Se transformation and removal by a cattail litter treatment system inoculated with sulfur-based denitrification sludge: Role of the microbial community composition under various temperature and aeration conditions.

With a narrow margin between deficiency and toxicity, rising levels of selenium (Se) are threatening aquatic ecosystems. To investigate the role of microorganisms in Se bioremediation, a cattail litter system inoculated with the sulfur-based denitrification sludge was conducted. The results show the litter, as a carrier and nutrient source for bacteria, efficiently removed Se by ~ 97.0% during a 12-d treatment with water circulating. As the major removal pathways, immobilization rates of selenite were ~ 2.9-fold higher than selenate, and the volatilization, contributing to ~ 87.7% of the total Se removal, was significantly correlated with temperature (positively) and oxidation-reduction potential (ORP; negatively). Using X-ray absorption spectroscopy to speciate litter-borne Se, more Se0 formed without aeration due to abundant Se-reducing bacteria, among which Azospira and Azospirillum were highly related to the removal of both Se oxyanions, while Desulfovibrio, Azoarcus, Sulfurospirillum, Thauera, Geobacter, Clostridium, and Pediococcus were the major contributors to selenate removal. Overall, our study suggests microbial Se metabolism in the litter system was significantly affected by temperature and ORP, which could be manipulated to enhance Se removal efficiency and the transformation of selenate/selenite into low toxic Se0 and volatile Se, reducing risks posed by the residual Se in the system.

Selenium uptake, volatilization, and transformation by the cyanobacterium Microcystis aeruginosa and post-treatment of Se-laden biomass.

With a narrow margin between beneficial and toxic effects, selenium (Se) is of great concern due to its increasing level in aquatic environments. The accumulation and transformation of Se by the cyanobacterium Microcystis aeruginosa and effects of nutrients, particularly sulfate, were investigated. The nutrient-deprived cyanobacterium removed water-borne selenate (82.2 ± 0.93%) faster than selenite (58.9 ± 1.77%), with 86.0 ± 1.41% and 77.2 ± 1.00%, respectively, of the Se accumulated in the biomass and the rest volatilized. When supplied with excess nutrients, the Se accumulation and volatilization rates were significantly inhibited, with the removal efficiency dropping to 50.2 ± 2.59% and 7.37 ± 0.93% for selenite and selenate, respectively. When M. aeruginosa was tested with inadequate, appropriate, and adequate levels of sulfate, Se uptake decreased with increasing sulfate concentrations, particularly for selenate (from 34.1 to 4.81%). Using X-ray absorption near-edge structure to speciate biomass Se, selenite and selenate were transformed to organo-Se (87.3-100%), with or without nutrients present, suggesting M. aeruginosa could efficiently reduce Se oxyanions to more bioavailable forms. With increasing sulfate levels (5.0 and 10.0 mg S/L), percentages of SeMet converted from selenite decreased by 28.2-33.0%, with 19.1-33.2% as elemental Se, while organo-Se remained dominant (93.6-95.1%) in selenate-treated M. aeruginosa. Transmission electron microscopy shows structural damage in the cell wall at exposure to selenite (1600 µg Se/L), with the intracellular structure intact. To prevent Se biomagnification along aquatic food chains, the Se-laden biomass was combusted as a post-treatment, leading to a significant reduction in Se content (∼99.2%) and Se bioavailability, with inorganic Se (45.0-70.5%) predominant in the residue.

Remediation of selenium-contaminated soil through combined use of earthworm Eisenia fetida and organic materials.

