine leiomyoma cells were treated. Human leiomyoma tissues were collected from 20 patients who underwent uterine leiomyomas operations, and leiomyoma cells were primary cultured. The leiomyoma cells were treated by GZFLC drug serum in different concentrations (10%, 20%, and 30%) and variable treatment time (12 h, 24 h, 36 h, 48 h, and 72 h). Cell proliferation was observed using CCK8 assay. Flow cytometry and Annexin V/PI were used to assay the effects of GZFLC drug serum on cell apoptosis. Western blot analysis was used to assay the effects of GZFLC drug serum on TSC2, FOXO, and 14-3-3γ expression in uterine leiomyoma cells. Key Findings. In the concentrations of 10%~30%, GZFLC drug serum could inhibit proliferation of leiomyoma cells in dose-dependent manner; at the time of 36 h, cell inhibition rate was at the peak; GZFLC drug serum could induce apoptosis of leiomyoma also in a dose-dependent manner, and apoptosis rate quickly achieved maximum at 12 h time points, and then second apoptosis peak appeared at 36 h. Compared to nontreatment group, TSC2, FOXO, and 14-3-3γ expressions in drug serum group were significantly changed after 12 h treatment. Significance. GZFLC drug serum can efficiently inhibit the proliferation and induce apoptosis of leiomyoma cells, which is related to the 14-3-3γ pathway.

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