Selenium (Se)-polluted soils pose serious threats to terrestrial ecosystems through food chains. This study evaluated the use of earthworm Eisenia fetida and organic materials for Se remediation. The greater mortality (6.7%) and weight loss (28.6%) were observed for earthworms exposed to selenate than selenite at 20 mg Se Kg-1 over 21 d, while selenate was taken up 2.5-fold faster than selenite. Compared with peat moss, Se accumulation increased by 119% in selenite-exposed earthworms supplied with cow manure. Earthworm activity caused greater reduction in selenite (17.4%), with little impact on selenate-treated soil. X-ray absorption spectroscopy speciation analysis shows 87-91% of tissue Se was transformed to organo-Se, i.e., SeMet and SeCys, in earthworms exposed to either selenite or selenate, posing great risks to their predators. The study also found selenium increased bacterial diversity in earthworm casts, while greater relative abundances (~37.57%) of functional genera were obtained for selenite. Over 24 h, two bacteria strains, Bacillus cereus and Aeromonas encheleia, isolated from casts, rapidly reduced selenite by ~94%, compared to ~25% for selenate. Elemental Se was present only in strains (~27%), casts (~11%) and worm-inhabited soil (~2.7%) of selenite treatments, suggesting earthworm gut microbiota could buffer earthworms and other soil fauna from selenite toxicity.

Selenium removal by clam shells and gravels amended with cattail and reed litter.

Increasing selenium (Se) levels in aquatic environments raise concerns all over the world. This study investigated effects of organic amendments (cattail and reed litter) and porous media (gravels and clam shells) on Se removal efficiency of horizontal subsurface flow constructed wetlands. Our results show clam shells reduced Se (by mass) up to 2.4-fold faster than gravels within 19 days. Using clam shells as the sole substrate, 96.3% removal efficiency was obtained for cattail litter as an amendment, compared to 88.7% for reed litter over 10 days, although the latter released carbon and nitrogen at least 1.4-fold faster than the former. Meanwhile, speciation analysis suggests Se0 (~75%) and organo-Se (~94%) dominated the biofilms on shells and plant litter, respectively, as substrates. Overall, this study suggests clam shells and cattail litter as an effective medium and carbon source, respectively, can enhance microbial Se removal without posing risks to wildlife health.

Mutual effects of selenium and chromium on their removal by Chlorella vulgaris and associated toxicity.

The release of selenium (Se) and chromium (Cr) into the environment from anthropogenic activities has posed a hazard to aquatic ecosystems. In this study, we used Chlorella vulgaris for Se/Cr bioremediation and evaluated their mutual effects on the removal efficiency. Our results found C. vulgaris highly effective in removing selenite-Se(IV) (49.5 ± 1.9%), selenate-Se(VI) (93.0 ± 0.5%), chromic nitrate-Cr(III) (89.0 ± 3.2%) and dichromate-Cr(VI) (88.1 ± 1.3%) over a 72 h period. Cr(VI) significantly impeded Se removal, particularly for selenate, due to competition between both for algal uptake, whereas Cr(III) obviously enhanced Se removal, increasing Se volatilization by ~29%. Similarly, Se significantly increase Cr removal rates, with a maximum of 94.6 ± 0.2% for the algal co-exposed to Se(IV) and Cr(III). To reduce residual pollutants in the alga, we applied combustion as a post-treatment to burn off >99% of the biomass Se for all Se treatments, whereas most of the biomass Cr (54.7-81.6%) remained in the ash at significantly higher levels (~7430 µg Cr/g DW). For toxicity, our speciation analysis found organo-Se (SeCys and SeMet) dominant in the alga exposed to Se, particularly selenite. No Cr(VI) but Cr(III) forms were detected in all Cr-exposed alga. Elemental Se disappeared from all Se-exposed alga in the presence of Cr(VI), while Se resulted in the emergence of Cr-acetate in all Cr(III)-treated alga. After combustion, mineral Se, particularly elemental Se dominated most of the ash; likewise, elemental Cr, along with Cr2O3, was found in all the ash. Overall, our research would contribute to developing a low ecotoxic algal treatment system for Se/Cr contaminated water.

Trophic transfer and biotransformation of selenium in the mosquito (Aedes albopictus) and interactive effects with hexavalent chromium.

As an essential micronutrient for animals with a narrow range between essentiality and toxicity, selenium (Se) usually coexists with chromium (Cr) in contaminated aquatic environments. This study investigated effects of three diets (Microcystis aeruginosa, Chlorella vulgaris and biofilms) exposed to Se or/and Cr on Aedes albopictus as a vector for the aquatic-terrestrial transfer of Se and Cr. Se(IV)-exposed mosquitoes concentrated Se up to 66-fold faster than Se(VI)-exposed ones, corresponding to the greater Se enrichment in Se(IV)-treated diets. Analysis using synchrotron-based X-ray absorption spectroscopy (XAS) showed that Se(0) (61.9-74.6%) dominated Se(VI)-exposed mosquitoes except for the C. vulgaris-fed larvae (organo-Se, 94.0%), while organo-Se accounted for 93.3-100.0% in Se(IV)-exposed mosquitoes. Cr accumulation in larvae (56.40-87.24 µg Cr/g DW) or adults (19.41-50.77 µg Cr/g DW) was not significantly different among all Cr(VI) treatments, despite varying diet Cr levels. With Cr(0) being dominant (57.7-94.0%), Cr(VI)-exposed mosquitoes posed little threat to predators. Although mosquitoes exposed to Se or Cr had shorter wings, adults supplied with C. vulgaris or biofilms co-exposed to Se(VI) and Cr(VI) had wings significantly (1.1-1.2 fold) longer than Se(VI) only exposed ones. Overall, our study reveals the role of Ae. albopictus in transferring waterborne Se and Cr from the contaminated aquatic ecosystem to the terrestrial ecosystem with the resulting eco-risks to wildlife in both ecosystems.

Enhancement of a constructed wetland water treatment system for selenium removal.

Selenium (Se) is essential to most animals, whereas the gap between necessity and toxicity is narrow. Our previous work showed constructed wetlands were a promising solution to Se contamination in aquatic habitats. This study further examined effects of organic amendments and hydrologic regimes on Se removal by constructed wetlands. Our results suggest the removal efficiency exceeded 94% within 8 days for the systems with moderate and low organic carbon contents in the substrate, as a 98% removal of Se was obtained in three weeks for the system subjected to the 2-day wet/dry cycle. To mimic field wetlands, a litter layer was added to the cattail treatment system, which reduced waterborne Se much more rapidly than control, achieving a 77% removal of Se within 4 days. XAS results show all sediment Se was transformed to Se0 in the presence of litter, as SeMet (47%) dominated the Se adsorbed by the litter. The findings indicate the Se removal capacity of a constructed wetland would improve over time, especially via Se volatilization into the atmosphere and Se stabilization in the sediment with litter accumulating at the surface. Another mesocosm experiment showed the cattail floating system effectively removed Se, particularly selenate, by 99% in 48 h. To confirm that high performance, seven constructed wetland types were set up for comparison. The results show the cattail floating system was most effective in Se removal (93-100% at around 35 °C in summer and 51-100% at about 5 °C in winter). More research is needed to test the floating system under more field conditions and investigate the biomagnification and biotransformation of the removed Se along food chains. Seven constructed wetland types were set up for comparison.

Interactive effects of earthworm Eisenia fetida and bean plant Phaseolus vulgaris L on the fate of soil selenium.

Selenium (Se) is an essential micronutrient for animals with a narrow margin between essentiality and toxicity. Se toxicity is largely related to inorganic forms of Se in soil, i.e., selenite and selenate that enter food chains through plant uptake, threatening higher trophic level organisms. This experiment investigated effects of earthworm activity on Se bioavailability in soil and the subsequent plant uptake, using earthworm Eisenia fetida and bean plant Phaseolus vulgaris L, both exposed to either selenite or selenate at 1 or 4 mg Se kg-1 for 16 weeks. Plants took up selenate (up to 221-fold) faster than selenite, with up to 84% of the Se rapidly transported to shoots. In the presence of earthworms, Se accumulation obviously increased for selenate-supplied plants, leading to an up to 4% increase in Se translocation factor for all treatments except for 1 mg kg-1 selenite treatment. Earthworms also concentrated Se faster in tissues (up to 274 mg kg-1 DW) at exposure to selenate. For Se toxicity, Se speciation analysis was conducted on the plants and earthworms using XAS. Compared to worm-free treatments, the percentage of organo-Se, i.e., SeMet and CysSeSeCys, increased in beans (up to 34%) in the presence of earthworms for selenate, while the elemental Se portion was significantly reduced or absent, opposite to the results for selenite. Surprisingly, elemental Se (up to 65%) dominated earthworms, regardless of the form of Se supplied. In conclusion, earthworms clearly enhanced Se uptake and translocation in plants, leading to elevated Se levels in shoots. To prevent resulting hazards to humans and other animals, caution should be taken while consuming the shoots, particularly beans, harvested from the Se contaminated soil where earthworm activity is high. Finally, the significant reduction in soil Se suggests phytoextraction of Se from the soil could be improved using earthworms as an aid to plants.

Selenium removal and biotransformation in a floating-leaved macrophyte system.

Selenium (Se) is an essential micronutrient for animals with a relatively narrow margin between essentiality and toxicity. To evaluate Se removal efficiency by a constructed wetland treatment system and its potential eco-risk, a floating-leaved macrophyte system was constructed, consisting of three main trophic levels. Over 21-d treatment, water Se concentration was gradually reduced by 40.40%, while 24.03% and 74.41% of the removed Se were found in the plant Nymphoides sp. and sediment, respectively. Among plant tissues, roots accumulated the highest Se level, although the greatest total Se was found in stems, followed by leaves, roots and rhizomes. X-ray absorption spectroscopy revealed that 82.65% of the absorbed selenite by the plants was biotransformed to other forms, as organo-Se species accounted for 45.38% of the Se retained in the sediment, which was primarily responsible for the entry of Se into the detritus food chain. The proportion of organo-Se compounds increased with trophic levels from sediments to fish, indicating, instead of direct uptake of selenite, the food chain transfer and biotransformation of Se may serve as a key exposure route for Se in aquatic organisms. When exposed to organo-Se compounds, i.e., SeCys and SeMet, the plants, shrimp and fish tended to accumulate more Se. However, the greater trophic transfer factor was obtained for selenate, leading to higher Se levels accumulated in fish. Overall, in addition to key mechanisms involved in Se removal, our research also provides a much better understanding of the potential eco-risk that may be posed by the floating-leaved plant system for bioremediation of Se via food chain transfer and biotransformation, paving the way for a low eco-toxic treatment system for Se remediation.

Development of an algal treatment system for selenium removal: Effects of environmental factors and post-treatment processing of Se-laden algae.

In developing an algal treatment system, selenium (Se) removal efficiency by Chlorella vulgaris was evaluated under various conditions such as Se concentration, algal density, temperature and pH. A maximum removal efficiency plateau of ∼90% was observed between 1000-3000 µg Se/L while the tolerance of Se toxicity was found at 6000 µg Se/L. C. vulgaris of 0.75 g DW/L showed the highest removal efficiency (84%), and volatilization was dominant below 1.37 g DW/L. Se volatilization was two times higher at 25 °C than at 20 °C in the first 24 h. Moreover, the highest removal efficiency (77%) was obtained at pH 8.0, compared to 66.5% at pH 6.5 and 40% at pH 10.0. To prevent ecotoxicity, Se laden algae were further burned to ashes or filtered out by Anodonta woodiana. After burning, biomass Se was reduced by 99%, with organo-Se entirely converted into inorganic Se, lowering Se bioavailability. A. woodiana removed 54% of Se in 24 h, leading to Se bioaccumulation in soft tissues, which may serve as dietary Se supplements for human health. Our results suggest the cleanup of Se-contaminated water from either agricultural runoff or industrial discharge could be achieved using an algal treatment system with minimum potential ecotoxicity.

Relationship between selenium removal efficiency and production of lipid and hydrogen by Chlorella vulgaris.

In our previous studies, Chlorella vulgaris had proven highly efficient in removing selenium (Se) from water, while the disposal of Se containing in algal biomass was still an issue of concern. Firstly, this research suggests algal Se could be released back to water, posing risks to aquatic wildlife. Thus, we further explored the possibility of using C. vulgaris to remove Se and produce lipid and hydrogen simultaneously. Our results show the higher percentage of saturated fatty acids, especially palmitic acid, was found in the sulfur (S) deprived algae exposed to either selenate or selenite, although the highest lipid content (21.9%) was found in the selenite treated algae in full BG11 medium. In addition, compared with the Se free algae, hydrogen production rate was 2.1- and 4.3-fold higher for the selenate and selenite treated algae, respectively. Se removal efficiency achieved by the selenite treated algae through accumulation and volatilization was 2.3-fold higher than the selenate treatment under hypoxic condition with S deprived, which is in contrast to the results obtained under aerobic conditions.

Intermittent operating characteristics of an ecological soil system with two-stage water distribution for wastewater treatment.

Ecological soil systems (ESSs) are usually used to remove nitrogen from wastewater. Due to the poor denitrification performance of traditional ecological soil systems (ESSs), this study proposes a two-stage water distribution system to improve the nitrogen removal. The effects of different distribution ratios on the system treatment effect were studied in an intermittent operation mode. After determining the optimal distribution ratio and intermittent operation conditions, the dynamics of system inflow, outflow, and nitrogen removal were monitored. Theoretical analysis of the denitrification mechanism was carried out. The results showed that the optimum water distribution ratio was 2: 1, and a mean total nitrogen removal rate of 60.42% was achieved, which is 23.09% greater than that is typically achieved by the single-section ecological system. Under optimum distribution ratio conditions, the system also demonstrated effective removal of chemical oxygen demand (COD), total phosphorus (TP) and ammonia nitrogen (NH4+-N), allowing the effluent to satisfy China's urban sewage treatment plant level B emission standards.

Removal of selenium containing algae by the bivalve Sinanodonta woodiana and the potential risk to human health.

Selenium (Se) is an essential micronutrient for animals and humans with a relatively narrow margin between nutritional essentiality and potential toxicity. Even though our previous studies have demonstrated algae could efficiently remove Se, mainly through volatilization, concern is raised about eco-risks posed by the remaining Se in algae. Here, Sinanodonta woodiana was investigated as a biofilter for the removal of Se-containing Chlorella vulgaris and for its potential risk to human health. Our results suggest filtration rates of S. woodiana were independent of Se levels in algal biomass, with a removal efficiency of between 60 and 78%. However, Se concentrations accumulated in mussels were significantly correlated with algal-borne Se levels, with a dietary assimilation efficiency ranging from 12% to 46%. Thus, a pilot biofiltration system was set up to assess uptake and depuration processes. The system was found to efficiently remove Se laden algae through the uptake by mussels, while 21% of Se in mussels could be depurated in 6 days. Among tissues, gills accumulated the highest Se concentration after assimilating algal-borne Se but shed Se compounds in the fastest pace during depuration. Health risks posed by consumption of mussels exposed to different sources of Se were further assessed. S. woodiana accumulated the highest Se concentration after exposure to waterborne SeMet, followed by dietary Se, selenite and control. The relatively higher Se levels were found in gills for all the treatments. After boiling, the most common method of cooking mussels, the greatest reduction in Se concentration occurred in mantle for the control and dietary Se groups and in muscle for the SeMet and selenite treatments. Therefore, within the safe limits, Se-containing mussels can be consumed as a dietary supplement. Overall, our research suggests incorporation of mussels into an algal treatment system can improve Se removal efficiency and also provide financial incentives for practitioners.

[Effects of Phosphorus on the Activity and Bacterial Community in Mixotrophic Denitrification Sludge].

Biological denitrification is now one of the most widely applied techniques to remove nitrogen from the aquatic environment, and mixotrophic denitrification has gained attention as it takes the characteristics of both heterotrophic and autotrophic denitrification. This study investigated the biological denitrification efficiency and the bacterial community structure of sludge sampled from a mixotrophic denitrification reactor, before and after adding a certain amount of phosphate. The results showed that the bacteria have the capability of denitrification even without phosphorus, but the addition of phosphorus could significantly improve the biomass and the denitrification activity. After phosphate was added, the autotrophic and heterotrophic denitrification activity increased to 0.056 mg·(L·min·g)-1 and 0.232 mg·(L·min·g)-1 on N/VSS, which was 2.9 and 3.9 times that of the sludge activity before phosphorus addition, respectively. The bacterial community structure illustrated that the denitrifiers increased remarkably from 13.47% to 44.82% and that the dominate bacteria have also changed. Meanwhile, the growth of autotrophic, heterotrophic, and mixtrophic bacteria were all improved significantly after phosphorus was added.

The influence of phosphorus on the autotrophic and mixotrophic denitrification.

Autotrophic and mixotrophic denitrification, two approaches of biological denitrification, have drawn more and more attention among the techniques to remove nitrogen from the aquatic environment. This study investigated the influence of phosphorus on the denitrification performance and bacterial community structure in the autotrophic and mixotrophic denitrification reactors. The activity test was applied to evaluate the variation of denitrification activity of autotrophic and mixotrophic sludge before and after phosphorus addition. High-throughput sequencing was used to analyze the change of bacterial community structure. The results showed that NO3--N removal efficiency of autotrophic and mixotrophic denitrification process increased by 40 and 35%, respectively, after phosphorus addition. The sludge denitrification activity of autotrophic and mixotrophic sludge was enhanced significantly. And phosphorus addition could greatly improve the proportion of denitrifying bacteria in both autotrophic (from 11.83 to 64.31%) and mixotrophic denitrifying sludge (from 13.59 to 45.12%). Overall, phosphorus addition could greatly improve the autotrophic and mixotrophic denitrification ability in the phosphorus deficient surface water.

Integrated ecological floating bed treating wastewater treatment plant effluents: effects of influent nitrogen forms and sediments.

In recent years, the treatment of wastewater treatment plant (WWTP) effluent has gained increasing attention. However, researches on the relationships between nitrogen forms and nitrogen removal efficiency are very limited. Based on the fact that the nitrogen forms in the WWTP effluent may vary as the season changes, the nitrogen removal efficiencies of an integrated ecological floating bed (IEFB) was studied under different influent nitrogen forms. In addition, the effects of sediments in the system were also quantified during the experiment. Results showed that the total nitrogen (TN) removal rates of the IEFB were 25.61 ± 5.72% and 60.03 ± 7.00%, respectively, when the main influent nitrogen forms are nitrate and ammonia. The sediments in the system also played vital roles in the removal processes: when the sediments were covered with a polyethylene membrane, the total nitrogen (TN) removal rate of the system dropped from 27.86 ± 5.53% to 14.78 ± 4.97%, and the total phosphorus (TP), from 58.77 ± 6.20% to 33.51 ± 25.52%.

Comparison of heterotrophic and autotrophic denitrification processes for nitrate removal from phosphorus-limited surface water.

Phosphorus (P) limitation has been demonstrated for micro-polluted surface water denitrification treatment in previous study. In this paper, a lab-scale comparative study of autotrophic denitrification (ADN) and heterotrophic denitrification (HDN) in phosphorus-limited surface water was investigated, aiming to find out the optimal nitrogen/phosphorus (N/P) ratio and the mechanism of the effect of P limitation on ADN and HDN. Furthermore, the optimal denitrification process was applied to the West Lake denitrification project, aiming to improve the water quality of the West Lake from worse than grade V to grade IV (GB3838-2006). The lab-scale study showed that the lack of P indeed inhibited HDN more greatly than ADN. The optimal N/P ratio for ADN and HDN was 25 and a 0.15 mg PO43--P L-1 of microbial available phosphorus (MAP) was observed. P additions could greatly enhance the resistance of ADN and HDN to hydraulic loading shock. Besides, The P addition could effectively stimulate the HDN performance via enriching the heterotrophic denitrifiers and the denitrifying phosphate-accumulating organisms (DNPAOs). Additionally, HDN was more effective and cost-effective than ADN for treating P-limited surface water. The study of the full-scale HDBF (heterotrophic denitrification biofilter) indicated that the denitrification performance was periodically impacted by P limitation, particularly at low water temperatures.

Decomposition characteristics of three different kinds of aquatic macrophytes and their potential application as carbon resource in constructed wetland.

Decomposition of aquatic macrophytes usually generates significant influence on aquatic environment. Study on the aquatic macrophytes decomposition may help reusing the aquatic macrophytes litters, as well as controlling the water pollution caused by the decomposition process. This study verified that the decomposition processes of three different kinds of aquatic macrophytes (water hyacinth, hydrilla and cattail) could exert significant influences on water quality of the receiving water, including the change extent of pH, dissolved oxygen (DO), the contents of carbon, nitrogen and phosphorus, etc. The influence of decomposition on water quality and the concentrations of the released chemical materials both followed the order of water hyacinth > hydrilla > cattail. Greater influence was obtained with higher dosage of plant litter addition. The influence also varied with sediment addition. Moreover, nitrogen released from the decomposition of water hyacinth and hydrilla were mainly NH3-N and organic nitrogen while those from cattail litter included organic nitrogen and NO3--N. After the decomposition, the average carbon to nitrogen ratio (C/N) in the receiving water was about 2.6 (water hyacinth), 5.3 (hydrilla) and 20.3 (cattail). Therefore, cattail litter might be a potential plant carbon source for denitrification in ecological system of a constructed wetland.

Development of an algal treatment system for Se removal: Effects of light regimes, nutrients, sulfate and hypersalinity.

Selenium (Se) exposure poses potential risks to wildlife at the Salton Sea. Our previous research suggests Chlorella sp. be highly efficient at absorbing and volatilizing Se. In developing an algal treatment system for Se removal, this study further evaluated the performance under the conditions to be encountered in the field using Chlorella pyrenoidosa and Chlorella vulgaris. The results show the algal Se removal efficiency was little affected by photoperiod, yet volatilization became relatively greater in dark/light cycles over a longer term. The rates of Se absorption and volatilization by C. vulgaris were 88% and 77% more, respectively, in the DI water, while C. pyrenoidosa acted oppositely, indicating C. vulgaris will perform better in Se removal if nutrient levels are reduced in advance. The presence of sulfate reduced biomass Se, especially through volatilization, by 8% for C. vulgaris, lessening potential ecotoxicity. Finally, C. vulgaris released biomass Se back to the water column under hypersaline conditions, leading to a 6% increase in water Se concentrations. These results suggest C. vulgaris be the best alga for the treatment of Se laden river water in the Salton Sea area, yet a filtering system is required to prevent Se containing algae from entering food chains.

Application of novel catalytic-ceramic-filler in a coupled system for long-chain dicarboxylic acids manufacturing wastewater treatment.

To gain systematic technology for long-chain dicarboxylic acids (LDCA) manufacturing wastewater treatment, catalytic micro-electrolysis (CME) coupling with adsorption-biodegradation sludge (AB) process was studied. Firstly, novel catalytic-ceramic-filler was prepared from scrap iron, clay and copper sulfate solution and packed in the CME reactor. To remove residual n-alkane and LDCA, the CME reactor was utilized for LDCA wastewater pretreatment. The results revealed that about 94% of n-alkane, 98% of LDCA and 84% of chemical oxygen demand (COD) were removed by the aerated CME reactor at the optimum hydraulic retention time (HRT) of 3.0 h. In this process, catalysis from Cu and montmorillonites played an important role in improving the contaminants removal. Secondly, to remove residual COD in the wastewater, AB process was designed for the secondary biological treatment, about 90% of the influent COD could be removed by biosorption, bio-flocculation and biodegradation effects. Finally, the effluent COD (about 150 mg L(-1)) discharged from the coupled CME-AB system met the requirement of the national discharged standard (COD ≤ 300 mg L(-1)). All of these results suggest that the coupled CME-AB system is a promising technology due to its high-efficient performance, and has the potential to be applied for the real LDCA wastewater treatment.